About: A novel mosquito ubiquitin targets viral envelope protein for degradation and reduces virion production during dengue virus infection

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About: A novel mosquito ubiquitin targets viral envelope protein for degradation and reduces virion production during dengue virus infection Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A novel mosquito ubiquitin targets viral envelope protein for degradation and reduces virion production during dengue virus infection
Creator	Higgs, Stephen Vanlandingham, Dana Fikrig, Erol Cloherty, Erin Jameson, Samuel Londono-Renteria, Berlin Troupin, Andrea Colpitts, Tonya Conway, Michael
topic	covid:3ba4af47b206aeaee597ce7c4f4bf27db05ae87c#this
Source	Elsevier; Medline; PMC
abstract	Abstract Background Dengue virus (DENV) is a mosquito-borne flavivirus that causes significant human disease and mortality in the tropics and subtropics. By examining the effects of virus infection on gene expression, and interactions between virus and vector, new targets for prevention of infection and novel treatments may be identified in mosquitoes. We previously performed a microarray analysis of the Aedes aegypti transcriptome during infection with DENV and found that mosquito ubiquitin protein Ub3881 (AAEL003881) was specifically and highly down-regulated. Ubiquitin proteins have multiple functions in insects, including marking proteins for proteasomal degradation, regulating apoptosis and mediating innate immune signaling. Methods We used qRT-PCR to quantify gene expression and infection, and RNAi to reduce Ub3881 expression. Mosquitoes were infected with DENV through blood feeding. We transfected DENV protein expression constructs to examine the effect of Ub3881 on protein degradation. We used site-directed mutagenesis and transfection to determine what amino acids are involved in Ub3881-mediated protein degradation. Immunofluorescence, Co-immunoprecipitation and Western blotting were used to examine protein interactions and co-localization. Results The overexpression of Ub3881, but not related ubiquitin proteins, decreased DENV infection in mosquito cells and live Ae. aegypti. The Ub3881 protein was demonstrated to be involved in DENV envelope protein degradation and reduce the number of infectious virions released. Conclusions We conclude that Ub3881 has several antiviral functions in the mosquito, including specific viral protein degradation. General significance Our data highlights Ub3881 as a target for future DENV prevention strategies in the mosquito transmission vector.
has issue date	2016-09-30(xsd:dateTime)
bibo:doi	10.1016/j.bbagen.2016.05.033
bibo:pmid	27241849
has license	els-covid
sha1sum (hex)	3ba4af47b206aeaee597ce7c4f4bf27db05ae87c
schema:url	https://doi.org/10.1016/j.bbagen.2016.05.033
resource representing a document's title	A novel mosquito ubiquitin targets viral envelope protein for degradation and reduces virion production during dengue virus infection
has PubMed Central identifier	PMC4949077
has PubMed identifier	27241849
schema:publication	Biochimica et Biophysica Acta (BBA) - General Subjects
resource representing a document's body	covid:3ba4af47b206aeaee597ce7c4f4bf27db05ae87c#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/3ba4af47b206aeaee597ce7c4f4bf27db05ae87c
is schema:about of	named entity 'Dengue virus' named entity 'transfection' named entity 'involved' named entity 'released' named entity 'Aedes aegypti' named entity 'overexpression' named entity 'infection' named entity 'targets' named entity 'functions' named entity 'feeding' named entity 'highly' named entity 'regulating' named entity 'licenses' named entity 'http' named entity 'degradation' named entity 'targets' named entity 'MEDIATED' named entity 'PROTEIN ' named entity 'TARGETS' named entity 'EXPRESSION' named entity 'METHODS' named entity 'TRANSMISSION' named entity 'TO REDUCE' covid:arg/3ba4af47b206aeaee597ce7c4f4bf27db05ae87c named entity 'gene' named entity 'protein degradation' named entity 'involved' named entity 'data' named entity 'mosquito' named entity 'disease' named entity 'virions' named entity 'Methods' named entity 'constructs' named entity 'Coimmunoprecipitation' named entity 'infection' named entity 'Western blotting' named entity 'interactions' named entity 'flavivirus' named entity 'DENV' named entity 'amino acids' named entity 'innate immune signaling' named entity 'mosquito' named entity 'DENV' named entity 'protein degradation' named entity 'viral protein' named entity 'infection' named entity 'mosquito' named entity 'ubiquitin' named entity 'protein' named entity 'microarray analysis' named entity 'Immunofluorescence' named entity 'Dengue virus' named entity 'infection' named entity 'vector' named entity 'virion' named entity 'ubiquitin' named entity 'performed' named entity 'infection' named entity 'Imd' named entity 'pathogenesis' named entity 'virions' named entity 'DENV' named entity 'mosquito' named entity 'RNA' named entity 'Cell lysates' named entity 'precipitate' named entity 'mosquito' named entity 'paraformaldehyde' named entity 'protein' named entity 'human papillomavirus' named entity 'lysate'

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