About: Cell Cycle-independent Role of Cyclin D3 in Host Restriction of Influenza Virus Infection

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About: Cell Cycle-independent Role of Cyclin D3 in Host Restriction of Influenza Virus Infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cell Cycle-independent Role of Cyclin D3 in Host Restriction of Influenza Virus Infection
Creator	Zhang, Yang Bruzzone, Roberto Kien, François Nal, Béatrice Fan, Ying Sanyal, Sumana Chan, Wai Chi, Michael Mok, Ka-Pun
Source	Medline; PMC
abstract	To identify new host factors that modulate the replication of influenza A virus, we performed a yeast two-hybrid screen using the cytoplasmic tail of matrix protein 2 from the highly pathogenic H5N1 strain. The screen revealed a high-score interaction with cyclin D3, a key regulator of cell cycle early G(1) phase. M2-cyclin D3 interaction was validated through GST pull-down and recapitulated in influenza A/WSN/33-infected cells. Knockdown of Ccnd3 by small interfering RNA significantly enhanced virus progeny titers in cell culture supernatants. Interestingly, the increase in virus production was due to cyclin D3 deficiency per se and not merely a consequence of cell cycle deregulation. A combined knockdown of Ccnd3 and Rb1, which rescued cell cycle progression into S phase, failed to normalize virus production. Infection by influenza A virus triggered redistribution of cyclin D3 from the nucleus to the cytoplasm, followed by its proteasomal degradation. When overexpressed in HEK 293T cells, cyclin D3 impaired binding of M2 with M1, which is essential for proper assembly of progeny virions, lending further support to its role as a putative restriction factor. Our study describes the identification and characterization of cyclin D3 as a novel interactor of influenza A virus M2 protein. We hypothesize that competitive inhibition of M1-M2 interaction by cyclin D3 impairs infectious virion formation and results in attenuated virus production. In addition, we provide mechanistic insights into the dynamic interplay of influenza virus with the host cell cycle machinery during infection.
has issue date	2017-03-24(xsd:dateTime)
bibo:doi	10.1074/jbc.m117.776112
bibo:pmid	28130444
has license	cc-by
sha1sum (hex)	2e34c8495ee4de8e0607b48585b97fa0c0da9dc9
schema:url	https://doi.org/10.1074/jbc.m117.776112
resource representing a document's title	Cell Cycle-independent Role of Cyclin D3 in Host Restriction of Influenza Virus Infection
has PubMed Central identifier	PMC5377818
has PubMed identifier	28130444
schema:publication	J Biol Chem
resource representing a document's body	covid:2e34c8495ee4de8e0607b48585b97fa0c0da9dc9#body_text
is schema:about of	named entity 'H5N1' named entity 'Restriction' covid:arg/2e34c8495ee4de8e0607b48585b97fa0c0da9dc9 named entity 'yeast two-hybrid screen' named entity 'modulate' named entity 'H5N1' named entity 'yeast two-hybrid screen' named entity 'Influenza Virus' named entity 'Dulbecco's modified Eagle's medium' named entity 'H5N1' named entity 'nucleus' named entity 'cyclins' named entity 'protein' named entity 'placenta' named entity 'nuclear material' named entity 'ISG' named entity 'IAV' named entity 'plasma membrane' named entity 'cell fractionation' named entity 'lamin B1' named entity 'cell culture' named entity 'cell cycle' named entity 'secretory pathway' named entity 'viral proteins' named entity 'cell cycle' named entity 'cyclin D3' named entity 'Transfection' named entity 'titer' named entity 'cyclin D3' named entity 'SARS-CoV' named entity 'transfected' named entity 'G 2' named entity 'cell cycle' named entity 'virus' named entity 'life cycle' named entity 'plasma membrane' named entity 'CDK4/6' named entity 'Sigma-Aldrich' named entity 'cyclin D3' named entity 'virus replication' named entity 'FITC' named entity 'proteasome' named entity 'diploids' named entity 'transfected' named entity 'viral infection' named entity '0.01' named entity 'dye' named entity 'steady-state levels' named entity 'Thermo Scientific' named entity 'MDCK' named entity 'plasmid' named entity 'mAb' named entity 'highly conserved' named entity 'IAV' named entity 'protein' named entity '0.01' named entity 'cell lysates' named entity 'siRNA' named entity 'expression profiles' named entity 'cyclin D3' named entity 'cyclin D3' named entity 'D-type' named entity 'IAV' named entity 'IAV' named entity 'protein' named entity 'cyclin D3' named entity 'Y2H' named entity 'virus' named entity 'life cycle' named entity 'CDK4/6' named entity 'wild-type' named entity 'virus' named entity 'virus'

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