About: DEVELOPMENT OF AN ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA) FOR ANALYSIS OF LISTERIOLYSIN O PRODUCED BY LISTERIA MONOCYTOGENES

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About: DEVELOPMENT OF AN ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA) FOR ANALYSIS OF LISTERIOLYSIN O PRODUCED BY LISTERIA MONOCYTOGENES Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	DEVELOPMENT OF AN ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA) FOR ANALYSIS OF LISTERIOLYSIN O PRODUCED BY LISTERIA MONOCYTOGENES
Creator	Kim, Kee-Tae Murano3, Elsa Olson4, Dennis
Source	PMC
abstract	Listeriolysin O (LLO) is a heat‐labile hemolysin produced by Listeria mono‐cytogenes. Its hemolytic activity has been evaluated qualitatively by sodium dodecyl sulfate (SDS) electrophoresis and immunoblotting. In this experiment, an enzyme‐linked immunosorbent assay (ELISA) was developed for quantitative analysis of LLO by using Streptolysin O (SLO) and antistreptolysin O (ASO) as the reagents. The selected coating and blocking buffers were 0.05 M Tris buffer (pH 8.5) and 0.25% casein solution with phosphate‐buffered saline solution + 0.05% Tween 20 (PBS‐T), respectively. A relationship between ASO and antibody was achieved with 5 mg/ml ASO and a 1:1,000 dilution of conjugate. The heat stability of LLO at 48, 62, 72, and 80C was examined by using this method and compared with a traditional hemolysis assay. Although the LLO is inactivated easily at those temperatures, the protein structure was not affected at temperatures lower than 80C for 3 min, pointing to a need for both hemolysis and ELISA to be conducted in determining both the activity and presence of LLO in foods.
has issue date	2007-05-05(xsd:dateTime)
bibo:doi	10.1111/j.1745-4581.1993.tb00289.x
has license	no-cc
sha1sum (hex)	39bb5b2188ff5a76ebf43512d437e321d9a238fc
schema:url	https://doi.org/10.1111/j.1745-4581.1993.tb00289.x
resource representing a document's title	DEVELOPMENT OF AN ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA) FOR ANALYSIS OF LISTERIOLYSIN O PRODUCED BY LISTERIA MONOCYTOGENES
has PubMed Central identifier	PMC7159331
schema:publication	J Rapid Methods Autom Microbiol
resource representing a document's body	covid:39bb5b2188ff5a76ebf43512d437e321d9a238fc#body_text
is schema:about of	named entity 'FOODS' named entity 'CONJUGATE' named entity 'ELECTROPHORESIS' named entity '0.05' named entity 'TRIS BUFFER' named entity 'COATING' named entity 'ACTIVITY' named entity 'LOWER' named entity 'hemolysis' named entity 'Tris buffer' named entity 'immunosorbent assay' named entity 'Streptolysin' named entity 'hemolysis' named entity 'SLO' named entity 'linear relationship' named entity 'glucose' named entity 'enzymatic activity' named entity 'antibody' named entity 'optical density' named entity 'LLO' named entity 'antibody' named entity 'red blood cells' named entity 'enzyme' named entity 'monoclonal antibodies' named entity 'LLO' named entity 'dialyzed' named entity 'denatured' named entity 'sodium thiosulfate' named entity 'SLO' named entity 'room temperature' named entity 'antibody' named entity 'conjugated' named entity 'hemolysis' named entity 'reagent' named entity '5.0' named entity 'milliliter' named entity 'human blood' named entity 'optical density' named entity 'Houston' named entity 'Difco' named entity 'standard curve' named entity 'LLO' named entity 'red blood cell' named entity '5.0' named entity 'LLO' named entity 'protein' named entity 'distilled water' named entity 'microtiter plate' named entity 'Tris buffer' named entity 'protein' named entity 'streptolysin' named entity 'water-soluble' named entity 'distilled water' named entity 'antibody' named entity 'pH 8' named entity 'LLO' named entity 'heat stability' named entity 'pH 8' named entity 'precipitate' named entity '5.0' named entity '0.45' named entity 'bacteria' named entity 'enzymatic reaction' named entity 'lyophilized' named entity 'heat treatment' named entity 'cellulose acetate' named entity 'antibody' named entity 'hemolysis' named entity 'antigen' named entity 'alkaline phosphatase' named entity 'conjugated' named entity 'immunoglobulin' named entity 'St. Louis' named entity 'protein' named entity 'vacuole' named entity 'virulence' named entity 'nonspecific binding' named entity 'antibodies' named entity 'centrifuged' named entity 'Tris' named entity 'casein'

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