About: Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells
Creator	Li, Lei Yang, Xia Wu, Xinwei Gao, Guoquan Hong, Hua Jiang, Liyun Li, Qiaoyan Li, Xiangzhong Wu, Yejian Yue, Jinya
Source	Medline; PMC
abstract	BACKGROUND: Dengue viruses (DENs) are the wildest transmitted mosquito-borne pathogens throughout tropical and sub-tropical regions worldwide. Infection with DENs can cause severe flu-like illness and potentially fatal hemorrhagic fever. Although RNA interference triggered by long-length dsRNA was considered a potent antiviral pathway in the mosquito, only limited studies of the value of small interfering RNA (siRNA) have been conducted. RESULTS: A 21 nt siRNA targeting the membrane glycoprotein precursor gene of DEN-1 was synthesized and transfected into mosquito C6/36 cells followed by challenge with DEN. The stability of the siRNA in cells was monitored by flow cytometry. The antiviral effect of siRNA was evaluated by measurement of cell survival rate using the MTT method and viral RNA was quantitated with real-time RT-PCR. The presence of cells containing siRNA at 0.25, 1, 3, 5, 7 days after transfection were 66.0%, 52.1%, 32.0%, 13.5% and 8.9%, respectively. After 7 days incubation with DEN, there was reduced cytopathic effect, increased cell survival rate (76.9 ± 4.5% vs 23.6 ± 14.6%) and reduced viral RNA copies (Ct value 19.91 ± 0.63 vs 14.56 ± 0.39) detected in transfected C6/36 cells. CONCLUSIONS: Our data showed that synthetic siRNA against the DEN-1 membrane glycoprotein precursor gene effectively inhibited DEN-1 viral RNA replication and increased C6/36 cell survival rate. siRNA may offer a potential new strategy for prevention and treatment of DEN infection.
has issue date	2010-10-14(xsd:dateTime)
bibo:doi	10.1186/1743-422x-7-270
bibo:pmid	20946645
has license	cc-by
sha1sum (hex)	2c6d1fc20495ba6b8b43ffe06cc0d9bb80244d96
schema:url	https://doi.org/10.1186/1743-422x-7-270
resource representing a document's title	Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells
has PubMed Central identifier	PMC2965154
has PubMed identifier	20946645
schema:publication	Virol J
resource representing a document's body	covid:2c6d1fc20495ba6b8b43ffe06cc0d9bb80244d96#body_text
is schema:about of	named entity 'antiviral' named entity 'dengue virus' named entity 'CELLS' named entity 'ANTIVIRAL' named entity 'TRIGGERED BY' named entity 'VALUE' named entity 'FLU-LIKE ILLNESS' named entity 'DSRNA' named entity 'TROPICAL REGIONS' named entity 'LENGTH' named entity 'MOSQUITO' named entity 'SMALL INTERFERING RNA' named entity 'CAUSE' named entity 'BACKGROUND' named entity 'FATAL' named entity 'BORNE' named entity 'DENGUE VIRUS' named entity 'MOSQUITO' named entity 'LONG' named entity 'PATHOGENS' named entity 'STUDIES' named entity 'HEMORRHAGIC FEVER' named entity 'SEVERE' covid:arg/2c6d1fc20495ba6b8b43ffe06cc0d9bb80244d96 named entity 'SMALL INTERFERING RNA' named entity 'VIRUS REPLICATION' named entity 'PATHWAY' named entity 'CONSIDERED' named entity 'LIMITED' named entity 'WORLDWIDE' named entity 'DENS' named entity 'HAVE' named entity 'SUB-' named entity 'INHIBITORY EFFECT' named entity 'INFECTION' named entity 'RNA INTERFERENCE' named entity 'DENGUE VIRUSES' named entity 'TRANSMITTED' named entity 'TROPICAL' named entity 'viruses' named entity 'small interfering RNA' named entity 'pathogens' named entity 'sub-tropical' named entity 'mosquito' named entity 'sub-tropical' named entity 'viruses' named entity 'flu-like illness' named entity 'mosquito' named entity 'viral RNA' named entity 'DNA replication' named entity 'dsRNA' named entity 'genus' named entity 'viruses' named entity 'dengue virus' named entity 'gene' named entity 'exogenous' named entity 'Flavivirus' named entity 'studying gene function' named entity 'Qiagen' named entity 'dsRNA' named entity 'siRNA' named entity 'Guangzhou' named entity 'virus' named entity 'siRNA' named entity 'transfection' named entity 'siRNA' named entity 'Qiagen' named entity 'cytopathic effect' named entity 'RT-PCR' named entity 'positive control' named entity 'streptomycin' named entity 'exogenous'

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