About: Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

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About: Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43
Creator	Nakauchi, Mina Kariwa, Hiroaki Baek, Min-Won Heo, Suk-Jin Kim, Dong-Jae Lee, Byoung-Hee Lee, Hui-Young Lee, Hyun-Kyoung Na, Yi-Rang Noh, Kyoung-Jin Park, Jae-Hak Park, Sung-Hoon Seok, Seung-Hyeok Noton Kumar, Dutta
Source	Medline; PMC
abstract	Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.
has issue date	2010-05-11(xsd:dateTime)
bibo:doi	10.4142/jvs.2010.11.2.165
bibo:pmid	20458159
has license	no-cc
sha1sum (hex)	3ac3823b31229b9754a336bf17f5ff76fdcaadf2
schema:url	https://doi.org/10.4142/jvs.2010.11.2.165
resource representing a document's title	Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43
has PubMed Central identifier	PMC2873818
has PubMed identifier	20458159
schema:publication	J Vet Sci
resource representing a document's body	covid:3ac3823b31229b9754a336bf17f5ff76fdcaadf2#body_text
is schema:about of	named entity 'ANTIBODIES' named entity 'nucleocapsid' named entity 'antibodies' named entity 'PRODUCTION' named entity 'CORONAVIRUS' named entity 'OC43' named entity 'ABS' named entity 'OC43' named entity 'BUT' named entity 'SARS' named entity 'CORONAVIRUSES' named entity 'NUCLEOCAPSID PROTEIN' named entity 'RECOMBINANT' named entity 'THESE' named entity 'CROSS REACTIVITY' named entity '229E' named entity 'SPECIFIC' named entity 'CROSS' named entity 'SARS' named entity 'WESTERN BLOTTING' named entity 'HUMAN CORONAVIRUS' named entity 'HUMAN' named entity '229E' named entity 'SARS VIRUS' named entity 'NUCLEOCAPSID PROTEIN' named entity 'REACTIVITY' named entity 'DETERMINED BY' named entity 'PROTEIN ' covid:arg/3ac3823b31229b9754a336bf17f5ff76fdcaadf2 named entity 'Western blotting' named entity 'specific' named entity 'Western blotting' named entity 'SARS' named entity 'OC43' named entity 'cross reactivity' named entity 'recombinant' named entity 'SARS' named entity 'nucleocapsid' named entity 'IPTG' named entity 'protein' named entity 'Epidemiology' named entity 'Cross-reactivity' named entity 'cross-reactivity' named entity 'nucleocapsid' named entity 'nucleocapsid' named entity 'nucleocapsid' named entity 'Shang' named entity 'motif' named entity 'Origami' named entity 'recombinant' named entity 'cell lysates' named entity 'Korea' named entity 'highly conserved' named entity 'cDNA' named entity 'nucleocapsid' named entity 'antigenicity' named entity 'SARS' named entity 'IgG2a' named entity 'motif' named entity 'viruses' named entity 'porcine' named entity 'sensitive and specific' named entity 'polyclonal' named entity 'HCoV NL63' named entity 'SARS-CoV' named entity 'nucleocapsid' named entity 'Western blotting' named entity 'Bioneer'

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