About: Two Amino Acid Changes at the N-Terminus of Transmissible Gastroenteritis Coronavirus Spike Protein Result in the Loss of Enteric Tropism

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Two Amino Acid Changes at the N-Terminus of Transmissible Gastroenteritis Coronavirus Spike Protein Result in the Loss of Enteric Tropism Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Two Amino Acid Changes at the N-Terminus of Transmissible Gastroenteritis Coronavirus Spike Protein Result in the Loss of Enteric Tropism
Creator	Enjuanes, L Ballesteros, M Nchez, C
source	Elsevier; Medline; PMC
abstract	Abstract To study the molecular basis of TGEV tropism, a collection of recombinants between the PUR46-MAD strain of transmissible gastroenteritis coronavirus (TGEV) infecting the enteric and respiratory tracts and the PTV strain, which only infects the respiratory tract, was generated. The recombinant isolation frequency was about 10−9recombinants per nucleotide and was 3.7-fold higher at the 5′-end of the S gene than in other areas of the genome. Thirty recombinants were plaque purified and characterized phenotypically and genetically. All recombinant viruses had a single crossover and had inherited the 5′- and 3′-halves of their genome from the enteric and respiratory parents, respectively. Recombinant viruses were classified into three groups, named 1 to 3, according to the location of the crossover. Group 1 recombinants had the crossover in the S gene, while in Groups 2 and 3 the crossovers were located in ORF1b and ORF1a, respectively. The tropism of the recombinants was studied. Recombinants of Group 1 had enteric and respiratory tropism, while Group 2 recombinants infected the respiratory, but not the enteric, tract. Viruses of both groups differed by two nucleotide changes at positions 214 and 655. Both changes may be in principle responsible for the loss of enteric tropism but only the change in nucleotide 655 was specifically found in the respiratory isolates and most likely this single nucleotide change, which leads to a substitution in amino acid 219 of the S protein, was responsible for the loss of enteric tropism in the closely related PUR-46 isolates. The available data indicate that in order to infect enteric tract cells with TGEV, two different domains of the S protein, mapping between amino acids 522 and 744 and around amino acid 219, respectively, are involved. The first domain binds to porcine aminopeptidase N, the cellular receptor for TGEV. In the other domain maps a second factor of undefined nature but which may be the binding site for a coreceptor essential for the enteric tropism of TGEV.
has issue date	1997-01-20(xsd:dateTime)
bibo:doi	10.1006/viro.1996.8344
bibo:pmid	9018137
has license	els-covid
sha1sum (hex)	4235f9c6e84312765672dd1b422ce68772ff2408
schema:url	https://doi.org/10.1006/viro.1996.8344
resource representing a document's title	Two Amino Acid Changes at the N-Terminus of Transmissible Gastroenteritis Coronavirus Spike Protein Result in the Loss of Enteric Tropism
has PubMed Central identifier	PMC7130969
has PubMed identifier	9018137
schema:publication	Virology
resource representing a document's body	covid:4235f9c6e84312765672dd1b422ce68772ff2408#body_text
is schema:about of	named entity 'areas' named entity 'loss' named entity 'molecular' named entity 'tropism' named entity 'Amino Acid' covid:arg/4235f9c6e84312765672dd1b422ce68772ff2408 named entity 'strain' named entity 'frequency' named entity 'involved' named entity 'respiratory tract' named entity 'responsible' named entity 'inherited' named entity 'nucleotide' named entity 'data' named entity 'substitution' named entity 'Recombinant' named entity 'enteric' named entity 'binding site' named entity 'nucleotide' named entity 'gene' named entity 'recombinant' named entity 'recombinants' named entity 'nucleotide change' named entity 'viruses' named entity 'molecular basis' named entity 'Academic Press' named entity 'N-Terminus' named entity 'PUR' named entity 'tropism' named entity 'recombinants' named entity 'virus strain' named entity 'sequence analysis' named entity 'virus' named entity 'single nucleotide' named entity 'MAbs' named entity 'porcine' named entity 'gene' named entity 'MAbs' named entity 'TGEV' named entity 'protein' named entity 'viruses' named entity 'tyrosine' named entity 'Coronaviruses' named entity 'C11' named entity 'recombinants' named entity 'nucleotide' named entity 'PRCVs' named entity 'tropism' named entity 'Pharmacia' named entity 'tropism' named entity 'single point mutation' named entity 'virus' named entity 'RNA' named entity 'Callebaut' named entity 'protein' named entity 'viruses' named entity '2.0' named entity 'amino acid' named entity 'virus' named entity 'TGEV' named entity 'TGEV' named entity 'virus strain' named entity 'protein' named entity 'gene' named entity 'recombinant' named entity 'MAbs' named entity 'viruses' named entity 'TGEV' named entity 'N-terminal' named entity 'subcloned' named entity 'viruses' named entity 'virus' named entity 'recombinants' named entity 'Nucleotides' named entity 'recombinants' named entity 'nucleotides' named entity 'recombinants' named entity 'virus' named entity 'virus'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software