About: Further characterization of the virus-specific RNAs in feline calicivirus infected cells

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About: Further characterization of the virus-specific RNAs in feline calicivirus infected cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Further characterization of the virus-specific RNAs in feline calicivirus infected cells
Creator	Mengeling, William Neil1, John
Source	Elsevier; Medline; PMC
abstract	Abstract The virus-specific RNAs in feline calicivirus (FCV) infected cells were examined to determine the number and forms of RNAs that are synthesized during the infection process. Northern blots of poly(A)+ RNA from 5-h infected cells probed with a cDNA clone derived from the 3' end of the FCV genome (pCV3) revealed four FCV-specific RNAs that were approximately 8.2 (genomic RNA), 4.8, 4.2 and 2.4 kb in length. Northern blots of poly(A)+ RNA purified from infected cells hourly after infection and probed with pCV3 demonstrated that transcription of all FCV-specific RNAs are detectable at 2 to 3 h post-infection (PI) and that these RNAs reached steady state levels at approximately 4 h PI. The levels of the FCV RNAs then remained relatively constant through 7 h PI, the last time tested, with the exception of the 4.8 and 4.2 kb transcripts which showed a marked increase between 6 and 7 hours PI. Northern blots of dsRNA which had been LiCl-fractionated from pooled total cellular RNA isolated from 5-h and 7-h FCV infected cells, showed two double-stranded RNAs corresponding to the 8.2 kb genomic RNA and the 2.4 kb subgenomic RNA. Preliminary mapping by Northern blotting using cDNA probes derived from varying locations within the FCV genome was done to determine the approximate regions from which the subgenomic RNAs are derived. This analysis indicates that the viral RNAs are nested, co-terminal transcripts with common 3' ends.
has issue date	1988-08-31(xsd:dateTime)
bibo:doi	10.1016/0168-1702(88)90067-6
bibo:pmid	3176687
has license	els-covid
sha1sum (hex)	3910c1aeecc74a8209e941e3b78cbac9b9baa4a8
schema:url	https://doi.org/10.1016/0168-1702%2888%2990067-6
resource representing a document's title	Further characterization of the virus-specific RNAs in feline calicivirus infected cells
has PubMed Central identifier	PMC7172345
has PubMed identifier	3176687
schema:publication	Virus Research
resource representing a document's body	covid:3910c1aeecc74a8209e941e3b78cbac9b9baa4a8#body_text
is schema:about of	named entity 'RNAs' named entity 'Further' named entity 'MARKED' named entity 'TRANSCRIPTS' named entity 'DEMONSTRATED' named entity 'END' named entity 'COMMON' named entity 'FORMS' named entity 'NORTHERN BLOTTING' named entity 'APPROXIMATELY' covid:arg/3910c1aeecc74a8209e941e3b78cbac9b9baa4a8 named entity 'infected' named entity 'blots' named entity 'virus-specific' named entity 'RNA' named entity 'detectable' named entity 'marked' named entity 'derived' named entity 'RNAs' named entity 'Northern blots' named entity 'cDNA clone' named entity 'RNAs' named entity 'transcription' named entity 'RNA' named entity '3' ends' named entity 'RNA' named entity 'virus' named entity 'feline calicivirus' named entity '3' end' named entity 'RNAs' named entity '4.2' named entity 'infection' named entity 'virus-specific' named entity 'feline calicivirus' named entity 'double-stranded RNA' named entity 'DNA polymerase' named entity 'Autoradiography' named entity 'cDNA clone' named entity 'RNase' named entity 'double-stranded' named entity 'reverse transcription' named entity 'RNA' named entity 'reverse transcriptase' named entity 'chromatography' named entity 'oligonucleotide' named entity 'RNA' named entity 'adsorb' named entity 'hybridization' named entity 'cDNA' named entity 'centrifugation' named entity 'RNA' named entity 'oligonucleotide' named entity 'glycerol' named entity 'chromatography' named entity 'X-gal' named entity 'double-stranded' named entity 'RNase' named entity 'cDNA library' named entity '3' end' named entity 'coronaviruses' named entity 'chain termination' named entity 'PBS' named entity 'cDNA' named entity 'virus' named entity 'inoculum' named entity 'centrifugation' named entity 'methylase' named entity 'phosphate buffered saline' named entity 'DNA polymerase' named entity 'subgenomic RNAs' named entity 'sequenced' named entity 'IPTG' named entity 'double-stranded RNA' named entity 'E. coli' named entity 'Luria broth' named entity 'nitrocellulose' named entity 'double-stranded RNA' named entity 'fetal calf serum' named entity 'RNase' named entity 'polypeptides'

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