About: Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin

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About: Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin
Creator	Wu, Chao Bu, Zhigao Chen, Hualan Ge, Jinying Wen, Zhiyuan Chen, Weiye Shi, Jianzhong Zeng, Xianying
Source	PMC
abstract	We established two Madin-Darby canine kidney (MDCK) cell lines stably expressing human airway transmembrane protease: transmembrane protease, serine 2 (TMPRSS2) and mosaic serine protease large form (MSPL) which support multicycle growth of two H5 highly pathogenic avian influenza viruses (HPAIV) recombinant vaccines (Re-5 and Re-6) and an H9 avian influenza virus (AIV) recombinant vaccine (Re-9) in the absence of trypsin. Data showed that the cell lines stably expressed TMPRSS2 and MSPL after 20 serial passages. Both MDCK-TMPRSS2 and MDCK-MSPL could proteolytically cleave the HA of Re-5, Re-6, and Re-9 and supported high-titer growth of the vaccine without exogenous trypsin. Re-5, Re-6, and Re-9 efficiently infected and replicated within MDCK-TMPRSS2 and MDCK-MSPL cells and viral titer were comparable to the virus grown in MDCK cells with TPCK-trypsin. Thus, our results indicate a potential application for these cell lines in cell-based influenza vaccine production and may serve as a useful tool for HA proteolytic cleavage-related studies.
has issue date	2015-03-30(xsd:dateTime)
bibo:doi	10.1155/2015/402628
bibo:pmid	25918647
has license	cc-by
sha1sum (hex)	3521d912c060861a0bdeeb1607d2e262d62707fa
schema:url	https://doi.org/10.1155/2015/402628
resource representing a document's title	Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin
has PubMed Central identifier	PMC4396729
has PubMed identifier	25918647
schema:publication	Biotechnol Res Int
resource representing a document's body	covid:3521d912c060861a0bdeeb1607d2e262d62707fa#body_text
is schema:about of	named entity 'titer' named entity 'supported' named entity 'studies' named entity 'AIV' named entity 'virus' named entity 'virus' named entity 'serine' named entity 'cell lines' named entity 'mosaic' named entity 'cells' named entity 'Cell' named entity 'Establishment' named entity 'Viruses' named entity 'LARGE' named entity 'TRANSMEMBRANE' named entity 'GROWTH' named entity 'INFLUENZA VACCINE' named entity 'AVIAN INFLUENZA VIRUS' named entity 'VACCINE PRODUCTION' named entity 'RECOMBINANT VACCINE' named entity 'KIDNEY' named entity 'transmembrane' named entity 'human' named entity 'protease' named entity 'proteolytic' named entity 'large' named entity 'comparable' named entity 'viral' named entity 'proteolytically' named entity 'passages' named entity 'kidney' named entity 'canine' named entity 'cleave' named entity 'Trypsin' named entity 'MDCK' named entity 'TMPRSS2' named entity 'proteolytically' named entity 'kidney' named entity 'MDCK' named entity 'TMPRSS2' named entity 'canine' named entity 'cell lines' named entity 'titer' named entity 'serine protease' named entity 'MSPL' named entity 'TMPRSS2' named entity 'Influenza Vaccine' named entity 'monobasic' named entity 'MDCK' named entity 'viruses' named entity 'TMPRSS2' named entity 'amino acids' named entity 'anti-FLAG' named entity 'Spanish flu' named entity 'target gene' named entity 'FBS' named entity 'flow cytometry' named entity 'exogenous' named entity 'Takara' named entity 'TMPRSS2' named entity 'furin' named entity 'IRES' named entity 'infectivity' named entity 'influenza vaccines' named entity 'gene' named entity 'low pH' named entity 'influenza virus' named entity 'zoonotic' named entity 'anti-FLAG' named entity 'San Jose' named entity 'China' named entity 'MDCK' named entity 'TMPRSS2' named entity 'influenza viruses'

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