About: Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco

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About: Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco
Creator	Khataby, Khadija El Houadfi, Mohammed Fellahi, Siham Sebbar, Ghizlane Bouaiti, El Ennaji, My Fihri, Ouafae Harrak, Mehdi Kuhn, Jens
Source	Medline; PMC
abstract	BACKGROUND: A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries. METHODS: In this study, an easy-to-perform SYBR green I real-time reverse transcriptase polymerase chain reaction (RT-PCR) targeting the nucleocapsid gene of IBV was developed and compared with conventional agarose gel-based RT-PCR for the detection of IBV infection. RESULTS: We found that the SYBR green I real-time RT-PCR was at least 10 times more sensitive than the agarose gel electrophoresis detection method. The assay exhibited high specificity for IBV infection. All negative controls, such as Newcastle disease virus, infectious bursal disease virus, and avian influenza virus, were not detected. CONCLUSION: The SYBR green I real-time RT-PCR test described herein can be used to rapidly distinguish IBV from other respiratory pathogens, which is important for diagnosis and control of infectious bronchitis outbreaks in Morocco. The test is a valuable and useful method as a routine assay for diagnosis of clinical IBV infection in commercial chickens.
has issue date	2016-04-22(xsd:dateTime)
bibo:doi	10.1186/s13104-016-2037-z
bibo:pmid	27106608
has license	cc-by
sha1sum (hex)	3619c9e99ff6ceb2fa038d13239e6fce4e818995
schema:url	https://doi.org/10.1186/s13104-016-2037-z
resource representing a document's title	Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco
has PubMed Central identifier	PMC4841946
has PubMed identifier	27106608
schema:publication	BMC Res Notes
resource representing a document's body	covid:3619c9e99ff6ceb2fa038d13239e6fce4e818995#body_text
is schema:about of	named entity 'SYBR green I' named entity 'infection' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'BACKGROUND' named entity 'SPECIFIC' named entity 'COUNTRIES' named entity 'OUTBREAKS' named entity 'AGAROSE GEL' named entity 'CONVENTIONAL' named entity 'VIRUS INFECTION' named entity 'BASED' named entity 'RAPID' named entity 'REAL-TIME RT-PCR' named entity 'MOROCCO' named entity 'INFECTION' named entity 'MOLECULAR METHOD' named entity 'SYBR GREEN I' named entity 'CHICKENS' named entity 'RT-PCR' named entity 'COMPARISON' named entity 'IMPORTANT' named entity 'SENSITIVE' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'DIAGNOSIS' named entity 'MOROCCO' named entity 'DIAGNOSIS' named entity 'INFECTIOUS BRONCHITIS' named entity 'CURBING' covid:arg/3619c9e99ff6ceb2fa038d13239e6fce4e818995 named entity 'sensitive, and specific' named entity 'agarose gel' named entity 'positive-sense, single-stranded RNA' named entity 'genetic material' named entity 'coronaviruses' named entity 'nasal discharge' named entity 'RT-PCR' named entity 'SYBR green' named entity 'diethyl pyrocarbonate' named entity 'RT PCR' named entity 'SYBR green' named entity 'protein' named entity 'Infectious bronchitis' named entity 'real-time RT-PCR' named entity 'genome' named entity 'RT-PCR' named entity 'Rabat' named entity 'fluorescence' named entity 'Promega' named entity 'SYBR green' named entity 'genus' named entity 'PCR' named entity 'poultry industry' named entity 'simple linear regression' named entity 'RNA' named entity 'infection' named entity 'avian influenza' named entity 'PCR reaction' named entity 'vaccine strain' named entity 'IBV' named entity 'RT-PCR' named entity 'Sunnyvale' named entity 'viral RNA' named entity 'fluorescent probe' named entity 'cDNA' named entity 'Thermal Cycler' named entity 'primer-dimer' named entity 'Moroccan' named entity 'cDNA' named entity 'serially diluted' named entity 'rales' named entity 'IBDV' named entity 'IBV' named entity 'Moroccan'

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