About: SARS Coronavirus Spike Protein-Induced Innate Immune Response occurs via Activation of the NF-κB pathway in Human Monocyte Macrophages in vitro

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About: SARS Coronavirus Spike Protein-Induced Innate Immune Response occurs via Activation of the NF-κB pathway in Human Monocyte Macrophages in vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS Coronavirus Spike Protein-Induced Innate Immune Response occurs via Activation of the NF-κB pathway in Human Monocyte Macrophages in vitro
Creator	Collins, Arlene Dosch, Susan Mahajan, Supriya
topic	covid:383de94872ec5bbd71ffcc6d87494de12b2a049f#this
Source	Elsevier; Medline; PMC
abstract	A purified recombinant spike (S) protein was studied for its effect on stimulating human peripheral blood monocyte macrophages (PBMC). We examined inflammatory gene mRNA abundances found in S protein-treated PBMC using gene arrays. We identified differential mRNA abundances of genes with functional properties associated with antiviral (CXCL10) and inflammatory (IL-6, IL-8) responses. We confirmed cytokine mRNA increases by real time quantitative(q) RT-PCR or ELISA. We further analyzed the sensitivity and specificity of the prominent IL-8 response. By real time qRT-PCR, S protein was shown to stimulate IL-8 mRNA accumulation in a dose dependent manner while treatment with E protein did not. Also, titration of S protein-specific production and secretion of IL-8 by ELISA showed that the dose of 5.6nM of S produced a significant increase in IL-8 (p=0.003) compared to mock-treated controls. The increase in IL-8 stimulated by a concentration of 5.6 nM of S was comparable to concentrations seen for S protein binding to ACE2 or to neutralizing monoclonal antibody suggesting a physiological relevance. An NF-κB inhibitor, TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone) could suppress IL-8 production and secretion in response to S protein in PBMC and THP-1 cells and in HCoV-229E virus-infected PBMC. Activation and translocation of NF-κB was shown to occur rapidly following exposure of PBMC or THP-1 cells to S protein using a highly sensitive assay for active nuclear NF-κB p65 transcription factor. The results further suggested that released or secreted S protein could activate blood monocytes thru recognition by toll-like receptor (TLR)2 ligand.
has issue date	2009-06-01(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2009.01.005
bibo:pmid	19185596
has license	bronze-oa
sha1sum (hex)	383de94872ec5bbd71ffcc6d87494de12b2a049f
schema:url	https://doi.org/10.1016/j.virusres.2009.01.005
resource representing a document's title	SARS Coronavirus Spike Protein-Induced Innate Immune Response occurs via Activation of the NF-κB pathway in Human Monocyte Macrophages in vitro
has PubMed Central identifier	PMC2699111
has PubMed identifier	19185596
schema:publication	Virus Research
resource representing a document's body	covid:383de94872ec5bbd71ffcc6d87494de12b2a049f#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/383de94872ec5bbd71ffcc6d87494de12b2a049f
is schema:about of	named entity 'production' named entity 'PBMC' named entity 'responses' named entity 'Innate' named entity 'SARS Coronavirus' named entity 'Monocyte' named entity 'NF-κB' named entity 'ASSOCIATED WITH' named entity 'PBMC' named entity 'EXAMINED' named entity 'RESULTS' named entity 'EFFECT' named entity 'NUCLEAR' named entity 'STIMULATE' named entity 'DIFFERENTIAL' named entity 'INFLAMMATORY' named entity 'PROTEIN BINDING' named entity 'PHYSIOLOGICAL' named entity 'ACE2' named entity 'RELEVANCE' named entity 'STIMULATING' named entity 'BLOOD MONOCYTES' named entity 'TOLL-LIKE RECEPTOR' named entity 'EXPOSURE' named entity 'HIGHLY SENSITIVE' named entity 'PROPERTIES' named entity 'OCCUR' named entity 'RESPONSE' named entity 'CHLOROMETHYL' named entity 'HUMAN' named entity 'CYTOKINE ' named entity 'PROTEIN ' named entity 'TREATED' named entity 'VIRUS' named entity 'RESPONSE TO' named entity 'QUANTITATIVE' named entity 'PATHWAY' named entity 'L-PHENYLALANINE ' named entity 'KETONE' named entity 'CONFIRMED' named entity 'IN S' named entity 'SECRETION' named entity 'RECOMBINANT' named entity 'MONOCLONAL ANTIBODY' named entity 'CONCENTRATION OF' named entity 'TRANSLOCATION' named entity 'S PROTEIN' named entity 'RT-PCR' named entity 'INNATE IMMUNE RESPONSE' named entity 'SPIKE PROTEIN' named entity 'SARS CORONAVIRUS' named entity 'HUMAN MONOCYTE' named entity 'USING' named entity 'TREATMENT' named entity 'CONCENTRATIONS' named entity 'STIMULATED' named entity 'PRODUCTION' named entity 'INCREASES' named entity 'DEPENDENT' named entity 'TPCK' named entity '5.6' named entity 'MRNA' named entity 'IDENTIFIED' named entity '28P' named entity 'THP-1 CELLS' named entity 'SIGNIFICANT' named entity 'ACTIVATION' named entity 'IN VITRO' named entity 'DOSE' named entity 'RAPIDLY' named entity 'ANALYZED' named entity '292' named entity 'FOUND' named entity 'IL-8 PRODUCTION' named entity 'RESPONSES' named entity 'FOLLOWING' named entity 'BAB'

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