About: Human matriptase/ST 14 proteolytically cleaves H7N9 hemagglutinin and facilitates the activation of influenza A/Shanghai/2/2013 virus in cell culture

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About: Human matriptase/ST 14 proteolytically cleaves H7N9 hemagglutinin and facilitates the activation of influenza A/Shanghai/2/2013 virus in cell culture Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Human matriptase/ST 14 proteolytically cleaves H7N9 hemagglutinin and facilitates the activation of influenza A/Shanghai/2/2013 virus in cell culture
Creator	Whittaker, Gary Straus, Marco
Source	Medline; PMC
abstract	BACKGROUND: Influenza is a zoonotic disease that infects millions of people each year resulting in hundreds of thousands of deaths, and in turn devastating pandemics. Influenza is caused by influenza viruses, including influenza A virus (IAV). There are many subtypes of IAV but only a few seem to be able to adapt to humans and to cause disease. In 2013, an H7N9 IAV subtype emerged in China that does not cause clinical symptoms in its chicken host but leads to severe infections when transmitted into humans. Since 2013, there have been six epidemic waves of H7N9 with 1567 laboratory‐confirmed human infections and 615 deaths. Pathogenicity of IAV is complex, but a crucial feature contributing to virulence is the activation of the hemagglutinin (HA) fusion protein by host proteases that triggers membrane fusion and leads to subsequent virus propagation. METHODS: 293T, VERO, and MDCK cells were used to conduct Western blot analysis, immunofluorescence assays, and pseudoparticle and live virus infections, and to evaluate H7N9 HA cleavage‐activation. RESULTS/CONCLUSIONS: We show that human matriptase/ST 14 is able to cleave H7N9 HA. Cleavage of H7N9 HA expressed in cell culture results in fusogenic HA and syncytia formation. In infection studies with viral pseudoparticles carrying matriptase/ST 14‐activated H7N9 HA, we observed a high infectivity of cells. Finally, human matriptase/ST 14 also activated H7N9 live virus which resulted in high infectivity. Our data demonstrate that human matriptase/ST 14 is a likely candidate protease to promote H7N9 infections in humans.
has issue date	2019-12-09(xsd:dateTime)
bibo:doi	10.1111/irv.12707
bibo:pmid	31820577
has license	cc-by
sha1sum (hex)	3fd4c59ae94e2ecc44c0c40d678dd18e31ddb7c7
schema:url	https://doi.org/10.1111/irv.12707
resource representing a document's title	Human matriptase/ST 14 proteolytically cleaves H7N9 hemagglutinin and facilitates the activation of influenza A/Shanghai/2/2013 virus in cell culture
has PubMed Central identifier	PMC7040964
has PubMed identifier	31820577
schema:publication	Influenza Other Respir Viruses
resource representing a document's body	covid:3fd4c59ae94e2ecc44c0c40d678dd18e31ddb7c7#body_text
is schema:about of	named entity 'H7N9' named entity 'activation' named entity '2013' named entity 'MATRIPTASE' named entity 'ACTIVATION' named entity 'INFLUENZA A' named entity 'TURN' named entity 'IS A' named entity 'HEMAGGLUTININ' named entity 'CELL CULTURE' named entity 'YEAR' named entity 'HUMAN' named entity 'VIRUS' named entity 'CLEAVES' named entity 'INFLUENZA' named entity 'PEOPLE' named entity 'INFECTS' named entity 'BACKGROUND' named entity 'DEATHS' named entity 'ZOONOTIC DISEASE' named entity 'PANDEMICS' named entity 'RESULTING IN' covid:arg/3fd4c59ae94e2ecc44c0c40d678dd18e31ddb7c7 named entity 'pandemics' named entity 'turn' named entity 'hemagglutinin' named entity 'virus' named entity 'matriptase' named entity 'Virus' named entity 'fusion protein' named entity 'virions' named entity 'H7N9' named entity 'murine leukemia virus' named entity 'virus' named entity 'H7N9' named entity 'influenza' named entity 'serine proteases' named entity 'poultry' named entity 'H7N9' named entity 'gastro-intestinal tract' named entity 'poultry' named entity 'TMPRSS2' named entity 'viruses' named entity 'H1N1' named entity 'negative control' named entity 'TMPRSS2' named entity 'trypsin' named entity 'trypsin' named entity 'transfection' named entity 'antibody' named entity 'disease symptoms' named entity 'H7N9' named entity 'epithelial cells' named entity 'recombinant' named entity 'respiratory tract' named entity 'viruses' named entity 'poultry' named entity 'Swine flu' named entity 'C-terminus' named entity 'matriptase' named entity 'trypsin' named entity 'Influenza A viruses' named entity 'avian influenza' named entity 'positive control' named entity 'matriptase' named entity 'Trypsin' named entity 'recombinant' named entity 'fusogenic' named entity 'cell lysis' named entity 'host cells' named entity 'Western blot analysis' named entity 'culling' named entity 'H2N2' named entity 'influenza' named entity 'VSV-G' named entity 'luciferase' named entity 'H7N9' named entity 'infectivity' named entity 'H7N9' named entity 'H7N9'

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