About: Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1

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About: Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1
Creator	Li, Shuang Yang, Haitao Zhao, Qi Chen, Cheng Wang, Fenghua Chen, Xia Fu, Sheng Tan, Yusheng Wang, Jinshan
Source	Medline; PMC
abstract	Human coronavirus HKU1 (HCoV-HKU1), which mainly causes acute self-limited respiratory-tract infections, belongs to group A of the Betacoronavirus genus. Coronavirus genomes encode 16 nonstructural proteins (nsp1–16), which assemble into a large replication–transcription complex mediating virus propagation. Nonstructural protein 9, which binds to the single-stranded DNA/RNA, has been shown to be indispensible for viral replication. Interestingly, a functional mutant (N60K) of nsp9 was identified to compensate for a 6 nt insertion mutation of the 3′-untranslated region (UTR), which is critical for viral RNA synthesis. It has been proposed that the N60K mutation may cause certain conformational changes of nsp9 to rescue the defective insertion mutant. To further investigate the underlying structural mechanism, the N60K mutant of nsp9 from HCoV-HKU1 was successfully crystallized in this study. The crystals diffracted to 2.6 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 31.9, b = 85.0, c = 95.0 Å. Two molecules were identified per asymmetric unit.
has issue date	2014-11-14(xsd:dateTime)
bibo:doi	10.1107/s2053230x14023085
bibo:pmid	25484211
has license	bronze-oa
sha1sum (hex)	2dd56fdde263e4c8b6a2e54123a89b858a1ecf46
schema:url	https://doi.org/10.1107/s2053230x14023085
resource representing a document's title	Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1
has PubMed Central identifier	PMC4259225
has PubMed identifier	25484211
schema:publication	Acta Crystallographica Section F Structural Biology Communications
resource representing a document's body	covid:2dd56fdde263e4c8b6a2e54123a89b858a1ecf46#body_text
is schema:about of	named entity 'insertion' named entity 'nonstructural proteins' named entity 'identified' named entity 'Coronavirus' named entity 'compensate' named entity 'STRUCTURAL BIOLOGY' named entity 'IDENTIFIED' named entity 'SINGLE-STRANDED DNA' named entity 'BETACORONAVIRUS' named entity 'CRITICAL' named entity 'CRYSTALS' named entity 'GENUS' named entity 'MEDIATING' named entity 'SPACE GROUP' named entity 'PROTEIN ' named entity 'NONSTRUCTURAL PROTEINS' named entity 'GROUP A' named entity 'LARGE' named entity 'ASSEMBLE' named entity 'STUDY' named entity 'BINDS' named entity 'UNTRANSLATED REGION' named entity 'PROPOSED' named entity 'CAUSE' named entity 'CRYSTALLIZED' named entity 'VIRAL REPLICATION' named entity 'MUTANT' named entity 'STRUCTURAL' named entity 'MUTATION' named entity 'ACUTE' named entity 'ENCODE' named entity 'UNIT' named entity 'VIRUS' named entity 'HUMAN CORONAVIRUS HKU1' named entity 'COMPENSATE' named entity 'FUNCTIONAL' named entity 'PROPAGATION' named entity 'REPLICATION' named entity 'RESCUE' named entity 'UNDERLYING' named entity '2.6' named entity 'INVESTIGATE' named entity 'COMMUNICATIONS' named entity 'MECHANISM' named entity 'CAUSES' named entity 'MOLECULES' named entity 'GROUP P2' named entity 'INSERTION MUTANT' named entity 'RNA SYNTHESIS' named entity 'VIRAL RNA' named entity 'TRANSCRIPTION' named entity 'GENOMES' named entity 'RESPIRATORY-TRACT' named entity 'ASYMMETRIC' named entity 'INSERTION MUTATION' named entity 'INFECTIONS' named entity 'DEFECTIVE' named entity 'PARAMETERS' named entity 'CORONAVIRUS' named entity 'CHANGES' named entity 'COMPLEX' named entity 'CELL' named entity 'CERTAIN' named entity 'RESOLUTION' named entity 'viral' named entity 'resolution' named entity 'insertion' named entity 'mutant' named entity 'binds' named entity 'untranslated region' named entity 'structural' named entity 'space group' named entity 'Structural Biology'

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