About: Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus

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About: Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus
Creator	Sztuba-Solińska, Joanna Bujarski, Jozef Fanning, Sean Horn, James
Source	Elsevier; Medline; PMC
abstract	Abstract We have previously described the efficient homologous recombination system between 5′ subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-acting RNA motifs, the Bbox region (in the intercistronic RNA3 sequence) and the RNA3 packaging element (PE, in the movement protein ORF), reduced crossover frequencies in protoplasts. Additionally, the modification of Bbox-like element in the 5′ UTR region strongly debilitated crossovers. Along the lines of these observations, RNA3 mutants not expressing CP or expressing mutated CPs also reduced recombination. A series of reciprocal transfections demonstrated a functional crosstalk between the Bbox and PE elements. Altogether, our data imply the role of CP in sgRNA3a-directed recombination by either facilitating the interaction of the RNA substrates and/or by creating roadblocks for the viral replicase.
has issue date	2012-12-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2012.10.001
bibo:pmid	23079110
has license	els-covid
sha1sum (hex)	40bb91258ab5515b39230e66bdf4f556ab10a17c
schema:url	https://doi.org/10.1016/j.virusres.2012.10.001
resource representing a document's title	Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus
has PubMed Central identifier	PMC7114393
has PubMed identifier	23079110
schema:publication	Virus Research
resource representing a document's body	covid:40bb91258ab5515b39230e66bdf4f556ab10a17c#body_text
is schema:about of	named entity 'protoplasts' named entity 'reciprocal' named entity 'expressing' named entity 'barley' named entity 'viral' named entity 'subgenomic' named entity 'interaction' named entity 'Mutations' named entity 'COAT PROTEIN' named entity 'RNA RECOMBINATION' named entity 'UTR REGION' named entity 'BARLEY' named entity 'CIS' named entity 'PROTEIN ORF' named entity 'SYSTEM' named entity 'MOVEMENT PROTEIN' named entity 'ROLE' named entity 'SOLI' named entity 'OUR' named entity 'MUTATED' named entity 'REPLICASE' named entity 'RECIPROCAL' named entity 'REDUCED' named entity 'HAVE' named entity 'RECOMBINATION' named entity 'FUNCTIONAL' named entity 'RNA3' named entity 'LIKE' named entity 'PREVIOUSLY' named entity 'BINDING' named entity 'BROME MOSAIC VIRUS' named entity 'DEMONSTRATED' named entity 'CROSSOVER' named entity 'GENOMIC' named entity 'PROTEIN ' named entity 'TRANSFECTIONS' named entity 'BINDING' named entity 'MUTATIONS' named entity 'RNA' named entity 'MOTIFS' named entity 'MODIFICATION' named entity 'INTERACTION' named entity 'OBSERVATIONS' named entity 'HERE' named entity 'DIRECTED' named entity 'CREATING' named entity 'EITHER' named entity 'PACKAGING' named entity 'SERIES' named entity 'CIS' named entity 'BROME MOSAIC VIRUS' named entity 'SEQUENCE' named entity 'REGION' named entity 'EFFICIENT' named entity 'ELEMENTS' named entity 'VIRAL' named entity 'LINES' named entity 'CROSSTALK' named entity 'BBOX' named entity 'HOMOLOGOUS RECOMBINATION' named entity 'ALTERATIONS' named entity 'CPS' named entity 'DEBILITATED' named entity 'MOTIFS' named entity 'MUTANTS' named entity 'PROTOPLASTS' named entity 'FREQUENCIES' named entity 'THESE' named entity 'FACILITATING' named entity 'DATA' named entity 'DESCRIBED' named entity 'COAT PROTEIN' named entity 'ELEMENT' named entity 'RNA' named entity 'reduced' named entity 'data' named entity 'sequence' named entity 'binding'

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