About: Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model

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About: Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model
Creator	Devlin, Joanne Hewson, Kylie Ae, H Ae, Mardani Amir, A Karim, A
Source	Medline; PMC
abstract	Infectious bronchitis virus (IBV) is a coronavirus that causes upper respiratory, renal and/or reproductive diseases with high morbidity in poultry. Classification of IBV is important for implementation of vaccination strategies to control the disease in commercial poultry. Currently, the lengthy process of sequence analysis of the IBV S1 gene is considered the gold standard for IBV strain identification, with a high nucleotide identity (e.g. ≥95%) indicating related strains. However, this gene has a high propensity to mutate and/or undergo recombination, and alone it may not be reliable for strain identification. A real-time polymerase chain reaction (RT-PCR) combined with high-resolution melt (HRM) curve analysis was developed based on the 3′UTR of IBV for rapid detection and classification of IBV from commercial poultry. HRM curves generated from 230 to 435-bp PCR products of several IBV strains were subjected to further analysis using a mathematical model also developed during this study. It was shown that a combination of HRM curve analysis and the mathematical model could reliably group 189 out of 190 comparisons of pairs of IBV strains in accordance with their 3′UTR and S1 gene identities. The newly developed RT-PCR/HRM curve analysis model could detect and rapidly identify novel and vaccine-related IBV strains, as confirmed by S1 gene and 3′UTR nucleotide sequences. This model is a rapid, reliable, accurate and non-subjective system for detection of IBVs in poultry flocks.
has issue date	2009-03-20(xsd:dateTime)
bibo:doi	10.1007/s00705-009-0357-1
bibo:pmid	19301093
has license	no-cc
sha1sum (hex)	341192a37fe4b204f5c268b4f703db8688fde590
schema:url	https://doi.org/10.1007/s00705-009-0357-1
resource representing a document's title	Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model
has PubMed Central identifier	PMC7086978
has PubMed identifier	19301093
schema:publication	Arch Virol
resource representing a document's body	covid:341192a37fe4b204f5c268b4f703db8688fde590#body_text
is schema:about of	named entity 'DETECTION' named entity 'IBV' named entity 'melt' named entity 'HRM' named entity 'IBV' named entity 'RT-PCR' named entity 'rapidly' named entity 'identification' named entity 'nucleotide' named entity 'process' named entity 'pairs' named entity 'gene' named entity 'Rapid' named entity 'ACCURATE' named entity 'RT-PCR' named entity 'REPRODUCTIVE' named entity 'VACCINATION' named entity 'CONFIRMED BY' named entity 'RAPIDLY' named entity 'TO CONTROL' named entity '190' named entity 'RELATED' named entity 'IMPLEMENTATION' named entity 'SUBJECTIVE' named entity 'MUTATE' named entity 'IDENTIFICATION' named entity 'GROUP' named entity 'MELT' named entity 'DISEASE' named entity 'IMPORTANT' named entity 'MORBIDITY' named entity 'PCR' named entity 'PROCESS' named entity 'RESPIRATORY' named entity 'C95' named entity 'RELIABLE' named entity 'GENERATED' named entity 'CONSIDERED' named entity 'NON-' named entity '189' named entity 'RECOMBINATION' named entity 'STRAINS' named entity 'GENE' named entity 'RENAL' named entity 'HIGH-RESOLUTION' named entity 'MATHEMATICAL MODEL' named entity 'IN ACCORDANCE WITH' named entity 'MELT CURVE ANALYSIS' named entity 'POULTRY' named entity 'DETECT' named entity 'HIGH' named entity 'REAL-TIME POLYMERASE CHAIN REACTION' named entity 'CURRENTLY' named entity 'MODEL' named entity 'MATHEMATICAL MODEL' named entity 'SUBJECTIVE' named entity 'USING' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'NON-' named entity 'THEIR' named entity 'PAIRS' named entity 'ANALYSIS' named entity 'CORONAVIRUS' named entity 'CURVE' named entity 'ISOLATES' named entity 'HIGH-RESOLUTION' named entity 'PRODUCTS' named entity 'NUCLEOTIDE SEQUENCES' named entity 'RAPID' named entity 'GOLD STANDARD' named entity 'STRAIN' named entity 'SYSTEM' named entity 'S1 GENE' named entity 'IDENTIFY' named entity 'SEQUENCE ANALYSIS' named entity 'UPPER'

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