About: Divergent roles of β‐ and γ‐actin isoforms during spread of vaccinia virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Divergent roles of β‐ and γ‐actin isoforms during spread of vaccinia virus
Creator	Chaponnier, Christine Latham, Sharissa Lynn, Helena Newsome, Timothy Bishara Marzook, N Georges, \| Grau, E Mckenzie, \|
Source	Medline; PMC
abstract	Actin is a major component of the cytoskeleton and is present as two isoforms in non‐muscle cells: β‐ and γ‐cytoplasmic actin. These isoforms are strikingly conserved, differing by only four N‐terminal amino acids. During spread from infected cells, vaccinia virus (VACV) particles induce localized actin nucleation that propel virus to surrounding cells and facilitate cell‐to‐cell spread of infection. Here we show that virus‐tipped actin comets are composed of β‐ and γ‐actin. We employed isoform‐specific siRNA knockdown to examine the role of the two isoforms in VACV‐induced actin comets. Despite the high level of similarity between the actin isoforms, and their colocalization, VACV‐induced actin nucleation was dependent exclusively on β‐actin. Knockdown of β‐actin led to a reduction in the release of virus from infected cells, a phenotype dependent on virus‐induced Arp2/3 complex activity. We suggest that local concentrations of actin isoforms may regulate the activity of cellular actin nucleator complexes.
has issue date	2017-03-17(xsd:dateTime)
bibo:doi	10.1002/cm.21356
bibo:pmid	28218453
has license	no-cc
sha1sum (hex)	4341256921ce1b602b3a31db091950265fe573c4
schema:url	https://doi.org/10.1002/cm.21356
resource representing a document's title	Divergent roles of β‐ and γ‐actin isoforms during spread of vaccinia virus
has PubMed Central identifier	PMC7162416
has PubMed identifier	28218453
schema:publication	Cytoskeleton (Hoboken)
resource representing a document's body	covid:4341256921ce1b602b3a31db091950265fe573c4#body_text
is schema:about of	covid:arg/4341256921ce1b602b3a31db091950265fe573c4 named entity 'Actin' named entity 'actin' named entity 'Actin filaments' named entity 'isoforms' named entity 'primer' named entity 'DMEM' named entity 'actin' named entity 'arginine' named entity 'classical swine fever' named entity 'actin' named entity 'Virus' named entity 'actin' named entity 'nucleation' named entity 'GBM' named entity 'microtubule' named entity 'epithelial cells' named entity 'DMEM' named entity 'image processing' named entity 'fibroblasts' named entity 'photographic film' named entity 'A36' named entity 'one-way ANOVA' named entity 'nucleation' named entity 'ROCK' named entity 'nucleation' named entity 'metaphase' named entity 'hpi' named entity 'HeLa' named entity 'microparticle' named entity 'outer membrane' named entity 'actin polymerization' named entity 'isoform' named entity 'actin' named entity 'cell motility' named entity 'isoforms' named entity 'siRNA' named entity 'A549' named entity 'rat' named entity 'Kotwal' named entity 'actin' named entity 'actin' named entity 'virus' named entity 'isoforms' named entity 'actin polymerization' named entity 'virions' named entity 'actin' named entity 'isoforms' named entity 'actin-based motility' named entity 'plaque size' named entity 'virus' named entity 'actin' named entity 'actin-based motility' named entity 'isoform' named entity 'cell lines' named entity 'phenotypes' named entity 'hpi' named entity 'statistical analysis' named entity 'cell motility' named entity 'SDS-PAGE' named entity 'cell types' named entity 'immunofluorescence' named entity 'virus' named entity 'densitometer' named entity 'envelope protein' named entity 'anaphase' named entity 'rat' named entity 'Actin' named entity 'FIJI' named entity 'N-WASP' named entity 'immunoblot' named entity 'Immunoreactive' named entity 'lysis buffer' named entity 'EEV'

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