About: Luteolin transforms the BMDM polarity to regulate the expression of inflammatory factors

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Luteolin transforms the BMDM polarity to regulate the expression of inflammatory factors Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Luteolin transforms the BMDM polarity to regulate the expression of inflammatory factors
Creator	Zhou, Jing Liu, Cao, Meng Mao, Xiaodong Wang, Shuxia Xu, Shuhang Yao, Xiaoming
Source	BioRxiv
abstract	Macrophage are indispensable regulator cells in inflammatory response. Macrophage polarization and its secreted inflammatory factors have affinity with the outcomes of inflammation. Luteolin, a flavonoid abundant in plants has anti-inflammatory activity, but whether luteolin can manipulate M1/M2 polarization of BMDM to suppress inflammation is still veiled. The purpose of this study was to observe the effects of luterolin on the polarity of BMDM derived from C57BL/6 mice and the expression of inflammatory factors, to explore the mechanism of luteolin regulating the BMDM polarity. M1-polarized BMDM were induced by LPS+IFN-γ, M2-polarization were stimulated with IL-4. BMDM morphology was observed by laser confocal microscopy; levels of BMDM differentiation and CD11c or CD206 on membrane surface were assessed by FCM; mRNA and protein of M1/M2-type inflammatory factors were performed by qPCR and ELISA, respectively; the expression of p-STAT1 and p-STAT6 protein pathways was detected by Western-blotting. The isolated mouse bone marrow cells were successfully differentiated into BMDM, LPS+IFN-γ induced BMDM M1-phenotype polarization, and IL-4 induced its M2-phenotype polarization. After M1-polarized BMDM treated with luteolin, M1-type pro-inflammatory factors including IL-6, TNF-α□iNOS, CD86 were down-regulated while M2-type anti-inflammatory factors including IL-10, Arg1, CD206 were up-regulated; the expression of M1-type surface marker CD11c decreased, nevertheless, M2-type marker CD206 increased; levels of inflammatory signaling protein p-STAT1 and p-STAT6 were attenuated and enhanced respectively. Our study suggests luteolin may transform BMDM polarity through p-STAT1/6 to regulate the expression of inflammatory mediators, thereby inhibiting inflammation. Naturally occurring luteolin hold promise as an anti-inflammatory and immunomodulatory agent.
has issue date	2020-07-02(xsd:dateTime)
bibo:doi	10.1101/2020.06.30.181503
has license	biorxiv
sha1sum (hex)	2e4779421f75f5363ddccd9ab28cace181c01ce6
schema:url	https://doi.org/10.1101/2020.06.30.181503
resource representing a document's title	Luteolin transforms the BMDM polarity to regulate the expression of inflammatory factors
schema:publication	bioRxiv
resource representing a document's body	covid:2e4779421f75f5363ddccd9ab28cace181c01ce6#body_text
is schema:about of	named entity 'anti-inflammatory' named entity 'inflammation' named entity 'Macrophage' named entity 'inflammation' named entity 'Macrophage polarization' named entity 'inflammatory factors' named entity 'luteolin' named entity 'STAT6' named entity 'COVID-19' named entity 'ng/mL' named entity 'STAT1' named entity 'RNA' named entity 'IL-4' named entity 'angiogenesis' named entity 'luteolin' named entity 'luteolin' named entity 'Arg1' named entity 'macrophage polarization' named entity 'serum' named entity 'immune response' named entity 'STAT6' named entity 'viral infection' named entity 'Physalin' named entity 'IL-6' named entity 'Primer' named entity 'Metabolism' named entity 'IFN' named entity 'infectious agents' named entity 'anti-inflammatory' named entity 'mannose receptor' named entity 'inflammation' named entity 'pro-inflammatory cytokines' named entity 'IL-4' named entity 'pro-inflammatory' named entity 'macrophages' named entity 'macrophages' named entity 'morphology' named entity 'Immunoreactive' named entity 'STAT1' named entity 'qPCR' named entity 'IL-10' named entity 'bacterial infection' named entity 'IL-4' named entity 'anti-inflammatory' named entity 'STAT1' named entity 'arginine' named entity 'infectious disease' named entity 'anti-inflammatory' named entity 'iNOS' named entity 'M2 macrophage' named entity 'repolarize' named entity 'incubated' named entity 'polarization' named entity 'cytokines' named entity 'macrophage' named entity 'luteolin' named entity 'assay' named entity 'microscope' named entity 'anti-inflammatory' named entity 'macrophages' named entity 'culture medium' named entity 'receptor' named entity 'iNOS' named entity 'cytokine storm' named entity 'LPS' named entity 'qPCR' named entity 'down-regulated' named entity 'pathogens' named entity 'room temperature' named entity 'inflammatory factors' named entity 'membrane surface' named entity 'inflammatory factors' named entity 'polarization' named entity 'luteolin' named entity 'polarization' named entity 'luteolin' named entity 'statistical difference' named entity 'Shanghai'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software