About: A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus

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About: A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus
Creator	Yuen, Kwok-Yung Chan, Kwok-Hung Xia, Ningshao Chen, Honglin Chen, Yixin Kang, Yahong Chan, Jasper Lau, Susanna Luk, Hayes Poon, Rosana Woo, Patrick
Source	Medline; PMC
abstract	Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in Escherichia coli. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID(50))/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID(50)/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies.
has issue date	2015-04-22(xsd:dateTime)
bibo:doi	10.1038/emi.2015.26
bibo:pmid	26421268
has license	cc-by-nc-sa
sha1sum (hex)	381d5feb501bd762182e76a3fcb4dd3f64c9a261
schema:url	https://doi.org/10.1038/emi.2015.26
resource representing a document's title	A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus
has PubMed Central identifier	PMC4575394
has PubMed identifier	26421268
schema:publication	Emerg Microbes Infect
resource representing a document's body	covid:381d5feb501bd762182e76a3fcb4dd3f64c9a261#body_text
is schema:about of	named entity 'SPECIFIC' named entity 'LIVE' named entity 'ANTIGEN DETECTION' named entity 'DETECTION' named entity 'MERS CORONAVIRUS' named entity 'CUTOFF' named entity 'STUDY' named entity '0.19' named entity '50%' named entity 'LENGTH' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'SPECIFICITY' named entity 'MORE THAN' named entity 'ELISA' named entity 'ANTIGEN' named entity 'VERY LOW' named entity 'HAVE' named entity 'SERIAL' named entity 'SIMULATED' named entity 'RATE' named entity 'FIELD' named entity 'ADDED' named entity 'DROMEDARIES' named entity 'PROTEIN ' named entity 'RESPIRATORY VIRUSES' named entity 'GENE' named entity 'CONFIRMED' named entity 'NPAs' named entity '2012' named entity 'respiratory' named entity 'Middle East respiratory syndrome' named entity 'sensitive' named entity 'assay' named entity 'SENSITIVE' named entity 'VARIOUS' named entity 'SAMPLES' named entity 'ANTIGEN DETECTION' named entity 'LABORATORY' named entity 'ADDITIONAL' named entity 'AVAILABLE' named entity 'ASPIRATES' named entity '900' named entity 'SENSITIVE' named entity 'INFECTIVE DOSE' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'ASSAY' named entity 'ITS' named entity 'COMMERCIAL' named entity 'NASOPHARYNGEAL' named entity 'DILUTIONS' named entity 'USING' named entity 'USE' named entity 'MERS-COV' named entity 'TCID' named entity 'USEFUL' named entity 'EXPRESSED' named entity 'ASSAY' named entity '30%' named entity 'CAPTURE' named entity 'O10' named entity '200' named entity 'POSITIVE' named entity 'MERS' named entity 'MONOCLONAL ANTIBODIES' named entity 'ADDING' named entity 'TISSUE CULTURE' named entity 'MIDDLE EAST RESPIRATORY SYNDROME ' named entity 'CLONED' named entity 'SPECIFIC ANTIGEN' named entity 'HIGHLY SENSITIVE' named entity 'RESULTS'

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