About: Detection of avian infectious bronchitis viral infection using in situ hybridization and recombinant DNA

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About: Detection of avian infectious bronchitis viral infection using in situ hybridization and recombinant DNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of avian infectious bronchitis viral infection using in situ hybridization and recombinant DNA
Creator	Wang, Li Collisson, Ellen Sneed, Loyd Li, Junzhou Peters, Mary
Source	PMC
abstract	A recombinant DNA probe with specificity for the 3′ end of genomic RNA from the Ark 99 strain of infectious bronchitis virus (IBV) was found to hybridize with extracted RNA of three strains with the Ark serotype, as well as the Mass41, Holl52, Gray, JMK, Conn, Fla and SE17 strains of IBV. Viral infection was detected in the cytoplasm of chicken embryo kidney cells inoculated with Mass41, Ark99, SE17 or two recent field isolates of IBV using in situ cytohybridization and a biotinylated probe. In vivo infections were detected in individual cells of tracheas and lungs 2, 4, and 6 days after inoculation of chicks with Mass41 and Ark99. In situ hybridization of Ark99 infected tissue sections using (32)P-dATP labelled probe indicated that more viral replication was present in the trachea on day 4 than either days 2 or 6; whereas more viral RNA was found in the lungs on day 6 than days 2 or 4 after inoculation.
has issue date	2002-11-13(xsd:dateTime)
bibo:doi	10.1016/0378-1135(90)90176-v
bibo:pmid	2175524
has license	no-cc
sha1sum (hex)	2f2f4e48566c241d4cc44d404d8cb7f381d77bdd
schema:url	https://doi.org/10.1016/0378-1135%2890%2990176-v
resource representing a document's title	Detection of avian infectious bronchitis viral infection using in situ hybridization and recombinant DNA
has PubMed Central identifier	PMC7117324
has PubMed identifier	2175524
schema:publication	Vet Microbiol
resource representing a document's body	covid:2f2f4e48566c241d4cc44d404d8cb7f381d77bdd#body_text
is schema:about of	named entity 'AVIAN INFECTIOUS BRONCHITIS' named entity 'BRONCHITIS VIRAL' named entity 'hybridization' named entity 'VIRAL INFECTION' named entity 'USING' named entity 'DETECTION' named entity 'BRONCHITIS VIRAL' named entity 'VIRAL INFECTION' named entity 'IN SITU HYBRIDIZATION' named entity 'RECOMBINANT DNA' named entity 'DETECTION' named entity 'WANG' named entity 'IN SITU HYBRIDIZATION' named entity 'AVIAN INFECTIOUS BRONCHITIS' named entity 'VET' named entity 'RECOMBINANT DNA' named entity 'USING' covid:arg/2f2f4e48566c241d4cc44d404d8cb7f381d77bdd named entity 'Wang' named entity 'Sneed' named entity 'hybridization' named entity 'recombinant DNA' named entity 'viral infection' named entity 'avian infectious bronchitis' named entity 'Vet' named entity 'IBV' named entity 'hydrogen peroxide' named entity 'SE17' named entity 'viral pathogenesis' named entity 'cell culture' named entity 'autoradiographs' named entity 'IBV' named entity 'trachea' named entity 'glacial acetic acid' named entity 'hybridization' named entity 'SDS' named entity 'nuclei' named entity 'IBV' named entity 'Virus' named entity 'viral replication' named entity 'sodium acetate' named entity 'highly contagious' named entity 'cartilage' named entity 'allantoic' named entity 'RNA' named entity 'hybridization' named entity 'viral replication' named entity 'biotinylated' named entity 'Viral replication' named entity 'chicken eggs' named entity 'lungs' named entity 'plasmid' named entity 'Western blot' named entity 'University of Delaware' named entity 'viral RNA' named entity 'trachea' named entity 'bluetongue' named entity 'Virus' named entity 'hybridization' named entity 'NDV' named entity 'dATP' named entity 'lungs' named entity 'allantoic' named entity 'cartilage' named entity 'cultured cells' named entity 'hybridization' named entity 'University of Delaware' named entity 'streptavidin' named entity '3' end' named entity 'Richmond, CA' named entity 'lungs' named entity 'trachea' named entity 'lungs' named entity 'cecal' named entity 'viral RNA' named entity 'methyl cellulose' named entity 'virus'

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