About: Quantitative Proteomic Analysis of Duck Ovarian Follicles Infected with Duck Tembusu Virus by Label-Free LC-MS

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About: Quantitative Proteomic Analysis of Duck Ovarian Follicles Infected with Duck Tembusu Virus by Label-Free LC-MS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Quantitative Proteomic Analysis of Duck Ovarian Follicles Infected with Duck Tembusu Virus by Label-Free LC-MS
Creator	Yang, Jing Han, Kaikai Huang, Xinmei Li, Yin Liu, Qingtao Liu, Yuzhuo Zhao, Dongmin Liu, Zhao An, Fengjiao Zhang, Dabing Lu, Wuxun Adachi, Akio
Source	Medline; PMC
abstract	Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. DTMUV infection mainly results in significant decreases in egg production in egg-laying ducks within 1–2 weeks post infection. However, information on the comparative protein expression of host tissues in response to DTMUV infection is limited. In the present study, the cellular protein response to DTMUV infection in duck ovarian follicles was analyzed using nano-flow high-performance liquid chromatography-electrospray tandem mass spectrometry. Quantitative proteomic analysis revealed 131 differentially expressed proteins, among which 53 were up regulated and 78 were down regulated. The identified proteins were involved in the regulation of essential processes such as cellular structure and integrity, RNA processing, protein biosynthesis and modification, vesicle transport, signal transduction, and mitochondrial pathway. Some selected proteins that were found to be regulated in DTMUV-infected tissues were screened by quantitative real-time PCR to examine their regulation at the transcriptional level, western blot analysis was used to validate the changes of some selected proteins on translational level. To our knowledge, this study is the first to analyze the proteomic changes in duck ovarian follicles following DTMUV infection. The protein-related information obtained in this study may be useful to understand the host response to DTMUV infection and the inherent mechanism of DTMUV replication and pathogenicity.
has issue date	2016-03-31(xsd:dateTime)
bibo:doi	10.3389/fmicb.2016.00463
bibo:pmid	27066001
has license	cc-by
sha1sum (hex)	3f1a7bb419c2658dfd71663cc87efd03a38f1716
schema:url	https://doi.org/10.3389/fmicb.2016.00463
resource representing a document's title	Quantitative Proteomic Analysis of Duck Ovarian Follicles Infected with Duck Tembusu Virus by Label-Free LC-MS
has PubMed Central identifier	PMC4815560
has PubMed identifier	27066001
schema:publication	Front Microbiol
resource representing a document's body	covid:3f1a7bb419c2658dfd71663cc87efd03a38f1716#body_text
is schema:about of	named entity 'regulation' named entity 'expression' named entity 'protein' named entity 'regulation' named entity 'virus' named entity 'proteins' named entity 'losses' named entity 'validate' named entity 'LC-MS' named entity 'COMPARATIVE' named entity 'LC-MS' covid:arg/3f1a7bb419c2658dfd71663cc87efd03a38f1716 named entity 'NANOFLOW HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY' named entity 'WESTERN BLOT ANALYSIS' named entity 'THEIR' named entity 'ANALYZED' named entity 'CHANGES' named entity 'SIGNIFICANT' named entity 'USING' named entity 'MECHANISM' named entity 'IS A' named entity 'RELATED' named entity 'ESSENTIAL' named entity 'QUANTITATIVE REAL-TIME PCR' named entity 'EXPRESSED' named entity 'LEVEL' named entity 'INVOLVED' named entity 'DUCK' named entity 'INDUSTRY' named entity 'PROTEIN EXPRESSION' named entity 'TEMBUSU VIRUS' named entity '2 WEEKS' named entity 'OVARIAN FOLLICLES' named entity 'CITATION' named entity 'QUANTITATIVE' named entity 'PROTEOMIC ANALYSIS' named entity 'INFECTED' named entity 'REPLICATION' named entity 'DECREASES' named entity 'REGULATED' named entity 'DUCKS' named entity 'DOWN REGULATED' named entity '1-2' named entity 'REAL-TIME PCR ' named entity 'TRANSCRIPTIONAL' named entity 'USED' named entity 'RNA PROCESSING' named entity 'REVEALED' named entity 'CAUSED' named entity 'IDENTIFIED' named entity 'CHINA' named entity 'MASSIVE' named entity 'INFECTION' named entity 'MAY BE' named entity 'PROCESSES' named entity 'CELLULAR' named entity 'PROTEOMIC' named entity 'PATHOGENIC' named entity 'TO EXAMINE' named entity 'QUANTITATIVE' named entity 'TRANSLATIONAL' named entity 'MODIFICATION' named entity 'PROTEOMIC ANALYSIS' named entity 'PROTEIN ' named entity 'PRESENT' named entity 'FOLLOWING' named entity 'NEWLY' named entity 'POST' named entity 'OBTAINED' named entity 'STUDY'

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