About: Tear lipocalin is the major endonuclease in tears

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About: Tear lipocalin is the major endonuclease in tears Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Tear lipocalin is the major endonuclease in tears
Creator	Narsinh, Kiran Abduragimov, Adil Gasymov, Oktay Glasgow, Ben Yusifov, Taleh
Source	PMC
abstract	PURPOSE: Human endonucleases are integral to apoptosis in which unwanted or potentially harmful cells are eliminated. The rapid turnover of ocular surface epithelium and microbial colonization of the eyelids are continual sources of DNA in tears. Here, we determine the principal sources of endonuclease activity in tears. METHODS: Endonucleases in human tears were identified after Sephadex G100 gel filtration. DNA hydrolyzing activity was measured by the conversion pUC19 plasmid DNA to its circular form in agarose gels. Fractions with endonuclease activity were further isolated using a combination ConA-Sepharose DNA, oligo (dT) cellulose, and anion exchange chromatographies. The molecular weights of the DNA hydrolyzing proteins were estimated in zymograms and by calibration of size exclusion chromatography. DNase activities were characterized for activity at a variety of pH and ion concentrations as well as in the presence of inhibitors including NiCl(2), ZnCl(2), G-actin, and aurintricarboxylic acid (ATA). To determine the mode of hydrolysis, the cleaved ends of the DNA digested by tear DNases were analyzed by 3′ and 5′ end labeling using either terminal deoxynucleotidyl transferase or polynucleotide kinase with or without pretreatment with alkaline phosphatase. RESULTS: Tear lipocalin (TL) accounts for over 75% of the DNA catalytic activity in tears while a second endonuclease, ~34 kDa, is responsible for less than 24% of the activity. Both are Mg(2+) dependent enzyme endonucleases that are enhanced by Ca(2+), active at physiologic pH, inhibited by aurintricarboxylic acid, and catalyze hydrolysis of DNA to produce 3′-OH/5′P ends. However, the two enzymes can be distinguished by the inhibitory effect of NiCl(2) and the sizes of the cleaved DNA fragments. CONCLUSIONS: Two magnesium dependent extracellular endonucleases were identified in tears that are different from other major human extracellular nucleases. TL is the principal endonuclease in human tear fluid. Tear endonucleases have unique characteristics that differ from other known human endonucleases.
has issue date	2008-01-29(xsd:dateTime)
bibo:pmid	18334931
has license	cc-by
sha1sum (hex)	308e91d0c4575362a0d169b9ed31c60f79a86437
resource representing a document's title	Tear lipocalin is the major endonuclease in tears
has PubMed Central identifier	PMC2254967
has PubMed identifier	18334931
schema:publication	Mol Vis
resource representing a document's body	covid:308e91d0c4575362a0d169b9ed31c60f79a86437#body_text
is schema:about of	named entity 'ENDONUCLEASE' named entity 'MAJOR' named entity 'identified' named entity 'tears' named entity 'size exclusion chromatography' named entity 'ION' named entity 'G-ACTIN' named entity 'SURFACE EPITHELIUM' named entity 'APOPTOSIS' named entity 'FORM' named entity 'RAPID' named entity 'HARMFUL' named entity 'CONTINUAL' named entity 'SEPHADEX' named entity 'ACTIVITIES' named entity 'PURPOSE' named entity 'GELS' named entity 'ISOLATED' named entity 'ENDONUCLEASES' named entity 'AURINTRICARBOXYLIC ACID' named entity 'FRACTIONS' named entity 'OLIGO' named entity 'OCULAR SURFACE' named entity 'CONA' named entity 'CELLULOSE' named entity 'G100' named entity 'NICL2' named entity 'TURNOVER' named entity 'CHROMATOGRAPHIES' named entity 'ENDONUCLEASE ACTIVITY' named entity 'MOLECULAR WEIGHTS' named entity 'ESTIMATED' named entity 'CELLS' named entity 'AGAROSE' named entity 'VARIETY' named entity 'DETERMINE' named entity 'SEPHAROSE' named entity 'IDENTIFIED' named entity 'INTEGRAL' named entity 'CHARACTERIZED' named entity 'ACTIVITY' named entity 'MEASURED' named entity 'PROTEINS' named entity 'INCLUDING' named entity 'GEL FILTRATION' named entity 'CIRCULAR' named entity 'COMBINATION' named entity 'TEARS' named entity 'SOURCES' named entity 'EYELIDS' named entity 'CONCENTRATIONS' named entity 'PRINCIPAL' named entity 'ZNCL2' named entity 'ITS' named entity 'CALIBRATION' named entity 'ELIMINATED' named entity 'PLASMID DNA' named entity 'HUMAN' named entity 'TEAR LIPOCALIN' named entity 'INHIBITORS' named entity 'DNASE' named entity 'SIZE EXCLUSION CHROMATOGRAPHY' named entity 'CONVERSION' named entity 'USING' named entity 'PRESENCE OF' named entity 'TEARS' named entity 'MICROBIAL COLONIZATION' named entity 'DNA' named entity 'HERE' named entity 'EXCHANGE' named entity 'ANION' named entity 'METHODS' covid:arg/308e91d0c4575362a0d169b9ed31c60f79a86437 named entity 'estimated' named entity 'anion' named entity 'surface' named entity 'activity' named entity 'harmful' named entity 'cellulose'

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