About: Quantification of SARS-CoV-2 and cross-assembly phage (crAssphage) from wastewater to monitor coronavirus transmission within communities

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About: Quantification of SARS-CoV-2 and cross-assembly phage (crAssphage) from wastewater to monitor coronavirus transmission within communities Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Quantification of SARS-CoV-2 and cross-assembly phage (crAssphage) from wastewater to monitor coronavirus transmission within communities
Creator	Collins, Mary Fenty, Ariana Gentile, Karen Green, Hyatt Kmush, Brittany Larsen, David Middleton, Frank Wilder, Maxwell Zeng, Teng
topic	covid:2daf5bce6c08e45e6fccfeb43cac842ab44564c9#this
Source	MedRxiv
abstract	Wastewater surveillance of SARS-CoV-2 has become an attractive tool for combating the spread of COVID-19 by assessing the presence or levels of the virus shed in a population. However, the methods to quantify viral RNA and to link those quantities to the level of infection within the community vary. In this study, we sought to identify and optimize scalable methods for recovery of viral nucleic acids from wastewater and attempted to use a constitutive member of the gut virome, human-specific crAssphage, to help account for unknown levels of SARS-CoV-2 decay and dilution in the wastewater infrastructure. Results suggest that ultracentrifugation of a small volume of wastewater through a 50% sucrose cushion followed by total nucleic acid extraction yielded quantifiable virus in an area with a modest number of COVID-19 cases. Further, the ratio of log10(SARS-CoV-2):log10(crAssphage) appears to be associated with the cumulative incidence of COVID-19 in the Syracuse, NY area. In areas where ultracentrifuges are available, these methods may be used to link SARS-CoV-2 quantities in wastewater to levels of transmission within communities with sewer service.
has issue date	2020-05-23(xsd:dateTime)
bibo:doi	10.1101/2020.05.21.20109181
has license	medrxiv
sha1sum (hex)	2daf5bce6c08e45e6fccfeb43cac842ab44564c9
schema:url	https://doi.org/10.1101/2020.05.21.20109181
resource representing a document's title	Quantification of SARS-CoV-2 and cross-assembly phage (crAssphage) from wastewater to monitor coronavirus transmission within communities
resource representing a document's body	covid:2daf5bce6c08e45e6fccfeb43cac842ab44564c9#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/2daf5bce6c08e45e6fccfeb43cac842ab44564c9
is schema:about of	named entity 'account' named entity 'vary' named entity 'member' named entity 'wastewater' named entity 'COVID-19' named entity 'SURVEILLANCE' named entity 'NUCLEIC ACIDS' named entity 'LEVELS' named entity 'PRESENCE' named entity 'SPREAD' named entity 'INFECTION' named entity 'TO HELP' named entity 'VIRUS' named entity 'quantifiable' named entity 'SARS-CoV-2' named entity 'study' named entity 'link' named entity 'monitor' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'virome' named entity 'crAssphage' named entity 'virus' named entity 'COVID' named entity 'wastewater' named entity 'coronavirus' named entity 'crAssphage' named entity 'sucrose' named entity 'SARS-Cov-2' named entity 'SARS-CoV-2' named entity '75 minutes' named entity 'SARS-CoV-2' named entity 'peer review' named entity 'SARS-CoV-2' named entity 'CC-BY 4.0 International license' named entity 'nucleic acids' named entity 'social distancing' named entity 'ultracentrifugation' named entity 'viral particles' named entity 'crAssphage' named entity 'medRxiv' named entity 'Qiagen' named entity 'medRxiv' named entity 'qPCR' named entity 'cDNA' named entity 'medRxiv' named entity 'crAssphage' named entity 'ultracentrifugation' named entity 'aqueous' named entity 'micron' named entity 'nucleic acids' named entity 'medRxiv' named entity 'crAssphage' named entity 'quantitative analysis' named entity 'electronegative' named entity 't-tests' named entity 'NaCl' named entity 'public health' named entity 'May 6' named entity 'May 13' named entity 'standard curve' named entity 'SARS-CoV-2' named entity 'nucleic acids' named entity 'wastewater' named entity 'medRxiv' named entity 'wastewater' named entity 'RNA' named entity 'wastewater' named entity 'filtration' named entity 'ultracentrifugation' named entity 'SARS-CoV-2' named entity 'viral particles' named entity 'RNA' named entity 'CC-BY 4.0 International license' named entity 'Tris-HCl'

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