About: The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella zoster virus, adenovirus and Chlamydia trachomatis from eye swabs

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About: The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella zoster virus, adenovirus and Chlamydia trachomatis from eye swabs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella zoster virus, adenovirus and Chlamydia trachomatis from eye swabs
Creator	Bennett, Susan Gunson, Rory Carman, William
Source	Elsevier; Medline; PMC
abstract	Abstract Infectious conjunctivitis can be difficult to distinguish clinically due to the considerable overlap in clinical presentation so clinical diagnosis of conjunctivitis is often insufficient. It is therefore necessary to have a rapid diagnostic test that differentiates between the different causes of infectious conjunctivitis. Screening clinical samples by sample type/syndrome based multiplex real time PCR would allow for rapid detection of a variety of pathogens simultaneously, which will in turn aid in the treatment and clinical management of the patient. A multiplex real-time PCR assay for rapid and simultaneous detection of HSV 1 and 2, VZV, adenovirus and Chlamydia trachomatis (C. trachomatis) from eye swabs was developed and evaluated. The multiplex assay was shown to be sensitive, specific and robust. Reductions in sample turn around times have been achieved by reducing the amount of separate tests needed to be carried out.
has issue date	2013-04-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2012.08.020
bibo:pmid	23000752
has license	els-covid
sha1sum (hex)	42ce4e5eac6e69f5dc036aa8a7998daef1d9b0cf
schema:url	https://doi.org/10.1016/j.jviromet.2012.08.020
resource representing a document's title	The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella zoster virus, adenovirus and Chlamydia trachomatis from eye swabs
has PubMed Central identifier	PMC7172154
has PubMed identifier	23000752
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:42ce4e5eac6e69f5dc036aa8a7998daef1d9b0cf#body_text
is schema:about of	named entity 'adenovirus' named entity 'real-time PCR' named entity 'multiplex' named entity 'simplex' named entity 'CONSIDERABLE' named entity 'CLINICAL PRESENTATION' named entity 'HAVE' named entity 'REAL TIME PCR' named entity 'DIFFICULT' named entity 'multiplex' named entity 'conjunctivitis' named entity 'clinical samples' named entity 'Chlamydia trachomatis' named entity 'adenovirus' named entity 'keratoconjunctivitis' named entity 'Maidenhead' named entity 'clinical presentation' named entity 'conjunctivitis' named entity 'real-time PCR' named entity 'pathogens' named entity 'Cheshire' named entity 'conjunctivitis' named entity 'VZV' named entity 'sensitive and specific' named entity 'PCR' named entity 'clinical samples' named entity 'VZV' named entity 'primers' named entity 'Applied Biosystems' named entity 'detection limit' named entity 'C. trachomatis' named entity 'positive control' named entity 'multiplex assay' named entity 'C. trachomatis' named entity 'parasites' named entity 'genitourinary medicine' named entity 'C. trachomatis' named entity 'C. trachomatis' named entity 'multiplex assay' named entity 'clinical diagnosis' named entity 'Belgium' named entity 'Swedish' named entity 'Seraing' named entity 'Primer' named entity 'PCR' named entity 'viruses' named entity 'multiplex PCR' named entity 'PCR' named entity 'positive control' named entity 'Crawley' named entity 'nucleic acid amplification tests' named entity 'gonorrhoea' named entity 'infection' named entity 'multiplex assay' named entity 'adenovirus' named entity 'bacteria' named entity 'HSV 1' named entity 'reproducibility' named entity 'western world' named entity 'dye' named entity 'Qiagen' named entity 'fluorescence' named entity 'adenovirus' named entity 'conjunctivitis' named entity 'C. trachomatis' named entity 'conjunctivitis' named entity 'positive controls' named entity '5 in 1' named entity 'primers' named entity 'PCR' named entity 'C. trachomatis' named entity 'infection' named entity 'real-time PCR' named entity 'pathogens' named entity 'PCR' named entity 'VZV' named entity 'real time PCR' named entity 'sensitive and specific' named entity 'C. trachomatis'

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