About: Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

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About: Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time
Creator	Barnes, Kayla
Source	Medline; PMC
abstract	Recent outbreaks of viral hemorrhagic fevers (VHFs), including Ebola virus disease (EVD) and Lassa fever (LF), highlight the urgent need for sensitive, deployable tests to diagnose these devastating human diseases. Here we develop CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting Ebola virus (EBOV) and Lassa virus (LASV), with both fluorescent and lateral flow readouts. We demonstrate on laboratory and clinical samples the sensitivity of these assays and the capacity of the SHERLOCK platform to handle virus-specific diagnostic challenges. We perform safety testing to demonstrate the efficacy of our HUDSON protocol in heat-inactivating VHF viruses before SHERLOCK testing, eliminating the need for an extraction. We develop a user-friendly protocol and mobile application (HandLens) to report results, facilitating SHERLOCK’s use in endemic regions. Finally, we successfully deploy our tests in Sierra Leone and Nigeria in response to recent outbreaks.
has issue date	2020-08-17(xsd:dateTime)
bibo:doi	10.1038/s41467-020-17994-9
bibo:pmid	32807807
has license	cc-by
sha1sum (hex)	36a871e4a53df682779c678f537b12362f954960
schema:url	https://doi.org/10.1038/s41467-020-17994-9
resource representing a document's title	Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time
has PubMed Central identifier	PMC7431545
has PubMed identifier	32807807
schema:publication	Nat Commun
resource representing a document's body	covid:36a871e4a53df682779c678f537b12362f954960#body_text
is schema:about of	named entity 'fluorescent' named entity 'deploy' named entity 'Ebola virus' named entity 'efficacy' named entity 'highlight' named entity 'protocol' named entity 'user-friendly' named entity 'Nigeria' named entity 'Lassa virus' named entity 'SAMPLES' named entity 'DEPLOY' named entity 'lateral' named entity 'SHERLOCK' named entity 'extraction' named entity 'diseases' named entity 'facilitating' named entity 'regions' named entity 'develop' named entity 'testing' named entity 'Sierra Leone' named entity 'virus' named entity 'CRISPR' named entity 'EBOV' named entity 'LASV' named entity 'adaptive histogram equalization' named entity 'viruses' named entity 'blood draw' named entity 'EBOV' named entity 'RT-qPCR' named entity 'EBOV' named entity 'clade' named entity 'trizol' named entity '95% confidence interval' named entity 'userfriendly' named entity 'sliding window' named entity 'dengue virus' named entity 'LASV' named entity 'genomes' named entity 'gene' named entity 'EBOV' named entity 'EVD' named entity 'cross-reactivity' named entity 'EBOV' named entity 'point-of-care test' named entity 'LASV' named entity 'nucleases' named entity 'Sierra Leone' named entity 'mobile device' named entity 'crRNA' named entity 'clades' named entity 'saliva' named entity 'diagnostic tests' named entity 'RT-qPCR' named entity 'cDNA' named entity 'RDT' named entity 'EBOV' named entity 'virus' named entity 'RNA' named entity 'viral species' named entity 'LASV' named entity 'cDNA' named entity 'RNA' named entity 'clade' named entity 'genomes' named entity 'VHF' named entity 'virus' named entity 'genomes' named entity 'signal processing' named entity 'crRNA' named entity 'LASV' named entity 'supportive care' named entity 'CRISPR' named entity 'LASV' named entity 'fluorescence' named entity 'Nigeria' named entity 'Sierra Leone' named entity 'RPA' named entity 'replicant' named entity 'nucleic acid' named entity 'clade' named entity 'synthetic DNA'

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