About: Performance evaluation of ExiStation HBV diagnostic system for hepatitis B virus DNA quantitation

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About: Performance evaluation of ExiStation HBV diagnostic system for hepatitis B virus DNA quantitation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Performance evaluation of ExiStation HBV diagnostic system for hepatitis B virus DNA quantitation
Creator	Sohn, Yong-Hak Kim, Hyun Cha, Young Yoo, Soo
source	Elsevier; Medline; PMC
abstract	The performance of a recently developed real-time PCR system, the ExiStation HBV diagnostic system, for quantitation of hepatitis B virus (HBV) in human blood was evaluated. The detection limit, reproducibility, cross-reactivity, and interference were evaluated as measures of analytical performance. For the comparison study, 100 HBV-positive blood samples and 100 HBV-negative samples from Korean Blood Bank Serum were used, and the results of the ExiStation HBV system showed good correlation with those obtained using the Cobas TaqMan (r(2) = 0.9931) and Abbott real-time PCR systems (r(2) = 0.9894). The lower limit of detection was measured as 9.55 IU/mL using WHO standards and the dynamic range was linear from 6.68 to 6.68 × 10(9) IU/mL using cloned plasmids. The within-run coefficient of variation (CV) was 9.4%, 2.1%, and 1.1%, and the total CV was 11.8%, 3.6%, and 1.7% at a concentration of 1.92 log(10) IU/mL, 3.88 log(10) IU/mL, and 6.84 log(10) IU/mL, respectively. No cross-reactivity or interference was detected. The ExiStation HBV diagnostic system showed satisfactory analytical sensitivity, excellent reproducibility, no cross-reactivity, no interference, and high agreement with the Cobas TaqMan and Abbott real-time PCR systems, and is therefore a useful tool for the detection and monitoring of HBV infection.
has issue date	2013-07-25(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2013.07.027
bibo:pmid	23892129
has license	no-cc
sha1sum (hex)	37e67b3fa1f8e9b422b009e613043f6aebdb0b20
schema:url	https://doi.org/10.1016/j.jviromet.2013.07.027
resource representing a document's title	Performance evaluation of ExiStation HBV diagnostic system for hepatitis B virus DNA quantitation
has PubMed Central identifier	PMC7119692
has PubMed identifier	23892129
schema:publication	J Virol Methods
resource representing a document's body	covid:37e67b3fa1f8e9b422b009e613043f6aebdb0b20#body_text
is schema:about of	named entity 'developed' named entity 'good' named entity 'The' named entity 'systems' named entity 'EVALUATION' named entity 'QUANTITATION' named entity 'LOG' named entity 'HBV' named entity 'CORRELATION' named entity 'GOOD' named entity 'REAL-TIME PCR ' named entity 'SATISFACTORY' named entity 'USEFUL' named entity 'MONITORING' named entity '3.6' named entity '100' named entity '2.1' named entity 'EXCELLENT' named entity 'COMPARISON' named entity 'INTERFERENCE' named entity 'PERFORMANCE' named entity 'BLOOD BANK' named entity 'CONCENTRATION OF' named entity 'DETECTION' named entity 'ANALYTICAL' named entity 'TOOL' covid:arg/37e67b3fa1f8e9b422b009e613043f6aebdb0b20 named entity 'HBV' named entity 'DETECTION LIMIT' named entity 'EVALUATED' named entity 'LINEAR' named entity 'RECENTLY' named entity '9.4' named entity 'PLASMIDS' named entity 'USED' named entity 'MEASURES' named entity 'BLOOD SAMPLES' named entity 'NEGATIVE' named entity 'OBTAINED' named entity 'HBV INFECTION' named entity 'HIGH' named entity 'DIAGNOSTIC SYSTEM' named entity 'REPRODUCIBILITY' named entity 'ABBOTT' named entity 'HEPATITIS B VIRUS' named entity 'HUMAN BLOOD' named entity 'CROSS' named entity 'RUN' named entity '1.7' named entity 'COEFFICIENT OF VARIATION' named entity 'SYSTEMS' named entity 'QUANTITATION' named entity 'PERFORMANCE' named entity 'DIAGNOSTIC SYSTEM' named entity 'SENSITIVITY' named entity 'SERUM' named entity 'TAQMAN' named entity 'SYSTEM' named entity 'SAMPLES' named entity 'KOREAN' named entity 'CLONED' named entity 'TOTAL' named entity 'RESULTS' named entity 'STANDARDS' named entity 'HEPATITIS B VIRUS DNA' named entity 'AGREEMENT' named entity 'REACTIVITY' named entity 'POSITIVE' named entity 'MEASURED' named entity 'USING' named entity 'STUDY' named entity 'DYNAMIC RANGE' named entity '1.1' named entity 'DETECTED' named entity 'detected' named entity 'HBV' named entity 'study' named entity 'interference'

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