About: De novo transcriptomic analysis of light-induced flavonoid pathway, transcription factors in the flower buds of Lonicera japonica

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: De novo transcriptomic analysis of light-induced flavonoid pathway, transcription factors in the flower buds of Lonicera japonica Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	De novo transcriptomic analysis of light-induced flavonoid pathway, transcription factors in the flower buds of Lonicera japonica
Creator	Li, Yiming Liu, · Liang, Chengyuan Qi, · Xu, · Fang, Hailing Li, Weilin Xu, Dongbei
source	PMC
abstract	KEY MESSAGE: Transcriptomic analysis of the relationship between gene expression patterns and flavonoid contents in the flower buds of Lonicera japonica under light-induced conditions, especially the flavonoid pathway genes and transcription factors. ABSTRACT: Flos Lonicerae Japonicae (FLJ), the flower buds of Lonicera japonica Thunb., has been used to treat some human diseases including severe respiratory syndromes and hand-foot-and-mouth diseases owing to its putative antibacterial, and antiviral effects. Luteoloside is a flavonoid that is used by the Chinese Pharmacopoeia to evaluate the quality of FLJ. Light is an important environmental factor that affects flavonoid biosynthesis in the flower buds of L. japonica. However, how light triggers increases in flavonoid production remains unclear. To enhance our understanding of the mechanism involved in light-regulated flavonoid biosynthesis, we sequenced the transcriptomes of L. japonica exposed to three different light conditions: 100% light intensity (CK), 50% light intensity (LI50), and 25% light intensity (LI25) using an Illumina HiSeq 4000 System. A total of 77,297 unigenes with an average length of 809 bp were obtained. Among them, 43,334 unigenes (56.06%) could be matched to at least one biomolecular database. Additionally, 4188, 1545 and 1023 differentially expressed genes (DEGs) were identified by comparative transcriptomics LI25-vs-CK, LI50-vs-CK, and LI25-vs-LI50, respectively. Of note, genes known to be involved in flavonoid biosynthesis, such as 4-coumarate coenzyme A ligase (4CL), and chalcone synthase (CHS) were up-regulated. In addition, a total of 1649 transcription factors (TFs) were identified and divided into 58 TF families; 98 TFs exhibited highly dynamic changes in response to light intensity. Quantitative real-time PCR (qRT-PCR) was used to test the expression profiles of the RNA sequencing (RNA-Seq) data. This study offers insight into how transcriptional expression pattern is influenced by light in the flower buds of L. japonica, and will enhance the understanding of molecular mechanisms of flavonoid biosynthesis in response to light in L. japonica. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00468-019-01916-4) contains supplementary material, which is available to authorized users.
has issue date	2019-11-02(xsd:dateTime)
bibo:doi	10.1007/s00468-019-01916-4
bibo:pmid	32435087
has license	no-cc
sha1sum (hex)	3a876a0d4c375855472e81e3084061f9459348ce
schema:url	https://doi.org/10.1007/s00468-019-01916-4
resource representing a document's title	De novo transcriptomic analysis of light-induced flavonoid pathway, transcription factors in the flower buds of Lonicera japonica
has PubMed Central identifier	PMC7223627
has PubMed identifier	32435087
schema:publication	Trees (Berl West)
resource representing a document's body	covid:3a876a0d4c375855472e81e3084061f9459348ce#body_text
is schema:about of	named entity 'conditions' named entity 'Lonicera' named entity 'transcriptomic' named entity 'Transcriptomic' named entity 'flower buds' named entity 'flower buds' named entity 'transcription factors' named entity 'flower buds' named entity 'ERF' named entity 'biosynthesis' named entity 'MYB' named entity 'expression levels' named entity 'WRKY' named entity 'MYB' named entity 'expressed genes' named entity 'RNA' named entity 'reverse transcription' named entity 'flower buds' named entity 'Genetic Information' named entity 'gene expression' named entity 'dATP' named entity 'exposed to light' named entity 'Cellular Processes' named entity 'unigenes' named entity 'expression levels' named entity 'MYB' named entity 'Premier 5' named entity 'unigenes' named entity 'L. japonica' named entity 'MYB' named entity 'Qiagen' named entity 'RNA sequencing' named entity 'bZIP' named entity 'physiological processes' named entity 'environmental factors' named entity 'Chinese Pharmacopoeia' named entity 'Actin' named entity 'Illumina' named entity 'flower buds' named entity 'anti-viral' named entity 'KOG' named entity 'Flavonol synthase' named entity 'rutinoside' named entity 'secondary metabolites' named entity 'metabolic' named entity 'flavonoids' named entity 'down-regulated' named entity 'Carbapenem' named entity 'up-regulated' named entity 'KEGG' named entity 'RNA-seq' named entity 'Sequence Database' named entity 'biosynthesis' named entity 'Pyrus pyrifolia' named entity 'honeysuckle' named entity 'Nanjing' named entity 'RNA-Seq' named entity 'Orthologous' named entity 'molecular mechanism' named entity 'flavonoid' named entity 'Carbohydrate metabolism' named entity 'transcriptome' named entity 'MYB' named entity 'unigenes' named entity 'unigenes' named entity 'hyperlipidemia' named entity 'Monoterpenoid' named entity 'transcription factors' named entity 'KEGG' named entity 'bZIP' named entity 'molecular mechanism' named entity 'eukaryotic' named entity 'Shang' named entity 'MYB'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software