About: Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro

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About: Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro
Creator	Drosten, Christian Günther, Stephan Asper, Marcel Becker-Ziaja, Beate Borowski, Peter Chen, Huan-Ming Röser, Christina Hosmane, Ramachandra Luna, Luciano
topic	covid:36dccf250d49da67c84d4c18c717c239d6fd7f73#this
Source	Elsevier; Medline; PMC
abstract	Abstract This report describes the application of real-time PCR for testing antivirals against highly pathogenic viruses such as Lassa virus, SARS coronavirus and Ebola virus. The test combines classical cell culture with a quantitative real-time PCR read-out. The assay for Lassa virus was validated with ribavirin, which showed an IC50 of 9μg/ml. Small-scale screening identified a class of imidazole nucleoside/nucleotide analogues with antiviral activity against Lassa virus. The analogues contained either dinitrile or diester groups at the imidazole 4,5-positions, and many of which possessed an acyclic sugar or sugar phosphonate moiety at the imidazole 1-position. The IC50 values of the most active compounds ranged from 5 to 21μg/ml. The compounds also inhibited replication of SARS coronavirus and Ebola virus in analogous assays, although to a lesser extent than Lassa virus.
has issue date	2004-09-30(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2004.05.001
bibo:pmid	15451189
has license	els-covid
sha1sum (hex)	36dccf250d49da67c84d4c18c717c239d6fd7f73
schema:url	https://doi.org/10.1016/j.antiviral.2004.05.001
resource representing a document's title	Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro
has PubMed Central identifier	PMC7126008
has PubMed identifier	15451189
schema:publication	Antiviral Research
resource representing a document's body	covid:36dccf250d49da67c84d4c18c717c239d6fd7f73#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/36dccf250d49da67c84d4c18c717c239d6fd7f73
is schema:about of	named entity 'screening' named entity 'assay' named entity 'SARS coronavirus' named entity 'REAL-TIME PCR ' named entity 'SMALL' named entity 'REPLICATION' named entity 'VIRUSES' named entity 'REAL-TIME PCR ' named entity 'LASSA VIRUS' named entity 'IMIDAZOLE' named entity 'VALIDATED' named entity 'CELL CULTURE' named entity 'LESSER' named entity '2C5' named entity 'SARS CORONAVIRUS' named entity 'IDENTIFIED' named entity 'ANTIVIRALS' named entity 'GROUPS' named entity 'POSITION' named entity 'RIBAVIRIN' named entity 'COMBINES' named entity 'HIGHLY' named entity 'DIESTER' named entity 'ANTIVIRAL' named entity 'POSSESSED' named entity 'PATHOGENIC' named entity 'TESTING' named entity 'READ' named entity 'SARS CORONAVIRUS' named entity 'LASSA VIRUS' named entity 'EBOLA VIRUS' named entity 'APPLICATION' named entity 'NUCLEOSIDE' named entity 'QUANTITATIVE REAL-TIME PCR' named entity 'SCREENING' named entity 'CLASSICAL' named entity 'REPORT' named entity 'A CLASS' named entity 'SUGAR' named entity 'MOIETY' named entity 'EITHER' named entity 'DINITRILE' named entity 'EBOLA VIRUS' named entity 'EXTENT' named entity 'POSITIONS' named entity 'ANTIVIRAL ACTIVITY' named entity 'APPLICATION' named entity 'PHOSPHONATE' named entity 'VALUES' named entity 'ASSAY' named entity 'ACYCLIC' named entity 'TEST' named entity 'IN VITRO' named entity 'INHIBITED' named entity 'CONTAINED' named entity 'ACTIVE' named entity 'SCALE' named entity 'TESTING' covid:arg/36dccf250d49da67c84d4c18c717c239d6fd7f73 named entity 'application' named entity 'classical' named entity 'position' named entity 'assays' named entity 'groups' named entity 'real-time PCR' named entity 'identified' named entity 'Lassa virus' named entity 'sugar' named entity 'imidazole' named entity 'ribavirin' named entity 'pathogenic viruses'

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