About: FN3 protein fragment containing two type III fibronectin domains from B. longum GT15 binds to human tumor necrosis factor alpha in vitro

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About: FN3 protein fragment containing two type III fibronectin domains from B. longum GT15 binds to human tumor necrosis factor alpha in vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	FN3 protein fragment containing two type III fibronectin domains from B. longum GT15 binds to human tumor necrosis factor alpha in vitro
Creator	Alekseeva, Maria Bushkova, Kristina Chernyshova, Irina Danilenko, Valery Dyachkova, Marina Dyakov, Ilya Gavrilova, Marina Mavletova, Dilara Snegireva, Nadezda
Source	Elsevier; Medline; PMC
abstract	Most species of the genus Bifidobacterium contain the gene cluster PFNA, which is presumably involved in the species-specific communication between bacteria and their hosts. The gene cluster PFNA consists of five genes including fn3, which codes for a protein containing two fibronectin type III domains. Each fibronectin domain contains sites similar to cytokine-binding sites of human receptors. Based on this finding we assumed that this protein would bind specifically to human cytokines in vitro. We cloned a fragment of the fn3 gene (1503 bp; 501 aa) containing two fibronectin domains, from the strain B. longum subsp. longum GT15. After cloning the fragment into the expression vector pET16b and expressing it in E. coli, the protein product was purified to a homogenous state for further analysis. Using the immunoferment method, we tested the purified fragment's ability to bind the following human cytokines: IL-1β, IL-6, IL-10, TNFα. We developed a sandwich ELISA system to detect any specific interactions between the purified protein and any of the studied cytokines. We found that the purified protein fragment only binds to TNFα.
has issue date	2020-08-06(xsd:dateTime)
bibo:doi	10.1016/j.anaerobe.2020.102247
bibo:pmid	32771620
has license	no-cc
sha1sum (hex)	3973dee0a10700bcbb31bf7506462d254bf4aaf6
schema:url	https://doi.org/10.1016/j.anaerobe.2020.102247
resource representing a document's title	FN3 protein fragment containing two type III fibronectin domains from B. longum GT15 binds to human tumor necrosis factor alpha in vitro
has PubMed Central identifier	PMC7409735
has PubMed identifier	32771620
schema:publication	Anaerobe
resource representing a document's body	covid:3973dee0a10700bcbb31bf7506462d254bf4aaf6#body_text
is schema:about of	named entity 'tumor necrosis factor alpha' named entity 'human' named entity 'fragment' named entity 'domains' named entity 'human' named entity 'fragment' named entity 'Journal' named entity 'tumor necrosis factor alpha' named entity 'protein' named entity 'B. longum' named entity 'tumor necrosis factor alpha' named entity 'domains' named entity 'nucleotide' named entity 'cytokines' named entity 'active site' named entity 'cytokines' named entity 'host organism' named entity 'solid phase' named entity 'protein' named entity 'antigen' named entity 'similar in structure' named entity 'isoelectric point' named entity 'Bio-Rad' named entity 'protein' named entity 'cytokine' named entity 'PMSF' named entity 'operon' named entity 'Qubit' named entity 'PBS buffer' named entity 'polyclonal' named entity 'adsorbed' named entity 'host organism' named entity 'antibody' named entity 'TNFα' named entity 'cytokines' named entity 'operon' named entity 'non-specific' named entity 'PMSF' named entity 'protein' named entity 'non-specific' named entity 'Optical density' named entity 'antibodies' named entity 'interact' named entity 'bifidobacteria' named entity '2-mercaptoethanol' named entity 'adsorption' named entity 'PMSF' named entity 'Optical density' named entity 'carbonate' named entity 'lysate' named entity 'protein' named entity 'PFNA' named entity 'polystyrene' named entity 'IL-6' named entity 'human body' named entity 'Optical density' named entity 'protein' named entity 'TNFα' named entity 'evolution' named entity 'Insoluble' named entity 'lysozyme' named entity 'cytokine' named entity 'protein' named entity 'Invitrogen' named entity 'cytokines' named entity 'B. longum' named entity 'chromatography' named entity 'pH 8' named entity 'lysate' named entity 'protein' named entity 'UniProt' named entity 'cytokines' named entity 'bifidobacteria'

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