About: Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats

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About: Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats
Creator	Zhao, Lei Guo, Huifen Guo, Xiao Zhang, Jian-Xu Zhang, Yao-Hua
Source	Medline; PMC
abstract	BACKGROUND: In rats, urine-borne male pheromones comprise organic volatile compounds and major urinary proteins (MUPs). A number of volatile pheromones have been reported, but no MUP pheromones have been identified in rat urine. RESULTS: We used sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing electrophoresis (IEF), nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) after in gel digestion of the proteins and quantitative real-time PCR (qRT-PCR) and showed that the levels of two MUPs, odorant-binding protein 3 (OBP3) (i.e. PGCL4) and MUP13 (i.e. PGCL1), in urine and their mRNAs in liver were higher in males than in females and were suppressed by orchidectomy and restored by testosterone treatment (T treatment). We then generated recombinant MUPs (rMUPs) and found that the sexual attractiveness of urine from castrated males to females significantly increased after the addition of either recombinant OBP3 (rOBP3) or recombinant MUP13 (rMUP13). Using c-Fos immunohistochemistry, we further examined neuronal activation in the brains of female rats after they sniffed rOBP3 or rMUP13. Both rOBP3 and rMUP13 activated the accessory olfactory bulb (AOB), medial preoptic area (MPA), bed nucleus of the stria terminalis (BST), medial amygdala (MeA), posteromedial cortical amygdala (PMCo) and ventromedial nucleus of the hypothalamus (VMH), which participate in the neural circuits responsible for pheromone-induced sexual behaviours. In particular, more c-Fos-immunopositive (c-Fos-ir) cells were observed in the posterior AOB than in the anterior AOB. CONCLUSIONS: The expression of OBP3 and MUP13 was male-biased and androgen-dependent. They attracted females and activated brain areas related to sexual behaviours in female rats, suggesting that both OBP3 and MUP13 are male pheromones in rats. Particularly, an OBP excreted into urine was exemplified to be a chemical signal.
has issue date	2018-02-23(xsd:dateTime)
bibo:doi	10.1186/s12983-018-0254-0
bibo:pmid	29483934
has license	cc-by
sha1sum (hex)	3053529090a8974edc1ad695d3be4d407eef9674
schema:url	https://doi.org/10.1186/s12983-018-0254-0
resource representing a document's title	Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats
has PubMed Central identifier	PMC5824612
has PubMed identifier	29483934
schema:publication	Front Zool
resource representing a document's body	covid:3053529090a8974edc1ad695d3be4d407eef9674#body_text
is schema:about of	named entity 'PREDOMINANT' named entity 'OBP3' named entity 'volatile compounds' named entity 'urine' named entity 'pheromones' covid:arg/3053529090a8974edc1ad695d3be4d407eef9674 named entity 'PHEROMONES' named entity 'MAJOR URINARY PROTEINS' named entity 'BUT' named entity 'HAVE' named entity 'IDENTIFIED' named entity 'MUP' named entity 'NUMBER OF' named entity 'REPORTED' named entity 'PHEROMONES' named entity 'COMPRISE' named entity 'VOLATILE' named entity 'ORGANIC' named entity 'URINE' named entity 'RATS' named entity 'MALE' named entity 'MUP13' named entity 'MUPS' named entity 'MALE' named entity 'RAT' named entity 'BORNE' named entity 'RATS' named entity 'BACKGROUND' named entity 'male' named entity 'volatile compounds' named entity 'pheromones' named entity 'urine' named entity 'MUPs' named entity 'MUP' named entity 'Bio-Rad' named entity 'MUPs' named entity 'epigenetic' named entity 'lipocalin' named entity 'sham-operated' named entity 'PBS' named entity 'sexual attraction' named entity 'trifluoroacetic acid' named entity 'recombinant proteins' named entity 'amino acid residue' named entity 'c-Fos' named entity 'acetonitrile' named entity '0.01' named entity 'Bio-Rad' named entity 'urine' named entity 'MUP' named entity 'liver' named entity 'Alpha-2u globulin' named entity 'ncbi' named entity 'tear glands' named entity 'One-way ANOVA' named entity 'pheromones' named entity 't-test' named entity 'Laboratory Animal' named entity 'MUP' named entity 'immunohistochemistry' named entity 'centrifugation' named entity 'amino acids' named entity 'denaturation' named entity 'kDa' named entity 'Bradford protein assay' named entity 'kDa' named entity 'castrated' named entity 'sucrose' named entity 'liver' named entity 'ice box' named entity 'urine' named entity 'urine' named entity 'urine' named entity 'outbred' named entity 'La Jolla' named entity 'sonication' named entity 'CBB'

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