About: An in vivo system for directed experimental evolution of rabbit haemorrhagic disease virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	An in vivo system for directed experimental evolution of rabbit haemorrhagic disease virus
Creator	Strive, Tanja Capucci, Lorenzo Esposito, Simona Frese, Michael Matthaei, Markus Kerr, Peter Hall, Robyn
source	PMC
abstract	The calicivirus Rabbit haemorrhagic disease virus (RHDV) is widely used in Australia as a biocontrol agent to manage wild European rabbit (Oryctolagus cuniculus) populations. However, widespread herd immunity limits the effectiveness of the currently used strain, CAPM V-351. To overcome this, we developed an experimental platform for the selection and characterisation of novel RHDV strains. As RHDV does not replicate in cell culture, variant viruses were selected by serially passaging a highly virulent RHDV field isolate in immunologically naïve laboratory rabbits that were passively immunised 18–24 hours post-challenge with a neutralising monoclonal antibody. After seven passages, two amino acid substitutions in the P2 domain of the capsid protein became fixed within the virus population. Furthermore, a synonymous substitution within the coding sequence of the viral polymerase appeared and was also maintained in all subsequent passages. These findings demonstrate proof-of-concept that RHDV evolution can be experimentally manipulated to select for virus variants with altered phenotypes, in this case partial immune escape.
has issue date	2017-03-13(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0173727
bibo:pmid	28288206
has license	cc-by
sha1sum (hex)	31fabefa068bca926a43f94ef80df1daf41aa806
schema:url	https://doi.org/10.1371/journal.pone.0173727
resource representing a document's title	An in vivo system for directed experimental evolution of rabbit haemorrhagic disease virus
has PubMed Central identifier	PMC5348035
has PubMed identifier	28288206
schema:publication	PLoS One
resource representing a document's body	covid:31fabefa068bca926a43f94ef80df1daf41aa806#body_text
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