About: Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)

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About: Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)
Creator	Zhang, Yan Fan, Quanshui Wang, Yiyin Zhang, Fuqiang Feng, Ziliang Hu, Tingsong Qiu, Wei Yu, Jing Zheng, Ying Zhou, Weiguo Cui, Qinghua Li, Gangshan Zhang, Chaoxiong Zhou, Xiaofang
Source	PMC
abstract	BACKGROUND: The identification of new virus strains is important for the study of infectious disease, but current (or existing) molecular biology methods are limited since the target sequence must be known to design genome-specific PCR primers. Thus, we developed a new method for the discovery of unknown viruses based on the cDNA - random amplified polymorphic DNA (cDNA-RAPD) technique. Getah virus, belonging to the family Togaviridae in the genus Alphavirus, is a mosquito-borne enveloped RNA virus that was identified using the Virus-Discovery-cDNA RAPD (VIDISCR) method. RESULTS: A novel Getah virus was identified by VIDISCR from suckling mice exposed to mosquitoes (Aedes albopictus) collected in Yunnan Province, China. The non-structural protein gene, nsP3, the structural protein gene, the capsid protein gene, and the 3'-untranslated region (UTR) of the novel Getah virus isolate were cloned and sequenced. Nucleotide sequence identities of each gene were determined to be 97.1–99.3%, 94.9–99.4%, and 93.6–99.9%, respectively, when compared with the genomes of 10 other representative strains of Getah virus. CONCLUSIONS: The VIDISCR method was able to identify known virus isolates and a novel isolate of Getah virus from infected mice. Phylogenetic analysis indicated that the YN08 isolate was more closely related to the Hebei HB0234 strain than the YN0540 strain, and more genetically distinct from the MM2021 Malaysia primitive strain.
has issue date	2012-12-27(xsd:dateTime)
bibo:doi	10.1186/1471-2180-12-305
bibo:pmid	23268691
has license	cc-by
sha1sum (hex)	3acc047295ee11b02f13aac787a12fe114d58558
schema:url	https://doi.org/10.1186/1471-2180-12-305
resource representing a document's title	Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)
has PubMed Central identifier	PMC3547691
has PubMed identifier	23268691
schema:publication	BMC Microbiol
resource representing a document's body	covid:3acc047295ee11b02f13aac787a12fe114d58558#body_text
is schema:about of	named entity 'RELATED' named entity 'BORNE' named entity 'TOGAVIRIDAE' named entity 'GENOME' named entity 'OF EACH' named entity 'IDENTIFIED' named entity 'YUNNAN PROVINCE' named entity 'CONCLUSIONS' named entity 'UNKNOWN' named entity 'NEW' named entity 'MOSQUITO' named entity 'DISTINCT FROM' named entity 'STUDY' named entity 'BASED' named entity 'FAMILY' named entity 'UNTRANSLATED REGION' named entity 'REPRESENTATIVE' named entity 'POLYMORPHIC' named entity 'NOVEL' named entity 'VIRUS' named entity 'STRAINS' named entity 'AMPLIFIED' named entity 'RANDOM' named entity 'genomes' named entity 'sequenced' named entity 'genetically' named entity 'mosquitoes' named entity 'CAPSID PROTEIN' named entity 'IMPORTANT' named entity 'DISCOVERY' named entity 'BELONGING' named entity 'KNOWN' named entity 'CDNA' named entity 'MALAYSIA' named entity 'CURRENT' named entity 'PROTEIN GENE' named entity 'BUT' named entity 'MOSQUITOES' named entity 'GETAH VIRUS' named entity 'DETERMINED' named entity 'COMPARED' named entity 'AMPLIFIED' named entity 'NON-' named entity 'NUCLEOTIDE SEQUENCE' named entity 'MICE' named entity 'LIMITED' named entity '99.9' named entity 'INFECTED' named entity 'TARGET' named entity 'RANDOM' named entity 'GETAH VIRUS' named entity 'CDNA' named entity 'IDENTIFICATION' named entity 'DNA' named entity 'NOVEL' named entity 'VIRUS' named entity 'ALPHAVIRUS' named entity 'PCR PRIMERS' named entity 'INFECTIOUS DISEASE' named entity 'IS A' named entity 'PRIMITIVE' named entity 'RAPD' named entity 'MOLECULAR BIOLOGY METHODS' named entity 'PHYLOGENETIC ANALYSIS' named entity 'METHOD' named entity 'TECHNIQUE' named entity 'RNA VIRUS' named entity 'COLLECTED'

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