About: A Co-Opted DEAD-Box RNA Helicase Enhances Tombusvirus Plus-Strand Synthesis

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Co-Opted DEAD-Box RNA Helicase Enhances Tombusvirus Plus-Strand Synthesis
Creator	Nagy, P Pogany, J Kovalev, N
Source	PMC
abstract	Replication of plus-strand RNA viruses depends on recruited host factors that aid several critical steps during replication. In this paper, we show that an essential translation factor, Ded1p DEAD-box RNA helicase of yeast, directly affects replication of Tomato bushy stunt virus (TBSV). To separate the role of Ded1p in viral protein translation from its putative replication function, we utilized a cell-free TBSV replication assay and recombinant Ded1p. The in vitro data show that Ded1p plays a role in enhancing plus-strand synthesis by the viral replicase. We also find that Ded1p is a component of the tombusvirus replicase complex and Ded1p binds to the 3′-end of the viral minus-stranded RNA. The data obtained with wt and ATPase deficient Ded1p mutants support the model that Ded1p unwinds local structures at the 3′-end of the TBSV (−)RNA, rendering the RNA compatible for initiation of (+)-strand synthesis. Interestingly, we find that Ded1p and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which is another host factor for TBSV, play non-overlapping functions to enhance (+)-strand synthesis. Altogether, the two host factors enhance TBSV replication synergistically by interacting with the viral (−)RNA and the replication proteins. In addition, we have developed an in vitro assay for Flock house virus (FHV), a small RNA virus of insects, that also demonstrated positive effect on FHV replicase activity by the added Ded1p helicase. Thus, two small RNA viruses, which do not code for their own helicases, seems to recruit a host RNA helicase to aid their replication in infected cells.
has issue date	2012-02-16(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1002537
bibo:pmid	22359508
has license	cc-by
sha1sum (hex)	412833ef524797198177f582425f82e45c0f7a06
schema:url	https://doi.org/10.1371/journal.ppat.1002537
resource representing a document's title	A Co-Opted DEAD-Box RNA Helicase Enhances Tombusvirus Plus-Strand Synthesis
has PubMed Central identifier	PMC3280988
has PubMed identifier	22359508
schema:publication	PLoS Pathog
resource representing a document's body	covid:412833ef524797198177f582425f82e45c0f7a06#body_text
is schema:about of	named entity 'host' named entity 'host' named entity 'cells' named entity 'data' named entity 'helicase' covid:arg/412833ef524797198177f582425f82e45c0f7a06 named entity 'helicases' named entity 'protein translation' named entity 'developed' named entity 'yeast' named entity 'depends' named entity 'RNA' named entity 'host factor' named entity 'local' named entity 'structures' named entity 'recombinant' named entity 'strand' named entity 'binds' named entity 'insects' named entity 'replicase' named entity 'replication' named entity 'data' named entity 'Flock house virus' named entity 'tombusvirus' named entity 'RNA' named entity 'small RNA' named entity 'RNA helicase' named entity 'RNA' named entity 'replicase' named entity 'helicases' named entity 'RNA virus' named entity 'Tombusvirus' named entity 'RNA' named entity 'RdRp' named entity 'replicase' named entity 'yeast' named entity 'RdRp' named entity 'RNA viruses' named entity 'KOH' named entity 'TBSV' named entity 'genome' named entity 'ATPase' named entity 'proteomics' named entity 'RNA' named entity 'yeast' named entity 'TBSV' named entity 'replicase' named entity 'Protein' named entity 'GAPDH' named entity 'mRNA' named entity 'tTA' named entity 'replicase' named entity 'PCR' named entity 'FHV' named entity 'enhancer sequence' named entity 'TBSV' named entity 'TBSV' named entity 'Hsp70' named entity 'replicase' named entity 'mRNA' named entity 'replicase' named entity 'replicase' named entity 'TBSV' named entity 'similar function' named entity 'TBSV' named entity 'Protein expression' named entity 'yeast' named entity 'GAPDH' named entity 'yeast' named entity 'helicase' named entity 'FHV' named entity 'RNA' named entity 'yeast' named entity 'viral RNA' named entity 'tombusvirus' named entity 'replicase' named entity 'yeast' named entity 'PCR' named entity 'S4D'

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