About: Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids
Creator	Snijder, Joost Veesler, David Walls, Alexandra Burrell, Anika Dosey, Annie Borst, Andrew Kollman, Justin Reddy, Vijay
Source	Elsevier; Medline; PMC
abstract	Single particle cryo-electron microscopy (cryoEM) is becoming widely adopted as a tool for structural characterization of biomolecules at near-atomic resolution. Vitrification of the sample to obtain a dense distribution of particles within a single field of view remains a major bottleneck for the success of such experiments. Here, we describe a simple and cost-effective method to increase the density of frozen-hydrated particles on grids with holey carbon support films. It relies on performing multiple rounds of sample application and blotting prior to plunge freezing in liquid ethane. We show that this approach is generally applicable and significantly increases particle density for a range of samples, such as small protein complexes, viruses and filamentous assemblies. The method is versatile, easy to implement, minimizes sample requirements and can enable characterization of samples that would otherwise resist structural studies using single particle cryoEM.
has issue date	2017-04-01(xsd:dateTime)
bibo:doi	10.1016/j.jsb.2017.02.008
bibo:pmid	28254381
has license	green-oa
sha1sum (hex)	40377a40529111e36aec0a32f3d857c008379391
schema:url	https://doi.org/10.1016/j.jsb.2017.02.008
resource representing a document's title	Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids
has PubMed Central identifier	PMC5400742
has PubMed identifier	28254381
schema:publication	Journal of Structural Biology
resource representing a document's body	covid:40377a40529111e36aec0a32f3d857c008379391#body_text
is schema:about of	named entity 'ACCESS' named entity 'PUBLIC' named entity 'application' named entity 'grids' named entity 'cryo-electron microscopy' named entity 'HHS' named entity 'CRYO-ELECTRON MICROSCOPY' named entity 'ROUNDS' named entity 'EFFECTIVE' named entity 'VIRUSES' named entity 'EASY' named entity 'FIELD OF VIEW' named entity 'NEAR-ATOMIC' named entity 'IMPROVES' named entity 'SAMPLE' named entity 'SIMPLE' named entity 'DISTRIBUTION' named entity 'PRIOR TO' named entity 'APPROACH' named entity 'PARTICLES' named entity 'ROUNDS' named entity 'PROTEIN COMPLEXES' named entity 'SAMPLE' named entity 'PLUNGE FREEZING' named entity 'CRYOEM' named entity 'SAMPLES' named entity 'SMALL' named entity 'RESOLUTION' named entity 'APPLICATION' named entity 'USING' named entity 'BLOTTING' named entity 'INCREASES' named entity 'STRUCTURAL' named entity 'TOOL' named entity 'FILMS' named entity 'CHARACTERIZATION' named entity 'A MAJOR' named entity 'VITRIFICATION' named entity 'PARTICLE' named entity 'DENSITY' named entity 'DESCRIBE' named entity 'TO INCREASE' named entity 'DENSE' named entity 'BIOMOLECULES' named entity 'CARBON' named entity 'VITRIFICATION' named entity 'EXPERIMENTS' named entity 'BLOTTING' named entity 'MULTIPLE' named entity 'APPLICATION' named entity 'HHS' named entity 'METHOD' named entity 'OBTAIN' named entity 'SUPPORT' named entity 'COST' named entity 'FILAMENTOUS' named entity 'PERFORMING' named entity 'DENSITY' named entity 'PARTICLE' named entity 'LIQUID' named entity 'RANGE' named entity 'APPLICABLE' named entity 'FROZEN' named entity 'ETHANE' named entity 'STRUCTURAL CHARACTERIZATION' named entity 'ENABLE' named entity 'REQUIREMENTS' named entity 'GENERALLY' named entity 'ADOPTED' named entity 'MULTIPLE' named entity 'HERE' named entity 'SINGLE PARTICLE CRYO-ELECTRON MICROSCOPY' named entity 'STUDIES' named entity 'SUCCESS'

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