About: Dudleya brittonii extract promotes survival rate and M2-like metabolic change in porcine 3D4/31 alveolar macrophages

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About: Dudleya brittonii extract promotes survival rate and M2-like metabolic change in porcine 3D4/31 alveolar macrophages Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Dudleya brittonii extract promotes survival rate and M2-like metabolic change in porcine 3D4/31 alveolar macrophages
Creator	Kim, Hyungkuen Kim, Sung-Jo Park, Byung-Chul Hyung Jeon, Eek
Source	Medline; PMC
abstract	OBJECTIVE: Although alveolar macrophages play a key role in the respiratory immunity of livestock, studies on the mechanism of differentiation and survival of alveolar macrophages are lacking. Therefore, we undertook to investigate changes in the lipid metabolism and survival rate, using 3D4/31 macrophages and Dudleya brittonii which has been used as a traditional asthma treatment. METHODS: 3D4/31 macrophages were used as the in vitro porcine alveolar macrophages model. The cells were activated by exposure to phorbol 12-myristate 13-acetate (PMA). Dudleya brittonii extraction was performed with distilled water. For evaluating the cell survival rate, we performed the water-soluble tetrazolium salt cell viability assay and growth curve analysis. To confirm cell death, cell cycle and intracellular reactive oxygen species (ROS) levels were measured using flow cytometric analysis by applying fluorescence dye dichlorofluorescein diacetate and propidium iodide. Furthermore, we also evaluated cellular lipid accumulation with oil red O staining, and fatty acid synthesis related genes expression levels using quantitative polymerase chain reaction (qPCR) with SYBR green dye. Glycolysis, fatty acid oxidation, and tricarboxylic acid (TCA) cycle related gene expression levels were measured using qPCR after exposure to Dudleya brittonii extract (DB) for 12 h. RESULTS: The ROS production and cell death were induced by PMA treatment, and exposure to DB reduced the PMA induced downregulation of cell survival. The PMA and DB treatments upregulated the lipid accumulation, with corresponding increase in the acetyl-CoA carboxylase alpha, fatty acid synthase mRNA expressions. DB-PMA co-treatment reduced the glycolysis genes expression, but increased the expressions of fatty acid oxidation and TCA cycle genes. CONCLUSION: This study provides new insights and directions for further research relating to the immunity of porcine respiratory system, by employing a model based on alveolar macrophages and natural materials.
has issue date	2019-05-27(xsd:dateTime)
bibo:doi	10.5713/ajas.19.0251
bibo:pmid	31208190
has license	cc-by
sha1sum (hex)	2f314a77ea26734587af46d6ed32345b0160760e
schema:url	https://doi.org/10.5713/ajas.19.0251
resource representing a document's title	Dudleya brittonii extract promotes survival rate and M2-like metabolic change in porcine 3D4/31 alveolar macrophages
has PubMed Central identifier	PMC6817779
has PubMed identifier	31208190
schema:publication	Asian-Australas J Anim Sci
resource representing a document's body	covid:2f314a77ea26734587af46d6ed32345b0160760e#body_text
is schema:about of	named entity 'Dudleya' named entity 'PMA' named entity 'levels' named entity 'intracellular' named entity 'reactive oxygen species (ROS)' named entity 'porcine' named entity 'Dudleya' named entity 'alveolar' named entity 'METABOLIC CHANGE' named entity 'REACTIVE OXYGEN SPECIES' named entity 'QPCR' named entity 'USED' named entity 'WATER-SOLUBLE' named entity 'LEVELS' named entity 'CELLULAR' named entity 'DYE' named entity 'GENE EXPRESSION LEVELS' named entity 'MACROPHAGES' named entity 'MECHANISM' named entity 'CELL CYCLE' named entity 'TETRAZOLIUM SALT' named entity 'LACKING' named entity 'CELL SURVIVAL RATE' named entity 'GLYCOLYSIS' named entity 'CONFIRM' named entity 'FATTY ACID SYNTHESIS' named entity 'CELL DEATH' named entity 'GREEN ' named entity 'FLUORESCENCE' named entity 'PORCINE' named entity 'OIL RED O' named entity '3D4' named entity 'QUANTITATIVE POLYMERASE CHAIN REACTION' named entity 'IMMUNITY' named entity 'DUDLEYA BRITTONII' named entity 'ALVEOLAR MACROPHAGES' named entity 'TRICARBOXYLIC ACID' named entity 'PHORBOL 12-MYRISTATE 13-ACETATE' named entity 'INTRACELLULAR' named entity 'RELATED' named entity 'OBJECTIVE' named entity 'MEASURED' named entity 'CYCLE' named entity 'CHANGES' named entity 'DISTILLED WATER' named entity 'LIPID METABOLISM' named entity 'CELL VIABILITY ASSAY' named entity 'SURVIVAL' named entity 'CHANGE IN' named entity 'TREATMENT' named entity 'PERFORMED' named entity 'IN VITRO' named entity 'SYBR GREEN' named entity 'USING' named entity 'LIKE' named entity 'PLAY' named entity 'FATTY ACID OXIDATION' named entity 'PROPIDIUM IODIDE' named entity 'METABOLISM ' named entity 'ALVEOLAR MACROPHAGES' named entity 'EXTRACT' named entity 'LIPID ACCUMULATION' named entity 'STAINING' named entity 'DUDLEYA BRITTONII' named entity '3D4' named entity 'PORCINE' named entity 'CELLS' named entity 'TO INVESTIGATE' named entity 'METHODS' named entity 'EVALUATED' named entity 'ASTHMA' named entity 'EXPOSURE TO' named entity 'EVALUATING' named entity 'APPLYING' named entity 'STUDIES' named entity 'DICHLOROFLUORESCEIN DIACETATE' named entity 'SURVIVAL RATE' named entity 'EXTRACTION'

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