About: Validation of SARS-CoV-2 detection across multiple specimen types

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About: Validation of SARS-CoV-2 detection across multiple specimen types Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Validation of SARS-CoV-2 detection across multiple specimen types
Creator	Jerome, Keith Nalla, Arun Perchetti, Garrett Huang, Meei-Li Greninger, Alexander Wei, Yulun Ak, Nalla Al, Greninger Ga, Perchetti Huang, M-Li Kr, Jerome Loprieno, Michelle Ma, Loprieno Stensland, Larry Zhu, Haiying
source	Elsevier; Medline; PMC
abstract	BACKGROUND: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused considerable disruption across the world, resulting in more than 235,000 deaths since December 2019. SARS-CoV-2 has a wide tropism and detection of the virus has been described in multiple specimen types, including various respiratory secretions, cerebrospinal fluid, and stool. OBJECTIVE: To evaluate the accuracy and sensitivity of a laboratory modified CDCbased SARS-CoV-2 N1 and N2 assay across a range of sample types. Study Design We compared the matrix effect on the analytical sensitivity of SARS-CoV-2 detection by qRT-PCR in nasal swabs collected in viral transport medium (VTM), bronchoalveolar lavage (BAL), sputum, plasma, cerebral spinal fluid (CSF), stool, VTM, phosphate buffered saline (PBS), and Hanks' Balanced Salt Solution (HBSS). Initial limits of detection (LoD) were subsequently narrowed to confirm an LoD for each specimen type and target gene. RESULTS: LoDs were established using a modified CDC-based laboratory developed test and ranged from a mean CT cut-off of 33.8-35.7 (10-20 copies/reaction) for the N1 gene target, and 34.0-36.2 (1-10 copies/reaction) for N2. Alternatives to VTM such as PBS and HBSS had comparable LoDs. The N2 gene target was found to be most sensitive in CSF. CONCLUSION: A modified CDC-based laboratory developed test is able to detect SARSCoV- 2 accurately with similar sensitivity across all sample types tested.
has issue date	2020-05-13(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2020.104438
bibo:pmid	32470887
has license	no-cc
sha1sum (hex)	35b110d2a8debd34f8fed8239fd804086e8196f4
schema:url	https://doi.org/10.1016/j.jcv.2020.104438
resource representing a document's title	Validation of SARS-CoV-2 detection across multiple specimen types
has PubMed Central identifier	PMC7219399
has PubMed identifier	32470887
schema:publication	J Clin Virol
resource representing a document's body	covid:35b110d2a8debd34f8fed8239fd804086e8196f4#body_text
is schema:about of	named entity 'TYPES' covid:arg/35b110d2a8debd34f8fed8239fd804086e8196f4 named entity 'Word' named entity 'SARS-CoV-2' named entity 'PCR' named entity 'COVID' named entity 'viruses' named entity 'LoDs' named entity 'EXO' named entity 'Viral' named entity 'sputum' named entity 'virus' named entity 'plasma' named entity 'PCR' named entity 'HBSS' named entity 'VTM' named entity 'CSF' named entity 'plasma' named entity 'cross-reactivity' named entity 'VTM' named entity 'HeLa cells' named entity 'CDC' named entity 'kidney' named entity 'lung' named entity 'LoDs' named entity 'LoDs' named entity 'BIO-RAD' named entity 'sputum' named entity 'CDC' named entity 'EXO' named entity 'qRT-PCR' named entity 'cross-reactivity' named entity 'CSF' named entity 'qRT-PCR' named entity 'COVID-19 testing' named entity 'COVID' named entity 'HBSS' named entity 'aspirates' named entity 'SARS-CoV-2' named entity 'PBS' named entity 'gene' named entity 'phosphate buffered saline' named entity 'plasma' named entity 'nmol/L' named entity 'LoDs' named entity 'SARS-CoV-2' named entity 'Hanks' Balanced Salt Solution' named entity 'Severe Acute Respiratory Syndrome Coronavirus 2' named entity 'qRT-PCR' named entity 'virus' named entity 'virus' named entity 'RNA' named entity 'viral pathogens' named entity 'cross-reactivity' named entity 'VTM' named entity 'VTM' named entity 'bronchoalveolar lavage' named entity 'virus' named entity 'SARS-CoV-2' named entity 'plasma' named entity 'RNA' named entity 'RNA' named entity 'invasive procedure' named entity 'LoDs' named entity 'SARS-CoV-2' named entity 'pathogen' named entity 'RNA transcript'

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