About: Early events of SARS coronavirus infection in vero cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Early events of SARS coronavirus infection in vero cells
Creator	Ling, A Ng, M Ooi, E Tan, S See, E
Source	Medline; PMC
abstract	An isolate from a patient in the recent severe acute respiratory syndrome (SARS) outbreak in Singapore was used to infect Vero E6 cells. This study concentrated on the first 30 min of infection. It was discovered that the SARS coronavirus attached, entered, and uncoated the nucleocapsids, all within a 30‐min period. At 5 min after infection, several virus particles lined the Vero cell plasma membrane. Virus particles were at various stages of fusion at the cell surface, since entry was not a synchronised process. After entry (10 and 15 min), spherical core particles moved into the cytoplasm within large vacuoles. Quite surprising at such early stages of infection (20 min), a virus‐induced change in the infected cells was evident. The induction of myelin‐like membrane whorls was obvious within the same vacuoles as the core particles. The significance of this virus‐induced change is unknown at this stage. By 25–30 min postinfection (p.i.), the spherical core particles appeared to be disassociating and, in their place, doughnut‐shaped electron‐dense structures were observed. These could be the virus genomes together with the helical nucleocapsids. They were no longer in large vacuoles but packaged into smaller vacuoles in the cytoplasm, and occasionally in small groups. J. Med. Virol. 71:323–331, 2003. © 2003 Wiley‐Liss, Inc.
has issue date	2003-09-04(xsd:dateTime)
bibo:doi	10.1002/jmv.10499
bibo:pmid	12966536
has license	no-cc
sha1sum (hex)	3f269bc731f8483c89ca6dbb39753bcda23a3d33
schema:url	https://doi.org/10.1002/jmv.10499
resource representing a document's title	Early events of SARS coronavirus infection in vero cells
has PubMed Central identifier	PMC7166834
has PubMed identifier	12966536
schema:publication	J Med Virol
resource representing a document's body	covid:3f269bc731f8483c89ca6dbb39753bcda23a3d33#body_text
is schema:about of	named entity 'SARS Coronavirus' named entity 'Coronavirus Infection' named entity 'SARS coronavirus' named entity 'vacuoles' named entity 'virus' named entity 'infection' named entity 'genomes' named entity 'virus' named entity 'myelin' named entity 'virus' named entity 'vacuole' named entity 'osmium tetroxide' named entity 'helical' named entity 'cell lines' named entity 'SARS' named entity 'natural host' named entity 'virus' named entity 'Vero E6' named entity 'myelin' named entity 'MDCK' named entity 'vacuoles' named entity 'virus envelope' named entity 'severe acute respiratory syndrome' named entity 'SARS' named entity 'virus' named entity 'vacuoles' named entity 'vacuoles' named entity 'ultrastructural' named entity 'nucleocapsids' named entity 'plasma membrane' named entity 'virus' named entity 'virus' named entity 'virus' named entity 'vacuoles' named entity 'Environmental Health' named entity 'electron-dense' named entity 'nucleocapsids' named entity 'cytoplasm' named entity 'coronaviruses' named entity 'SARS coronavirus' named entity 'Vietnam' named entity 'phosphate-buffered saline' named entity 'Vero E6' named entity 'cell culture' named entity 'vesicles' named entity 'SARS' named entity 'negative staining' named entity 'plasma membrane' named entity 'glutaraldehyde' named entity 'viruses' named entity 'virus' named entity 'cytoplasm' named entity 'SARS coronavirus' named entity 'cytoplasm' named entity 'plasma membrane' named entity 'Electron microscopy' named entity 'electron-dense' named entity 'SARS coronavirus' named entity 'electron-dense' named entity 'virus' named entity 'ethanol' named entity 'infection' named entity 'inoculation' named entity 'cytoplasm' named entity 'virus' named entity 'virus' named entity 'Vero E6' named entity 'virus replication' named entity 'cytoplasm' named entity 'viscosity' named entity 'HeLa' named entity 'cell lines' named entity 'SARS' named entity 'plasma membrane' named entity 'virus' named entity 'infection' named entity 'National University of Singapore'

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