About: Prolonged gene expression in mouse lung endothelial cells following transfection with Epstein

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About: Prolonged gene expression in mouse lung endothelial cells following transfection with Epstein–Barr virus-based episomal plasmid Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Prolonged gene expression in mouse lung endothelial cells following transfection with Epstein–Barr virus-based episomal plasmid
Creator	Zhang, J Huang, L Li, S Ma, Z Alber, S Mazda, O Pitt, B Satoh, E Tang, Z-L Watkins, S Wilson, A
topic	covid:2c044a2a0e1c5d33c536a1e2b42875da9cfeb13c#this
Source	PMC
abstract	The development of a strategy to deliver a gene to pulmonary endothelium will be useful for gene function study and for pulmonary gene therapy. Cationic lipidic vectors are efficient in gene transfer to pulmonary endothelium via the vascular route; however, gene expression is transient and lasts for only a few days. In this study, we show that pulmonary gene transfer via cationic lipidic vectors can be significantly improved using an Epstein–Barr virus (EBV)-based expression plasmid. Systemic administration of cationic liposomes followed by the EBV-based plasmid led to gene expression in the lung that lasted for more than 3 weeks. Prolonged and high levels of gene expression can also be obtained in primary mouse lung endothelial cells (MLEC) following lipofection with an EBV-based plasmid. These results suggest the utility of this gene transfer protocol in studying the expression of cloned genes in lung endothelial cells and in pulmonary gene therapy.
has issue date	2003-04-17(xsd:dateTime)
bibo:doi	10.1038/sj.gt.3301958
bibo:pmid	12704423
has license	no-cc
sha1sum (hex)	2c044a2a0e1c5d33c536a1e2b42875da9cfeb13c
schema:url	https://doi.org/10.1038/sj.gt.3301958
resource representing a document's title	Prolonged gene expression in mouse lung endothelial cells following transfection with Epstein–Barr virus-based episomal plasmid
has PubMed Central identifier	PMC7091729
has PubMed identifier	12704423
schema:publication	Gene Ther
resource representing a document's body	covid:2c044a2a0e1c5d33c536a1e2b42875da9cfeb13c#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/2c044a2a0e1c5d33c536a1e2b42875da9cfeb13c
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