About: Background: Inflammation of the central nervous system (CNS) is a frequent condition in cats but etiology often remains unsolved. Routine cerebrospinal fluid (CSF) analysis can be extended through the calculation of the albumin quotient (Q (alb)), a marker of the integrity of the blood–brain barrier (BBB), and IgG index, an estimate of intrathecal IgG synthesis. Objectives: The purpose of this study was to validate nephelometric methods for CSF protein analysis, and to use the Q (alb) and IgG index to discriminate blood‐ and brain‐derived immunoglobulin fractions in cats with feline infectious peritonitis (FIP). Methods: Cats presented to our clinic between 2001 and 2005 were included in the study based on clinical and laboratory data and histopathologic findings at necropsy. Cats were grouped as having nonneurologic disease (controls; n=37), brain tumors (n=8), FIP involving the CNS (n=12), and extraneural FIP (n=12). CSF‐total protein (TP) was measured and albumin and IgG concentrations were measured in paired CSF/serum samples; Q (alb) and IgG index were calculated. Intraassay and interassay precision of the nephelometric assays were determined using pooled samples. Results: Coefficients of variation for the nephelometric assays ranged from 2.7% to 7.2%. In control cats, CSF‐TP concentration ranged from 0.06 to 0.36 g/L, Q (alb) ranged from 0.6 to 5.7 × 10(−3), and IgG index ranged from 0.3 to 0.6. Q (alb) and IgG index were significantly higher in cats with brain tumors and cats with CNS‐FIP compared with other groups. Compared with control cats, pleocytosis was evident in 8 of 12 (67%) cats and CSF‐TP was increased in 3 of 12 (25%) cats with CNS‐FIP. Conclusion: Nephelometry is a reliable method for measurement of CSF protein, albumin, and IgG in cats. The Q (alb) and IgG index did not identify a CSF protein pattern specific for BBB dysfunction or intrathecal IgG synthesis in cats with CNS‐FIP.   Goto Sponge  NotDistinct  Permalink

An Entity of Type : fabio:Abstract, within Data Space : wasabi.inria.fr associated with source document(s)

AttributesValues
type
value
  • Background: Inflammation of the central nervous system (CNS) is a frequent condition in cats but etiology often remains unsolved. Routine cerebrospinal fluid (CSF) analysis can be extended through the calculation of the albumin quotient (Q (alb)), a marker of the integrity of the blood–brain barrier (BBB), and IgG index, an estimate of intrathecal IgG synthesis. Objectives: The purpose of this study was to validate nephelometric methods for CSF protein analysis, and to use the Q (alb) and IgG index to discriminate blood‐ and brain‐derived immunoglobulin fractions in cats with feline infectious peritonitis (FIP). Methods: Cats presented to our clinic between 2001 and 2005 were included in the study based on clinical and laboratory data and histopathologic findings at necropsy. Cats were grouped as having nonneurologic disease (controls; n=37), brain tumors (n=8), FIP involving the CNS (n=12), and extraneural FIP (n=12). CSF‐total protein (TP) was measured and albumin and IgG concentrations were measured in paired CSF/serum samples; Q (alb) and IgG index were calculated. Intraassay and interassay precision of the nephelometric assays were determined using pooled samples. Results: Coefficients of variation for the nephelometric assays ranged from 2.7% to 7.2%. In control cats, CSF‐TP concentration ranged from 0.06 to 0.36 g/L, Q (alb) ranged from 0.6 to 5.7 × 10(−3), and IgG index ranged from 0.3 to 0.6. Q (alb) and IgG index were significantly higher in cats with brain tumors and cats with CNS‐FIP compared with other groups. Compared with control cats, pleocytosis was evident in 8 of 12 (67%) cats and CSF‐TP was increased in 3 of 12 (25%) cats with CNS‐FIP. Conclusion: Nephelometry is a reliable method for measurement of CSF protein, albumin, and IgG in cats. The Q (alb) and IgG index did not identify a CSF protein pattern specific for BBB dysfunction or intrathecal IgG synthesis in cats with CNS‐FIP.
Subject
  • Neurology
  • Central nervous system
  • Mythology
  • Seed storage proteins
  • Meteorological instrumentation and equipment
part of
is abstract of
is hasSource of
Faceted Search & Find service v1.13.91 as of Mar 24 2020


Alternative Linked Data Documents: Sponger | ODE     Content Formats:       RDF       ODATA       Microdata      About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data]
OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software