About: Localization of Low-Density Detergent-Resistant Membrane Proteins in Intact and Acrosome-Reacted Mouse Sperm

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About: Localization of Low-Density Detergent-Resistant Membrane Proteins in Intact and Acrosome-Reacted Mouse Sperm Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Localization of Low-Density Detergent-Resistant Membrane Proteins in Intact and Acrosome-Reacted Mouse Sperm
Creator	Allaire, Alicia Sosnik, Julian Miranda, Patricia Visconti, Pablo
Source	PMC
abstract	Mammalian sperm become fertile after completing capacitation, a process associated with cholesterol loss and changes in the biophysical properties of the sperm membranes that prepares the sperm to undergo the acrosome reaction. Different laboratories have hypothesized that cholesterol efflux can influence the extent and/or movement of lipid raft microdomains. In a previous study, our laboratory investigated the identity of sperm proteins putatively associated with rafts. After extraction with Triton X-100 and ultracentrifugation in sucrose gradients, proteins distributing to the light buoyant-density fractions were cored from polyacrylamide gels and microsequenced. In this study, a subset of these proteins (TEX101, basigin, hexokinase 1, facilitated glucose transporter 3, IZUMO, and SPAM1) and other molecules known to be enriched in membrane rafts (caveolin 2, flotillin 1, flotillin 2, and the ganglioside GM3) were selected to investigate their localization in the sperm and their behavior during capacitation and the acrosome reaction. These molecules localize to multiple sperm domains, including the acrosomal cap (IZUMO, caveolin 2, and flotillin 2), equatorial segment (GM3), cytoplasmic droplet (TEX101), midpiece (basigin, facilitated glucose transporter 3, and flotillin 2), and principal piece (facilitated glucose transporter 3). Some of these markers modified their immunofluorescence pattern after sperm incubation under capacitating conditions, and these changes correlated with the occurrence of the acrosome reaction. While GM3 and caveolin 2 were not detected after the acrosome reaction, flotillin 2 was found in the equatorial segment of acrosome-reacted sperm, and IZUMO distributed along the sperm head, reaching the post- and para-acrosomal areas. Taking into consideration the requirement of the acrosome reaction for sperm to become fusogenic, these results suggest that membrane raft dynamics may have a role in sperm-egg membrane interaction.
has issue date	2009-05-01(xsd:dateTime)
bibo:doi	10.1095/biolreprod.108.075242
bibo:pmid	19144954
has license	bronze-oa
sha1sum (hex)	3cbc71c13d33818568327dbe07aba209549aea2a
schema:url	https://doi.org/10.1095/biolreprod.108.075242
resource representing a document's title	Localization of Low-Density Detergent-Resistant Membrane Proteins in Intact and Acrosome-Reacted Mouse Sperm
has PubMed Central identifier	PMC2804839
has PubMed identifier	19144954
schema:publication	Biology of Reproduction
resource representing a document's body	covid:3cbc71c13d33818568327dbe07aba209549aea2a#body_text
is schema:about of	named entity 'principal' named entity 'acrosome reaction' named entity 'immunofluorescence' named entity 'raft' named entity 'acrosome reaction' named entity 'sperm' named entity 'gels' named entity 'localize' named entity 'IZUMO' named entity 'LOW-DENSITY' named entity 'EQUATORIAL' named entity 'BEHAVIOR' named entity 'ACROSOME' named entity 'SUCROSE' named entity 'PREVIOUS' named entity 'DENSITY' named entity 'DIFFERENT' named entity 'FERTILE' named entity 'MULTIPLE' named entity 'GANGLIOSIDE GM3' named entity 'FLOTILLIN 2' named entity 'CORED' named entity 'CONSIDERATION' named entity 'TAKING' named entity 'INTERACTION' named entity 'THEIR' named entity 'AREAS' named entity 'INCLUDING' named entity 'DROPLET' named entity 'MARKERS' named entity 'SPAM1' named entity 'EGG MEMBRANE' named entity 'EXTENT' named entity 'SPERM' named entity 'PIECE' named entity 'HEXOKINASE 1' named entity 'RESULTS' named entity 'CYTOPLASMIC' named entity 'MEMBRANE PROTEINS' named entity 'LOSS' named entity 'HAVE' named entity 'CAVEOLIN 2' named entity 'POLYACRYLAMIDE GELS' named entity 'PATTERN' named entity 'SELECTED' named entity 'GRADIENTS' named entity 'PROCESS' named entity 'SUBSET' named entity 'SPERM' named entity 'ACROSOME' named entity 'FOUND' named entity 'CHOLESTEROL EFFLUX' named entity 'MEMBRANES' named entity 'IMMUNOFLUORESCENCE' named entity 'POST' named entity 'EXTRACTION' named entity 'PRINCIPAL' named entity 'DYNAMICS' named entity 'ACROSOME REACTION' named entity 'LABORATORY' named entity 'MAY HAVE' named entity 'ULTRACENTRIFUGATION' named entity 'GM3' named entity 'KNOWN' named entity 'LIPID RAFT' named entity 'CHOLESTEROL' named entity 'MOLECULES' named entity 'LOCALIZATION' named entity 'IZUMO' named entity 'INCUBATION' named entity 'TO INVESTIGATE' named entity 'IDENTITY' named entity 'PARA' named entity 'LOCALIZE' named entity 'MIDPIECE' named entity 'LABORATORIES' named entity 'INVESTIGATED' named entity 'GLUCOSE TRANSPORTER 3'

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