About: Peste des Petits Ruminants Virus Enters Caprine Endometrial Epithelial Cells via the Caveolae-Mediated Endocytosis Pathway

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About: Peste des Petits Ruminants Virus Enters Caprine Endometrial Epithelial Cells via the Caveolae-Mediated Endocytosis Pathway Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Peste des Petits Ruminants Virus Enters Caprine Endometrial Epithelial Cells via the Caveolae-Mediated Endocytosis Pathway
Creator	Yang, Bo Wang, Chen Chen, Shuying Chen, Zhijie Guo, Hui Jia, Peilong Qi, Xuefeng Wang, Jingyu Wang, Ting Xue, Qinghong Cosby, Sara Navarro-Garcia, Fernando
Source	PMC
abstract	Peste des petits ruminants virus (PPRV) causes an acute and highly contagious disease of sheep and goats and has spread with alarming speed around the world. The pathology of Peste des petits ruminants is linked to retrogressive changes and necrotic lesions in lymphoid tissues and epithelial cells. However, the process of PPRV entry into host epithelial cells remains largely unknown. Here, we performed a comprehensive study of the entry mechanism of PPRV into caprine endometrial epithelial cells (EECs). We clearly demonstrated that PPRV internalization was inhibited by chloroquine and ammonium chloride, which elevate the pH of various organelles. However, PPRV entry was not affected by chlorpromazine and knockdown of the clathrin heavy chain in EECs. In addition, we found that the internalization of PPRV was dependent on dynamin and membrane cholesterol and was suppressed by silencing of caveolin-1. Macropinocytosis did not play a role, but phosphatidylinositol 3-kinase (PI3K) was required for PPRV internalization. Cell type and receptor-dependent differences indicated that PPRV entry into caprine fetal fibroblast cells (FFCs) occurred via a different route. Taken together, our findings demonstrate that PPRV enters EECs through a cholesterol-dependent caveolae-mediated uptake mechanism that is pH-dependent and requires dynamin and PI3K but is independent of clathrin. This potentially provides insight into the entry mechanisms of other morbilliviruses.
has issue date	2018-02-15(xsd:dateTime)
bibo:doi	10.3389/fmicb.2018.00210
bibo:pmid	29497407
has license	cc-by
sha1sum (hex)	4091e78b118eed89910b5089f4303a51d7e4c4fa
schema:url	https://doi.org/10.3389/fmicb.2018.00210
resource representing a document's title	Peste des Petits Ruminants Virus Enters Caprine Endometrial Epithelial Cells via the Caveolae-Mediated Endocytosis Pathway
has PubMed Central identifier	PMC5818419
has PubMed identifier	29497407
schema:publication	Front Microbiol
resource representing a document's body	covid:4091e78b118eed89910b5089f4303a51d7e4c4fa#body_text
is schema:about of	named entity 'ENDOMETRIAL' named entity 'demonstrate' named entity 'caprine' named entity 'cholesterol' named entity 'inhibited' named entity 'PI3K' named entity 'route' named entity 'dynamin' named entity 'This' covid:arg/4091e78b118eed89910b5089f4303a51d7e4c4fa named entity 'FOUND' named entity 'HIGHLY CONTAGIOUS DISEASE' named entity 'PLAY' named entity 'INTERNALIZATION' named entity 'COMPREHENSIVE' named entity 'REQUIRED' named entity 'WORLD' named entity 'ENTRY INTO HOST' named entity 'EPITHELIAL CELLS' named entity 'ROUTE' named entity 'DID' named entity 'STUDY' named entity 'GOATS' named entity 'LESIONS' named entity 'TAKEN' named entity 'PHOSPHATIDYLINOSITOL 3-KINASE' named entity 'OUR' named entity 'BUT' named entity 'MORBILLIVIRUSES' named entity 'SILENCING' named entity 'LYMPHOID TISSUES' named entity 'MACROPINOCYTOSIS' named entity 'PROCESS' named entity 'DEPENDENT' named entity 'HERE' named entity 'FETAL FIBROBLAST' named entity 'ALARMING' named entity 'ENDOMETRIAL' named entity 'NECROTIC' named entity 'SPEED' named entity 'MECHANISMS' named entity 'ENTRY' named entity 'ORGANELLES' named entity 'ACUTE' named entity 'MEDIATED' named entity 'SPREAD' named entity 'EPITHELIAL CELLS' named entity 'MEMBRANE' named entity 'PROVIDES' named entity 'PESTE DES PETITS RUMINANTS' named entity 'ADDITION' named entity 'INDEPENDENT OF' named entity 'SHEEP' named entity 'PESTE DES PETITS RUMINANTS VIRUS' named entity 'CAVEOLAE-MEDIATED ENDOCYTOSIS PATHWAY' named entity 'CAPRINE' named entity 'CITATION' named entity 'CLATHRIN' named entity 'OCCURRED' named entity 'PATHOLOGY' named entity 'CLATHRIN HEAVY CHAIN' named entity 'CELL TYPE' named entity 'AFFECTED' named entity 'MECHANISM' named entity 'CHLORPROMAZINE' named entity 'UNKNOWN' named entity 'ELEVATE' named entity 'VARIOUS' named entity 'PH-' named entity 'CAVEOLAE'

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