About: Hepatitis E Virus ORF2 Protein Activates the Pro-Apoptotic Gene CHOP and Anti-Apoptotic Heat Shock Proteins

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Hepatitis E Virus ORF2 Protein Activates the Pro-Apoptotic Gene CHOP and Anti-Apoptotic Heat Shock Proteins
Creator	John, Lijo Schaefer, Stephan Pü, Brigitte
source	Medline; PMC
abstract	BACKGROUND: Hepatitis E virus (HEV) is a non-enveloped plus-strand RNA virus that causes acute hepatitis. The capsid protein open reading frame 2 (ORF2) is known to induce endoplasmic reticulum stress in ORF2 expressing cells. METHODOLOGY/PRINCIPAL FINDINGS: In this study we found that HEV ORF2 activates the expression of the pro-apoptotic gene C/EBP homologous protein (CHOP). ORF2 stimulates the CHOP promoter mainly through AARE (amino acid response elements) and to a minor extent the ERSE (endoplasmic reticulum stress response elements). Activating transcription factor 4 (ATF4) protein binds and activates the AARE regulatory sites of the CHOP promoter. ORF2 expression also leads to increased phosphorylation of eukaryotic initiation factor 2 alpha (eIF2α) that in turn initiates the translation of ATF4 mRNA. The pro-apoptotic gene CHOP is an important trigger to initiate endoplasmic reticulum stress induced apoptosis. However, the activation of CHOP by ORF2 in this study did not induce apoptosis, nor did BCL2-associated X protein (Bax) translocate to mitochondria. Microarray analysis revealed an ORF2 specific increased expression of chaperones Hsp72, Hsp70B', and co-chaperone Hsp40. Co-immunoprecipitation (Co-IP) and in silico molecular docking analysis suggests that HEV ORF2 interacts with Hsp72. In addition, Hsp72 shows nuclear accumulation in ORF2 expressing cells. CONCLUSIONS/SIGNIFICANCE: These data provide new insight into simultaneously occurring counter-acting effects of HEV ORF2 that may be part of a strategy to prevent host suicide before completion of the viral replication cycle.
has issue date	2011-09-23(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0025378
bibo:pmid	21966512
has license	cc-by
sha1sum (hex)	2df184bde7ca6083d8a33a2b1ce05e302685dc72
schema:url	https://doi.org/10.1371/journal.pone.0025378
resource representing a document's title	Hepatitis E Virus ORF2 Protein Activates the Pro-Apoptotic Gene CHOP and Anti-Apoptotic Heat Shock Proteins
has PubMed Central identifier	PMC3179511
has PubMed identifier	21966512
schema:publication	PLoS One
resource representing a document's body	covid:2df184bde7ca6083d8a33a2b1ce05e302685dc72#body_text
is schema:about of	named entity 'Hepatitis E virus' named entity 'Virus' named entity 'ACTIVATES' named entity 'PRO-' named entity 'RNA VIRUS' named entity 'ORF2' named entity 'HEPATITIS E VIRUS' named entity 'PROTEIN ' named entity 'CAPSID PROTEIN' named entity 'BACKGROUND' named entity 'INDUCE' named entity 'CAUSES' named entity 'CELLS' named entity 'STRAND' named entity 'IS A' named entity 'OPEN READING FRAME' named entity 'CHOP' named entity 'HEPATITIS E VIRUS' named entity 'ORF2' named entity 'HEAT SHOCK PROTEINS' named entity 'GENE' named entity 'APOPTOTIC' named entity 'ACUTE HEPATITIS' named entity 'NON-' named entity 'PROTEIN ' named entity 'ENDOPLASMIC RETICULUM STRESS' named entity 'KNOWN' covid:arg/2df184bde7ca6083d8a33a2b1ce05e302685dc72 named entity 'acute hepatitis' named entity 'Apoptotic' named entity 'ORF2' named entity 'ORF2' named entity 'non-enveloped' named entity 'capsid protein' named entity 'open reading frame' named entity 'Hepatitis E Virus' named entity 'non-enveloped' named entity 'ORF2' named entity 'van der Waals force' named entity 'chaperone' named entity 'apoptosis' named entity 'anti-apoptotic' named entity 'binding sites' named entity 'ORF2' named entity 'pro-apoptotic' named entity 'diagnostic purposes' named entity 'protein' named entity 'H1299' named entity 'GRP78' named entity 'homologous recombination' named entity 'ligand' named entity 'Escherichia coli' named entity 'GFP' named entity 'contact energy' named entity 'HEV' named entity 'Germany' named entity 'sequence alignments' named entity 'Hsp72' named entity 'viruses' named entity 'cytoplasm' named entity 'heat shock' named entity 'HEV' named entity 'ORF2' named entity 'Hsp72' named entity 'ORF2' named entity 'family of proteins' named entity 'ATF4' named entity 'H1299' named entity 'gene promoter' named entity 'ORF2' named entity 'protein' named entity 'protein' named entity 'amino acids' named entity 'viral protein' named entity 'apoptosis' named entity 'Huh7' named entity 'co-chaperone' named entity 'Hsp72' named entity 'polyclonal antibody'

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