About: Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk
Creator	Enjuanes, Luis Sola, Isabel Castilla, Joaquin Pintado', Belen Sanchez-Morgado, Jose
Source	Medline; PMC
abstract	Protection against enteric infections can be provided by the oral administration of pathogen-neutralizing antibodies. To provide passive immunity, 18 lines of transgenic mice secreting a recombinant monoclonal antibody (Mab) neutralizing transmissible gastroenteritis coronavirus (TGEV) into the milk were generated. The genes encoding a chimeric Mab with the variable modules of the murine TGEV-specific Mab 6A.C3 and the constant modules of a human IgG(1), isotype Mab were expressed under the control of regulatory sequences derived from the whey acidic protein, which is an abundant milk protein. The Mab 6A.C3 binds to a highly conserved epitope present in coronaviruses of several species, which does not allow the selection of neutralization escape mutants. Antibody expression titers of 10(6) were obtained in the milk of transgenic mice that reduced TGEV infectivity 10(6)-fold. The antibody was synthesized at high levels throughout lactation. Integration of matrix attachment region sequences with the antibody genes led to a 20- to 10,000-fold increase in the antibody titer in 50% of the transgenic animals. Antibody expression levels were transgene copy number independent and related to the site of integration. The generation of transgenic animals producing virus neutralizing antibodies in milk could provide an approach to protection against neonatal infections of the enteric tract.
has issue date	1998-01-01(xsd:dateTime)
bibo:doi	10.1038/nbt0498-349
bibo:pmid	9555725
has license	no-cc
sha1sum (hex)	34ba544a9eb8cda4ea4be83e1a351e8638067f01
schema:url	https://doi.org/10.1038/nbt0498-349
resource representing a document's title	Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk
has PubMed Central identifier	PMC7097410
has PubMed identifier	9555725
schema:publication	Nat Biotechnol
resource representing a document's body	covid:34ba544a9eb8cda4ea4be83e1a351e8638067f01#body_text
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