About: Posttranslational Control of PlsB Is Sufficient To Coordinate Membrane Synthesis with Growth in Escherichia coli

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Posttranslational Control of PlsB Is Sufficient To Coordinate Membrane Synthesis with Growth in Escherichia coli Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Posttranslational Control of PlsB Is Sufficient To Coordinate Membrane Synthesis with Growth in Escherichia coli
Creator	Bokinsky, Gregory Büke, Ferhat Imholz, Nicole Noga, Marek Scherer, Nicole Van Den Broek, Niels Yang, Flora
Source	PMC
abstract	Every cell must produce enough membrane to contain itself. However, the mechanisms by which the rate of membrane synthesis is coupled with the rate of cell growth remain unresolved. By comparing substrate and enzyme concentrations of the fatty acid and phospholipid synthesis pathways of Escherichia coli across a 3-fold range of carbon-limited growth rates, we show that the rate of membrane phospholipid synthesis during steady-state growth is determined principally through allosteric control of a single enzyme, PlsB. Due to feedback regulation of the fatty acid pathway, PlsB activity also indirectly controls synthesis of lipopolysaccharide, a major component of the outer membrane synthesized from a fatty acid synthesis intermediate. Surprisingly, concentrations of the enzyme that catalyzes the committed step of lipopolysaccharide synthesis (LpxC) do not differ across steady-state growth conditions, suggesting that steady-state lipopolysaccharide synthesis is modulated primarily via indirect control by PlsB. In contrast to steady-state regulation, we found that responses to environmental perturbations are triggered directly via changes in acetyl coenzyme A (acetyl-CoA) concentrations, which enable rapid adaptation. Adaptations are further modulated by ppGpp, which regulates PlsB activity during slow growth and growth arrest. The strong reliance of the membrane synthesis pathway upon posttranslational regulation ensures both the reliability and the responsiveness of membrane synthesis.
has issue date	2020-08-18(xsd:dateTime)
bibo:doi	10.1128/mbio.02703-19
bibo:pmid	32817111
has license	cc-by
sha1sum (hex)	368b8db0b35cfb97f4b375a4cf573d38019376cc
schema:url	https://doi.org/10.1128/mbio.02703-19
resource representing a document's title	Posttranslational Control of PlsB Is Sufficient To Coordinate Membrane Synthesis with Growth in Escherichia coli
has PubMed Central identifier	PMC7439487
has PubMed identifier	32817111
schema:publication	mBio
resource representing a document's body	covid:368b8db0b35cfb97f4b375a4cf573d38019376cc#body_text
is schema:about of	named entity 'modulated' named entity 'individual' named entity 'phospholipid' named entity 'control' named entity 'ensure' named entity 'enzyme' named entity 'Although' named entity 'biogenesis' named entity 'concentrations' named entity 'Coordinate' named entity 'INDIRECT' named entity 'ENSURES' named entity 'CELL' named entity 'RELIANCE' named entity 'OUTER MEMBRANE' named entity 'SPECIES' named entity 'DEACTIVATE' named entity 'RATE' named entity 'BIOCHEMISTRY' named entity 'DIRECTLY' named entity 'RESOLVE' named entity 'GROW' named entity 'ACROSS' named entity 'ESCHERICHIA COLI' named entity 'COMPONENT' named entity 'ENZYME' named entity 'SYNTHESIS' named entity 'A FATTY ACID' named entity 'ENVIRONMENTAL' named entity 'PHOSPHOLIPID SYNTHESIS' named entity 'METABOLISM' named entity 'CONTROL' named entity 'MODULATED' named entity 'TRIGGERED' named entity 'ENOUGH' named entity 'CONDITIONS' named entity 'MEMBRANES' named entity 'BALANCE' named entity 'acetyl-CoA' named entity 'contrast' named entity 'synchronized' named entity 'outer membrane' named entity 'indirectly' named entity 'membrane' named entity 'Escherichia coli' named entity 'breaking' named entity 'steady-state' named entity 'species' named entity 'bacterial cells' named entity 'comparing' named entity 'activate' named entity 'enzyme' named entity 'fatty acid' named entity 'fatty acid' named entity 'membrane' named entity 'membrane' named entity 'coupled' named entity 'activity' named entity 'remain' named entity 'concentrations' named entity 'biochemistry' named entity 'committed step' named entity 'metabolism' named entity 'steady-state' named entity 'outer membrane' named entity 'enzyme' named entity 'catalyzes' named entity 'enzyme' named entity 'fatty acid' named entity 'ppGpp' named entity 'citric acid' named entity '0.80' named entity 'branch point' named entity '37°C' named entity 'formic acid'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software