About: Molecular characterization of Cryptosporidium isolates from diarrheal dairy calves in France

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Molecular characterization of Cryptosporidium isolates from diarrheal dairy calves in France
Creator	Follet, Jérôme Polack, Bruno Chenafi, Ilham Chevillot, Aurélie Julien, Christine Mammeri, Mohamed Thomas, Myriam Vallée, Isabelle Adjou, Karim
source	Elsevier; Medline; PMC
abstract	Cryptosporidium is an obligate intracellular protist parasite infecting a wide range of vertebrate hosts and causes significant intestinal disease in both animals and humans, as some species are zoonotic. Cattle and especially calves have been identified as one of the most common reservoirs of this protist. However, little is known about the genetics of Cryptosporidium in calves in some regions of France. The aim of this study was to detect and isolate Cryptosporidium spp. in faecal samples from naturally infected pre-weaned calves (≤45 days-old) in France. A total of 35 diarrhoeic pre-weaned calf faecal samples were collected from 26 dairy cattle farms in six departments (French administrative provinces). Cryptosporidium presence was established by microscopically screening samples for oocystes with an immunofluorescent (DFA) staining method. DFA-positive samples were then analysed by PCR-RFLP and 18S rRNA gene sequencing to determine species. Cryptosporidium parvum-positive samples were subtyped via nested PCR analysis of a partial fragment of the 60 kDa glycoprotein (gp60) gene product. Data were then integrated into phylogenetic tree analysis. DFA revealed the presence of Cryptosporidium oocysts in 31 out of 35 (88%) samples. Combined with 18S rRNA gene analysis results, C. parvum was detected in 30 samples. Subtyping analysis in 27/30 samples (90%) of the C. parvum isolates revealed two zoonotic subtype families, IIa (24/27) and IId (3/27). Four subtypes were recognised within the subtype family IIa, including the hypertransmissible IIaA15G2R1 subtype that is the most frequently reported worldwide (21/27), IIaA17G3R1 (1/27), IIaA17G1R1 (1/27), and IIaA19G1R1 (1/27). Two subtypes were recognised within the IId subtype family including IIdA22G1 (2/27) and IIdA27G1 (1/27). These findings illustrate the high occurrence of Cryptosporidium in calves in dairy herds and increase the diversity of molecularly characterised C. parvum isolates with the first description of IIaA17G3R1, IIaA19G1R1, and IId subtypes in France. The presence of zoonotic C. parvum subtype families (IIa, IId) in this study suggests that pre-weaned calves are likely to be a significant reservoir of zoonotic C. parvum, and highlights the importance of animal to human cryptosporidiosis transmission risk. Further molecular studies in calves and small ruminants from other French regions are required to better understand the epidemiology of cryptosporidiosis in France.
has issue date	2019-07-24(xsd:dateTime)
bibo:doi	10.1016/j.vprsr.2019.100323
bibo:pmid	31796198
has license	no-cc
sha1sum (hex)	42ed4c4f257b2c9776eacccb1fc093132df5f94a
schema:url	https://doi.org/10.1016/j.vprsr.2019.100323
resource representing a document's title	Molecular characterization of Cryptosporidium isolates from diarrheal dairy calves in France
has PubMed Central identifier	PMC7103931
has PubMed identifier	31796198
schema:publication	Vet Parasitol Reg Stud Reports
resource representing a document's body	covid:42ed4c4f257b2c9776eacccb1fc093132df5f94a#body_text
is schema:about of	named entity 'DAIRY CALVES' named entity 'MOLECULAR' named entity 'screening' named entity 'French regions' named entity 'determine' named entity 'disease' named entity 'illustrate' named entity 'detect' named entity 'collected' named entity 'France' named entity 'PHYLOGENETIC TREE ANALYSIS' named entity 'SAMPLES' named entity 'KDA' named entity '18S RRNA GENE' named entity 'CALVES' named entity 'OLD' named entity 'DAYS' named entity 'SIGNIFICANT' named entity 'WORLDWIDE' named entity 'KNOWN' named entity 'REPORTED' named entity 'COMMON' named entity 'PCR ANALYSIS' named entity 'HAVE' named entity 'FARMS' named entity 'FREQUENTLY' named entity 'DETECTED' named entity 'ISOLATE' named entity 'HIGH' named entity 'PRESENCE OF' named entity 'WEANED' named entity 'LIKELY' named entity 'DFA' named entity 'STAINING METHOD' named entity 'PROTIST' named entity 'TOTAL' named entity 'ESTABLISHED' named entity 'ANALYSIS' named entity 'IIA' named entity 'STUDIES' named entity 'OOCYSTS' named entity 'GENE SEQUENCING' named entity 'ISOLATES' named entity 'REQUIRED' named entity 'REGIONS' named entity 'RESERVOIR' named entity 'ANIMAL' named entity 'EPIDEMIOLOGY' named entity 'INCLUDING' named entity 'CALF' named entity 'PARTIAL' named entity 'GENE ANALYSIS' named entity 'REVEALED' named entity 'INTESTINAL DISEASE' named entity 'FRAGMENT OF' named entity 'SCREENING' named entity 'IMMUNOFLUORESCENT' named entity 'DAIRY' named entity 'FRENCH' named entity 'DETERMINE' named entity 'TRANSMISSION' named entity 'COLLECTED' named entity 'GLYCOPROTEIN' named entity 'POSITIVE' named entity 'PARASITE' named entity 'AIM' named entity 'SPECIES' named entity '282' named entity 'STUDY' named entity 'CAUSES' named entity 'PRESENCE' named entity 'THESE' named entity 'HUMAN'

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