About: Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification
Creator	Paul, Moushumi Wang, De-Guo Brewster, Jeffrey Tomasula, Peggy
source	PMC
abstract	The technique of loop-mediated isothermal amplification (LAMP) utilizes four (or six) primers targeting six (or eight) regions within a fairly small segment of a genome for amplification, with concentration higher than that used in traditional PCR methods. The high concentrations of primers used leads to an increased likelihood of non-specific amplification induced by primer dimers. In this study, a set of LAMP primers were designed targeting the prfA gene sequence of Listeria monocytogenes, and dimethyl sulfoxide (DMSO) as well as Touchdown LAMP were employed to increase the sensitivity and specificity of the LAMP reactions. The results indicate that the detection limit of this novel LAMP assay with the newly designed primers and additives was 10 fg per reaction, which is ten-fold more sensitive than a commercial Isothermal Amplification Kit and hundred-fold more sensitive than previously reported LAMP assays. This highly sensitive LAMP assay has been shown to detect 11 strains of Listeria monocytogenes, and does not detect other Listeria species (including Listeria innocua and Listeria invanovii), providing some advantages in specificity over commercial Isothermal Amplification Kits and previously reported LAMP assay.
has issue date	2015-04-07(xsd:dateTime)
bibo:doi	10.3390/molecules20046048
bibo:pmid	25853320
has license	cc-by
sha1sum (hex)	3fae11ec9abada619c2f2ea754eba8d43e9924d9
schema:url	https://doi.org/10.3390/molecules20046048
resource representing a document's title	Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification
has PubMed Central identifier	PMC6272222
has PubMed identifier	25853320
schema:publication	Molecules
resource representing a document's body	covid:3fae11ec9abada619c2f2ea754eba8d43e9924d9#body_text
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