About: Screening of immune-related differentially expressed genes from primary lymphatic organs of broilers fed with probiotic bacillus cereus PAS38 based on suppression subtractive hybridization

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About: Screening of immune-related differentially expressed genes from primary lymphatic organs of broilers fed with probiotic bacillus cereus PAS38 based on suppression subtractive hybridization Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Screening of immune-related differentially expressed genes from primary lymphatic organs of broilers fed with probiotic bacillus cereus PAS38 based on suppression subtractive hybridization
Creator	Luo, Qihui Zeng, Yan Jing, Bo Khalique, Abdul Li, Jiajun Li, Wanqiang Ni, Xueqin Wang, Zhenhua Wang, Junrui Yang, Miao Zeng, Dong Zhang, Dongmei Pan Id, Kangcheng
source	Medline; PMC
abstract	To explore the molecular mechanism of the effect of Bacillus cereus PAS38 on the immunity of broilers, sixty 7-day-old broilers were divided into two groups with three replicates. The control group was fed with basal diet, and the treatment group was fed with basal diet containing Bacillus cereus PAS38 1×10(6) CFU/g. Thymus and bursa of fabricius were taken from two groups of broilers at the age of 42 days, total RNA was extracted, differential gene library was constructed by SSH technology, and immune-related differential genes were screened. Then, we used siRNA to interfere with the expression of some differential genes in the original generation lymphocytes of broiler blood to detect the change of cytokines mRNA expression level. A total of 42 immune-related differentially expressed genes were screened, including 22 up-regulated genes and 20 down-regulated genes. When 7 differentially up-regulated genes associated with enhanced immune function were interfered with in lymphocytes, some immune-promoting cytokines were down-regulated. These results showed that Bacillus cereus PAS38 might up-regulate the expression of JCHAIN, PRDX1, CD3E, CDK6 and other genes in immune organs of broilers, thereby affecting the development of immune organs, the expression of various cytokines and the transduction of immune signals, improving the immune capacity of broilers.
has issue date	2020-07-01(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0235476
bibo:pmid	32609751
has license	cc-by
sha1sum (hex)	3544fd76e257827ef7510f8570ad116b97119396
schema:url	https://doi.org/10.1371/journal.pone.0235476
resource representing a document's title	Screening of immune-related differentially expressed genes from primary lymphatic organs of broilers fed with probiotic bacillus cereus PAS38 based on suppression subtractive hybridization
has PubMed Central identifier	PMC7329121
has PubMed identifier	32609751
schema:publication	PLoS One
resource representing a document's body	covid:3544fd76e257827ef7510f8570ad116b97119396#body_text
is schema:about of	named entity 'extracted' named entity 'cytokines' named entity 'diet' named entity 'SSH' named entity 'siRNA' named entity 'https' named entity 'development' named entity 'gene' named entity 'probiotic' named entity 'RESULTS' named entity 'SIRNA' named entity 'GROUPS' named entity 'GENES' named entity 'SSH' named entity 'DAYS' named entity 'immunity' named entity 'screened' named entity 'up-regulated' named entity 'generation' named entity 'diet' named entity 'original' named entity 'expression' named entity 'genes' named entity 'broilers' named entity 'control group' named entity 'bursa of fabricius' named entity 'probiotic' named entity 'down-regulated' named entity 'lymphocytes' named entity 'differentially expressed genes' named entity 'Bacillus cereus' named entity 'lymphatic organs' named entity 'RNA' named entity 'subtractive hybridization' named entity 'broilers' named entity 'constructed' named entity 'TLR4' named entity 'IL-2' named entity 'signaling pathways' named entity 'peripheral blood mononuclear cells' named entity 'treatment group' named entity 'BTLA' named entity 'lymphocytes' named entity 'tumor' named entity 'TLR7' named entity 'stomach acid' named entity 'hormones' named entity 'Lactobacillus bulgaricus' named entity 'lymphocytes' named entity 'IFN-γ' named entity 'siRNA' named entity 'IRF1' named entity 'synovium' named entity 'Sichuan Agricultural University' named entity 'IGF1R' named entity 'differentially expressed genes' named entity 'intracellular signal transduction' named entity 'lymphocytes' named entity 'amino acid' named entity 'subtractive hybridization' named entity 'ribosomal' named entity 'CDK6' named entity 'broilers' named entity 'protein' named entity 'antigen recognition' named entity 'ESTs' named entity 'Bacillus cereus' named entity 'broilers' named entity 'RNA'

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