About: An evaluation of porcine epidemic diarrhea virus survival in individual feed ingredients in the presence or absence of a liquid antimicrobial

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About: An evaluation of porcine epidemic diarrhea virus survival in individual feed ingredients in the presence or absence of a liquid antimicrobial Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An evaluation of porcine epidemic diarrhea virus survival in individual feed ingredients in the presence or absence of a liquid antimicrobial
Creator	Christopher-Hennings, Jane Nelson, Eric Clement, Travis Dee, Scott Singrey, Aaron Neill, Casey
Source	Medline; PMC
abstract	BACKGROUND: Contaminated complete feed and porcine plasma are risk factors for PEDV introduction to farms and a liquid antimicrobial has been proven useful for reducing risk. This study provides information on the survivability of PEDV across common swine feed ingredients in the presence or absence of the liquid antimicrobial. RESULTS: Eighteen ingredients commonly included in commercial swine diets were selected, including 3 grain sources (corn, soybean meal (SBM), dried distillers grains with solubles (DDGS)), 5 porcine by-products (spray-dried plasma, purified plasma, intestinal mucosa, meat and bone meal and red blood cells (RBCs)), 3 vitamin/trace mineral (VTM) mixes (sow, nursery, finishing), 2 fat sources (choice white grease and soy oil), 3 synthetic amino acids (lysine HCL, D/L methionine, threonine), as well as limestone and dry choline chloride. Complete feed and stock PEDV served as controls. Thirty grams of each ingredient were inoculated with 2 mL PEDV. A matched set of samples were treated with the formaldehyde-based liquid antimicrobial SalCURB® (LA). All samples (n = 320) were stored outdoors under winter time ambient conditions for 30 days. Samples were submitted on 1, 7, 14 and 30 days post-inoculation (DPI) and tested by PCR and virus isolation (VI). All VI-negative samples were tested by swine bioassay. Viable PEDV was detected by VI or swine bioassay at 1, 7, 14 and 30 DPI from SBM, DDGS, meat & bone meal, RBCs, lysine HCL, D/L methionine, choice white grease, choline chloride, complete feed and stock virus control and at 7 DPI in limestone and at 14 DPI in threonine. Supplementary testing of complete feed and SBM indicated viable virus out to 45 and 180 DPI, respectively. All other samples were negative by VI and bioassay. In contrast, treatment with LA inactivated PEDV across all ingredients on 1 DPI and induced RNA reduction over time. CONCLUSIONS: Under the conditions of this study, PEDV viability in feed was influenced by ingredient with extended survival in SBM. Furthermore, LA treatment rendered virus inactive, independent of ingredient type.
has issue date	2015-07-09(xsd:dateTime)
bibo:doi	10.1186/s40813-015-0003-0
bibo:pmid	28405416
has license	cc-by
sha1sum (hex)	35b51eaf2cbbb07dd83420b7185215a2f9467709
schema:url	https://doi.org/10.1186/s40813-015-0003-0
resource representing a document's title	An evaluation of porcine epidemic diarrhea virus survival in individual feed ingredients in the presence or absence of a liquid antimicrobial
has PubMed Central identifier	PMC5382523
has PubMed identifier	28405416
schema:publication	Porcine Health Manag
resource representing a document's body	covid:35b51eaf2cbbb07dd83420b7185215a2f9467709#body_text
is schema:about of	named entity 'Thirty' named entity 'Samples' named entity 'contrast' named entity 'D/L' named entity 'samples' named entity 'study' named entity 'white' named entity 'matched' named entity 'DDGS' named entity 'liquid' named entity 'feed' named entity 'corn' named entity 'Background' named entity 'induced' named entity 'grease' named entity 'This' named entity 'conditions' named entity 'detected' named entity 'liquid' named entity 'fat' named entity 'bioassay' named entity 'plasma' named entity 'threonine' named entity 'lysine' named entity 'liquid' named entity 'feed' named entity 'purified' named entity 'plasma' named entity 'porcine' named entity 'virus' named entity 'distillers grains' named entity 'trace mineral' named entity 'D/L' named entity 'threonine' named entity 'HCL' named entity 'antimicrobial' named entity 'plasma' named entity 'risk factors' named entity 'meat and bone meal' named entity 'RBCs' named entity 'vitamin' named entity 'by-products' named entity 'formaldehyde' named entity 'amino acids' named entity 'serially diluted' named entity 'negative control' named entity 'pathogen' named entity 'meat and bone meal' named entity 'intravenous' named entity 'Dacron' named entity 'PEDV' named entity 'threonine' named entity 'by-products' named entity 'sodium pentobarbital' named entity 'SDSU' named entity 'risk factors' named entity 'trypsin' named entity 'PEDV' named entity 'limestone' named entity 'plastic' named entity 'protein' named entity 'follow-up' named entity 'by-products' named entity 'upper Midwest' named entity 'plasma' named entity 'Weather Underground' named entity 'distillers grains' named entity 'PEDV' named entity 'antimicrobial' named entity '5.5' named entity 'bioassay' named entity 'bioassay' named entity 'Rockville, MD' named entity 'TPCK' named entity 'lysine' named entity 'syringe'

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