About: A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan™ technology

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About: A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan™ technology Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan™ technology
Creator	Smith, Jemma Black, Elizabeth Heaton, Paul Lowings, J Mcelhinney, Lorraine
Source	Elsevier; Medline; PMC
abstract	A rapid and sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay incorporating TaqMan™ probes has been developed that can distinguish among the six established rabies and rabies-related virus genotypes. TaqMan™ probes were designed and validated against 106 rabies and rabies-related virus isolates, one isolate of the Australian bat Lyssaviruses (genotype 7), and 18 other non-rabies viruses important in the veterinary field. The N gene was used as the target for the probes as it is well conserved and has been intensively used to genotype rabies isolates. Additionally, it was found to contain regions specific to each genotype conducive to probe design. The RT-PCR assay described amplifies a portion of the nucleoprotein gene of all 107 rabies and rabies-related viruses, but none of the other viruses tested. Inclusion of TaqMan™-genotype-specific probes in the RT-PCR assay permits rapid identification of the virus present. By combining RT-PCR with TaqMan™ genotyping probes suspect rabies virus isolates can be identified in a single closed tube system that prevents potential PCR-product carry over contamination.
has issue date	2002-05-14(xsd:dateTime)
bibo:doi	10.1016/s0166-0934(02)00062-9
bibo:pmid	12176139
has license	no-cc
sha1sum (hex)	32fa36ff103ee2bf977160e78f3c7e5186375f3f
schema:url	https://doi.org/10.1016/s0166-0934%2802%2900062-9
resource representing a document's title	A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan™ technology
has PubMed Central identifier	PMC7119615
has PubMed identifier	12176139
schema:publication	J Virol Methods
resource representing a document's body	covid:32fa36ff103ee2bf977160e78f3c7e5186375f3f#body_text
is schema:about of	named entity 'TECHNOLOGY' named entity 'viruses' named entity 'RT-PCR' named entity 'viruses' named entity 'rabies' named entity 'genotyping' named entity 'genotypes' named entity 'combining' named entity 'TaqMan' named entity 'GENOTYPES' named entity 'TAQMAN' named entity 'AUSTRALIAN' named entity 'REGIONS' named entity 'RAPID' named entity 'N GENE' named entity 'GENOTYPES' named entity 'CARRY' named entity 'RABIES VIRUSES' named entity 'PRODUCT' named entity 'SYSTEM' named entity 'SPECIFIC' named entity 'GENOTYPING' named entity 'GENE' named entity 'POTENTIAL' named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION' named entity 'RT-PCR ASSAY' named entity 'ESTABLISHED' named entity 'ESTABLISHED' named entity 'VETERINARY' named entity 'DIFFERENTIATE' named entity 'RELATED' named entity 'IDENTIFICATION' named entity 'NUCLEOPROTEIN' named entity 'RABIES VIRUS' named entity 'RABIES' named entity 'PREVENTS' named entity 'DESCRIBED' named entity 'RELATED' named entity 'IMPORTANT' named entity 'TO CONTAIN' named entity 'TESTED' named entity 'VIRUS PRESENT' named entity 'VALIDATED' named entity 'FOUND' named entity 'USED' named entity 'PERMITS' named entity 'BUT' named entity 'CLOSED' named entity 'FIELD' named entity 'SUSPECT' named entity 'SENSITIVE' named entity 'ASSAY' named entity 'BAT' named entity 'RT-PCR' named entity 'TAQMAN' named entity '107' named entity 'PCR' named entity 'CONTAMINATION' named entity 'ISOLATES' named entity 'GENOTYPE' named entity 'NON-' named entity 'IDENTIFIED' named entity 'RABIES' named entity 'TUBE' named entity 'COMBINING' named entity 'SINGLE' named entity 'INCLUSION' named entity 'ISOLATE' named entity 'VIRUSES' named entity 'PORTION' named entity 'VIRUSES' named entity 'RAPID' named entity 'USING' named entity 'TARGET' named entity 'CONSERVED' named entity 'VIRUS' named entity 'PROBES'

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