About: Development of infectious cDNA clones of Salmonid alphavirus subtype 3

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of infectious cDNA clones of Salmonid alphavirus subtype 3 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of infectious cDNA clones of Salmonid alphavirus subtype 3
Creator	Rimstad, Espen Karlsen, Marius Nylund, Are Ottem, Karl Villoing, Stephane
Source	Medline; PMC
abstract	BACKGROUND: Salmonid alphavirus (SAV) is a widespread pathogen in European aquaculture of salmonid fish. Distinct viral subtypes have been suggested based on sequence comparisons and some of these have different geographical distributions. In Norway, only SAV subtype 3 have so far been identified. Little is known about viral mechanisms important for pathogenesis and transmission. Tools for detailed exploration of SAV genomes are therefore needed. RESULTS: Infectious cDNA clones in which a genome of subtype 3 SAV is under the control of a CMV promoter were constructed. The clones were designed to express proteins that are putatively identical to those previously reported for the SAVH20/03 strain. A polyclonal antiserum was raised against a part of the E2 glycoprotein in order to detect expression of the subgenomic open reading frame (ORF) encoding structural viral proteins. Transfection of the cDNA clone revealed the expression of the E2 protein by IFAT, and in serial passages of the supernatant the presence of infectious recombinant virus was confirmed through RT-PCR, IFAT and the development of a cytopathic effect similar to that seen during infection with wild type SAV. Confirmation that the recovered virus originated from the infectious plasmid was done by sequence identification of an introduced genetic tag. The recombinant virus was infectious also when an additional ORF encoding an EGFP reporter gene under the control of a second subgenomic alphavirus promoter was added. Finally, we used the system to study the effect of selected point mutations on infectivity in Chinook salmon embryo cells. While introduced mutations in nsP2(197), nsP3(263 )and nsP3(323 )severely reduced infectivity, a serine to proline mutation in E2(206 )appeared to enhance the virus titer production. CONCLUSION: We have constructed infectious clones for SAV based on a subtype 3 genome. The clones may serve as a platform for further functional studies.
has issue date	2010-09-21(xsd:dateTime)
bibo:doi	10.1186/1756-0500-3-241
bibo:pmid	20858233
has license	cc-by
sha1sum (hex)	3868d449d1d018addc42aa5f5428a08976a8b190
schema:url	https://doi.org/10.1186/1756-0500-3-241
resource representing a document's title	Development of infectious cDNA clones of Salmonid alphavirus subtype 3
has PubMed Central identifier	PMC2949663
has PubMed identifier	20858233
schema:publication	BMC Res Notes
resource representing a document's body	covid:3868d449d1d018addc42aa5f5428a08976a8b190#body_text
is schema:about of	named entity 'subgenomic' named entity 'clone' named entity 'point mutations' named entity 'glycoprotein' named entity 'embryo' named entity 'mechanisms' named entity 'recombinant virus' named entity 'cDNA clones' named entity 'Development' named entity 'CONSTRUCTED' named entity 'LITTLE' named entity 'VIRAL PROTEINS' named entity 'PATHOGENESIS' named entity 'CONTROL' named entity 'PART OF' named entity 'TRANSMISSION' named entity 'CDNA' named entity 'development' named entity 'encoding' named entity 'mutations' named entity 'serial' named entity 'virus' named entity 'clones' named entity 'IFAT' named entity 'identification' named entity 'suggested' named entity 'genomes' named entity 'recombinant virus' named entity 'nsP2' named entity 'alphavirus' named entity 'aquaculture' named entity 'Transfection' named entity 'EGFP' named entity 'cDNA clone' named entity 'virus' named entity 'ORF' named entity 'cytopathic effect' named entity 'supernatant' named entity 'subgenomic' named entity 'IFAT' named entity 'ligation' named entity 'virus' named entity 'CHSE' named entity 'rate parameter' named entity 'capsid' named entity '197A' named entity 'alphaviruses' named entity 'fluorescence microscopy' named entity 'pathogen' named entity 'vector' named entity 'DNA sequencing' named entity 'Relative solvent accessibility' named entity 'Western blot' named entity 'virus' named entity 'RT-PCR' named entity 'polyA tail' named entity 'conserved region' named entity 'ORF' named entity 'Blue gill' named entity 'Invitrogen' named entity 'glycoprotein' named entity 'cell membrane' named entity 'clone' named entity 'Leica' named entity 'epidemics' named entity 'Transfection' named entity 'recombinant virus' named entity 'evolution' named entity 'cell cultures' named entity '3′UTR' named entity 'sequenced completely' named entity 'titer' named entity 'virus' named entity 'cell cultures' named entity 'clones' named entity 'cell culture' named entity 'tissue tropism'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software