About: Expression Cloning and Production of Human Heavy-Chain-Only Antibodies from Murine Transgenic Plasma Cells

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About: Expression Cloning and Production of Human Heavy-Chain-Only Antibodies from Murine Transgenic Plasma Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression Cloning and Production of Human Heavy-Chain-Only Antibodies from Murine Transgenic Plasma Cells
Creator	Drabek, Dubravka Grosveld, Frank Janssens, Rick Kloess, Johannes Rademaker, Rik Skehel, John Hayhurst, Andrew Henry, Kevin De Boer, Ernie Nilvebrant, Johan
Source	PMC
abstract	Several technologies have been developed to isolate human antibodies against different target antigens as a source of potential therapeutics, including hybridoma technology, phage and yeast display systems. For conventional antibodies, this involves either random pairing of VH and variable light (VL) domains in combinatorial display libraries or isolation of cognate pairs of VH and VL domains from human B cells or from transgenic mice carrying human immunoglobulin loci followed by single-cell sorting, single-cell RT-PCR, and bulk cloning of isolated natural VH–VL pairs. Heavy-chain-only antibodies (HCAbs) that naturally occur in camelids require only heavy immunoglobulin chain cloning. Here, we present an automatable novel, high-throughput technology for rapid direct cloning and production of fully human HCAbs from sorted population of transgenic mouse plasma cells carrying a human HCAb locus. Utility of the technique is demonstrated by isolation of diverse sets of sequence unique, soluble, high-affinity influenza A strain X-31 hemagglutinin-specific HCAbs.
has issue date	2016-12-19(xsd:dateTime)
bibo:doi	10.3389/fimmu.2016.00619
bibo:pmid	28066429
has license	cc-by
sha1sum (hex)	2c332ca9f08d552ef49e6945655e9059e82ba13d
schema:url	https://doi.org/10.3389/fimmu.2016.00619
resource representing a document's title	Expression Cloning and Production of Human Heavy-Chain-Only Antibodies from Murine Transgenic Plasma Cells
has PubMed Central identifier	PMC5165034
has PubMed identifier	28066429
schema:publication	Front Immunol
resource representing a document's body	covid:2c332ca9f08d552ef49e6945655e9059e82ba13d#body_text
is schema:about of	named entity 'ANTIBODIES' named entity 'human' named entity 'phage' named entity 'soluble' named entity 'combinatorial' named entity 'antibodies' named entity 'isolate' named entity 'production' named entity 'HUMAN' named entity 'HEAVY-CHAIN' named entity 'ISOLATED' named entity 'SORTED' named entity 'TECHNIQUE' named entity 'POTENTIAL' named entity 'LIBRARIES' named entity 'INFLUENZA A' named entity 'HUMAN IMMUNOGLOBULIN' named entity 'DISPLAY' named entity 'HCAB' named entity 'PAIRING' named entity 'OCCUR' named entity 'DEMONSTRATED' named entity 'LOCUS' named entity 'DIFFERENT' named entity 'UNIQUE' named entity 'LIGHT' named entity 'CELL' named entity 'TECHNOLOGY' named entity 'CARRYING' named entity 'HEAVY-CHAIN' named entity 'TRANSGENIC MOUSE' named entity 'BULK' named entity 'SOLUBLE' named entity 'SOURCE' named entity 'YEAST DISPLAY' named entity 'CELL SORTING' named entity 'CONVENTIONAL' named entity 'RT-PCR' named entity 'SPECIFIC' named entity 'THERAPEUTICS' named entity 'DIRECT' named entity 'TARGET' named entity 'PHAGE' named entity 'PRESENT' named entity 'PRODUCTION' named entity 'PAIRS' named entity 'SINGLE' named entity 'HEAVY' named entity 'SYSTEMS' named entity 'INCLUDING' named entity 'TECHNOLOGIES' named entity 'ISOLATE' named entity 'PLASMA CELLS' named entity 'IMMUNOGLOBULIN CHAIN' named entity 'PRODUCTION' named entity 'TRANSGENIC' named entity 'TRANSGENIC MICE' named entity 'EXPRESSION CLONING' named entity 'HIGH' named entity 'NATURAL' named entity 'VARIABLE' named entity 'FULLY' named entity 'LOCI' named entity 'HAVE' named entity 'STRAIN' named entity 'HERE' named entity 'RANDOM' named entity 'HUMAN' named entity 'AFFINITY' named entity 'DIVERSE' named entity 'POPULATION' named entity 'ANTIBODIES'

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