About: Mutational analysis of a helicase motif-based RNA 5′-triphosphatase/NTPase from bamboo mosaic virus

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About: Mutational analysis of a helicase motif-based RNA 5′-triphosphatase/NTPase from bamboo mosaic virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mutational analysis of a helicase motif-based RNA 5′-triphosphatase/NTPase from bamboo mosaic virus
Creator	Hsu, Yau-Heiu Meng, Menghsiao Chen, Ya-Chio Han, Yu-Tsung Lin, Ming-Kuem Tsai, Chia-Sheng
Source	Elsevier; Medline; PMC
abstract	Abstract The helicase-like domain of BaMV replicase possesses NTPase and RNA 5′-triphosphatase activities. In this study, mutational effects of the helicase signature motifs and residue L543 on the two activities were investigated. Either activity was inactivated by K643A-S644A, D702A, D730A, R855A, or L543P mutations. On the other hand, Q826A, D858A and L543A had activities, in terms of k cat/K m, reduced by 5- to 15-fold. AMPPNP, a nonhydrolyzable ATP analogue, competitively inhibited RNA 5′-triphosphatase activity. Analogies of mutational effects on the two activities and approximation of K i(AMPPNP) and K m(ATP) suggest that the catalytic sites of the activities are overlapped. Mutational effects on the viral accumulation in Chenopodium quinoa indicated that the activities manifested by the domain are required for BaMV survival. Results also suggest that Q826 in motif V plays an additional role in preventing tight binding to ATP, which would otherwise decrease further RNA 5′-triphosphatase, leading to demise of the virus in plant.
has issue date	2007-10-10(xsd:dateTime)
bibo:doi	10.1016/j.virol.2007.05.013
bibo:pmid	17585982
has license	els-covid
sha1sum (hex)	3d388d1f009d319c497ff11412c9ce3aee6b1abf
schema:url	https://doi.org/10.1016/j.virol.2007.05.013
resource representing a document's title	Mutational analysis of a helicase motif-based RNA 5′-triphosphatase/NTPase from bamboo mosaic virus
has PubMed Central identifier	PMC7103348
has PubMed identifier	17585982
schema:publication	Virology
resource representing a document's body	covid:3d388d1f009d319c497ff11412c9ce3aee6b1abf#body_text
is schema:about of	named entity 'RNA' named entity 'INDICATED' named entity 'INHIBITED' named entity 'CHENOPODIUM QUINOA' named entity 'MUTATIONS' named entity 'POSSESSES' named entity 'REDUCED' named entity 'TIGHT' named entity 'EITHER' named entity 'PREVENTING' named entity 'BINDING' named entity 'LIKE' named entity 'MANIFESTED BY' named entity 'DECREASE' named entity '80%' named entity 'TERMS' named entity 'ANALOGIES' named entity 'MOTIFS' named entity 'OVERLAPPED' named entity 'TRIPHOSPHATASE ACTIVITY' named entity 'INACTIVATED' named entity 'CAT' named entity 'STUDY' named entity 'HELICASE' named entity 'BAMBOO MOSAIC VIRUS' named entity 'MUTATIONAL ANALYSIS' named entity 'BASED' named entity 'MOTIF' named entity 'REPLICASE' named entity 'RESIDUE' named entity 'APPROXIMATION' named entity 'EFFECTS' named entity 'TRIPHOSPHATASE' named entity 'ACCUMULATION' named entity 'REQUIRED' named entity 'CATALYTIC' named entity 'MOTIF' named entity 'NTPASE' named entity 'ACTIVITY' named entity 'RESULTS' named entity 'HAND' named entity '80%' named entity 'DOMAIN' named entity 'ANALOGUE' named entity 'SITES' named entity 'ROLE' named entity 'HELICASE' named entity 'PLAYS' named entity 'RNA' named entity 'ADDITIONAL' named entity 'LEADING' named entity 'PLANT' named entity 'TRIPHOSPHATASE' named entity 'ATP' named entity 'SUGGEST' named entity 'SIGNATURE' named entity 'FOLD' named entity 'INVESTIGATED' named entity 'AMPPNP' named entity 'ACTIVITIES' named entity 'VIRAL' named entity 'VIRUS' named entity 'SURVIVAL' covid:arg/3d388d1f009d319c497ff11412c9ce3aee6b1abf named entity 'sites' named entity 'possesses' named entity 'RNA' named entity 'The' named entity 'domain' named entity 'RNA' named entity 'RNA' named entity 'helicase' named entity 'motif' named entity 'RNA' named entity 'incubation' named entity 'glutamine'

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